Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodies
Farmlands fertilized with livestock manure-derived amendments have become a hot topic in the dissemination of antibiotic resistance genes (ARGs). Field ponding water connects rice paddies with surrounding water bodies, such as reservoirs, rivers, and lakes. However, there is a knowledge gap in under...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1135278/full |
| _version_ | 1797867995425406976 |
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| author | Ming-Sha Zhang Si-Zhou Liang Si-Zhou Liang Wei-Guo Zhang Wei-Guo Zhang Wei-Guo Zhang Wei-Guo Zhang Ya-Jun Chang Zhongfang Lei Wen Li Guo-Liang Zhang Yan Gao |
| author_facet | Ming-Sha Zhang Si-Zhou Liang Si-Zhou Liang Wei-Guo Zhang Wei-Guo Zhang Wei-Guo Zhang Wei-Guo Zhang Ya-Jun Chang Zhongfang Lei Wen Li Guo-Liang Zhang Yan Gao |
| author_sort | Ming-Sha Zhang |
| collection | DOAJ |
| description | Farmlands fertilized with livestock manure-derived amendments have become a hot topic in the dissemination of antibiotic resistance genes (ARGs). Field ponding water connects rice paddies with surrounding water bodies, such as reservoirs, rivers, and lakes. However, there is a knowledge gap in understanding whether and how manure-borne ARGs can be transferred from paddy soil into field ponding water. Our studies suggest that the manure-derived ARGs aadA1, bla1, catA1, cmlA1-01, cmx(A), ermB, mepA and tetPB-01 can easily be transferred into field ponding water from paddy soil. The bacterial phyla Crenarchaeota, Verrucomicrobia, Cyanobacteria, Choloroflexi, Acidobacteria, Firmicutes, Bacteroidetes, and Actinobacteria are potential hosts of ARGs. Opportunistic pathogens detected in both paddy soil and field ponding water showed robust correlations with ARGs. Network co-occurrence analysis showed that mobile genetic elements (MGEs) were strongly correlated with ARGs. Our findings highlight that manure-borne ARGs and antibiotic-resistant bacteria in paddy fields can conveniently disseminate to the surrounding waterbodies through field ponding water, posing a threat to public health. This study provides a new perspective for comprehensively assessing the risk posed by ARGs in paddy ecosystems. |
| first_indexed | 2024-04-09T23:49:04Z |
| format | Article |
| id | doaj.art-4b356e9ae4f4487680246d5e43809fbd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T23:49:04Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4b356e9ae4f4487680246d5e43809fbd2023-03-17T15:21:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11352781135278Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodiesMing-Sha Zhang0Si-Zhou Liang1Si-Zhou Liang2Wei-Guo Zhang3Wei-Guo Zhang4Wei-Guo Zhang5Wei-Guo Zhang6Ya-Jun Chang7Zhongfang Lei8Wen Li9Guo-Liang Zhang10Yan Gao11School of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing, ChinaSchool of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, ChinaSchool of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, ChinaSchool of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, ChinaSchool of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing, ChinaSchool of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanSchool of Life Sciences, Nanjing University, Nanjing, ChinaHuaiyin Institute of Technology, Huaian, ChinaInstitute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaFarmlands fertilized with livestock manure-derived amendments have become a hot topic in the dissemination of antibiotic resistance genes (ARGs). Field ponding water connects rice paddies with surrounding water bodies, such as reservoirs, rivers, and lakes. However, there is a knowledge gap in understanding whether and how manure-borne ARGs can be transferred from paddy soil into field ponding water. Our studies suggest that the manure-derived ARGs aadA1, bla1, catA1, cmlA1-01, cmx(A), ermB, mepA and tetPB-01 can easily be transferred into field ponding water from paddy soil. The bacterial phyla Crenarchaeota, Verrucomicrobia, Cyanobacteria, Choloroflexi, Acidobacteria, Firmicutes, Bacteroidetes, and Actinobacteria are potential hosts of ARGs. Opportunistic pathogens detected in both paddy soil and field ponding water showed robust correlations with ARGs. Network co-occurrence analysis showed that mobile genetic elements (MGEs) were strongly correlated with ARGs. Our findings highlight that manure-borne ARGs and antibiotic-resistant bacteria in paddy fields can conveniently disseminate to the surrounding waterbodies through field ponding water, posing a threat to public health. This study provides a new perspective for comprehensively assessing the risk posed by ARGs in paddy ecosystems.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1135278/fullantibiotic resistance genesfield ponding waterrice paddymanure fertilized soilpublic health |
| spellingShingle | Ming-Sha Zhang Si-Zhou Liang Si-Zhou Liang Wei-Guo Zhang Wei-Guo Zhang Wei-Guo Zhang Wei-Guo Zhang Ya-Jun Chang Zhongfang Lei Wen Li Guo-Liang Zhang Yan Gao Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodies Frontiers in Microbiology antibiotic resistance genes field ponding water rice paddy manure fertilized soil public health |
| title | Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodies |
| title_full | Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodies |
| title_fullStr | Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodies |
| title_full_unstemmed | Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodies |
| title_short | Field ponding water exacerbates the dissemination of manure-derived antibiotic resistance genes from paddy soil to surrounding waterbodies |
| title_sort | field ponding water exacerbates the dissemination of manure derived antibiotic resistance genes from paddy soil to surrounding waterbodies |
| topic | antibiotic resistance genes field ponding water rice paddy manure fertilized soil public health |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1135278/full |
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