<italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study
ABSTRACT Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2020-02-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00735-19 |
| _version_ | 1818651953765285888 |
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| author | Xiaoming Bian Jolene M. Garber Kerry K. Cooper Steven Huynh Jennifer Jones Michael K. Mills Daniel Rafala Dilruba Nasrin Karen L. Kotloff Craig T. Parker Sharon M. Tennant William G. Miller Christine M. Szymanski |
| author_facet | Xiaoming Bian Jolene M. Garber Kerry K. Cooper Steven Huynh Jennifer Jones Michael K. Mills Daniel Rafala Dilruba Nasrin Karen L. Kotloff Craig T. Parker Sharon M. Tennant William G. Miller Christine M. Szymanski |
| author_sort | Xiaoming Bian |
| collection | DOAJ |
| description | ABSTRACT Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-Saharan Africa and South Asia, we examined fecal microbial compositions, bacterial isolates, and their carbohydrate metabolic pathways in Campylobacter-positive infants <1 year of age from the Global Enterics Multicenter Study. Exclusively breastfed infants with diarrhea exhibited high Campylobacter abundances, and this negatively correlated with bacterial carbohydrate metabolism. Although C. jejuni and Campylobacter coli are prevalent among these infants, the second most abundant Campylobacter species was a new species, which we named “Candidatus Campylobacter infans.” Asymptomatic Campylobacter carriers also possess significantly different proportions of specific gut microbes compared to diarrheal cases. These findings provide insight into Campylobacter infections in infants in sub-Saharan Africa and South Asia and help inform strategies aimed at eliminating campylobacteriosis in these areas. IMPORTANCE Campylobacter is the primary cause of bacterial diarrhea in the United States and can lead to the development of the postinfectious autoimmune neuropathy known as Guillain-Barré syndrome. Also, drug-resistant campylobacters are becoming a serious concern both locally and abroad. In low- and middle-income countries (LMICs), infection with Campylobacter is linked to high rates of morbidity, growth stunting, and mortality in children, and breastfeeding is important for infant nutrition, development, and protection against infectious diseases. In this study, we examined the relationship between breastfeeding and Campylobacter infection and demonstrate the increased selection for C. jejuni and C. coli strains unable to metabolize fucose. We also identify a new Campylobacter species coinfecting these infants with a high prevalence in five of the seven countries in sub-Saharan Africa and South Asia examined. These findings indicate that more detailed studies are needed in LMICs to understand the Campylobacter infection process in order to devise a strategy for eliminating this pathogenic microbe. |
| first_indexed | 2024-12-17T02:14:18Z |
| format | Article |
| id | doaj.art-4b36dc2e6c2e41a29f5aff605ab1c0f4 |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-17T02:14:18Z |
| publishDate | 2020-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-4b36dc2e6c2e41a29f5aff605ab1c0f42022-12-21T22:07:27ZengAmerican Society for MicrobiologymSphere2379-50422020-02-015110.1128/mSphere.00735-19<italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter StudyXiaoming Bian0Jolene M. Garber1Kerry K. Cooper2Steven Huynh3Jennifer Jones4Michael K. Mills5Daniel Rafala6Dilruba Nasrin7Karen L. Kotloff8Craig T. Parker9Sharon M. Tennant10William G. Miller11Christine M. Szymanski12Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USAComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USASchool of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, Arizona, USAProduce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, USACenter for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USADepartment of Microbiology, University of Georgia, Athens, Georgia, USADepartment of Microbiology, University of Georgia, Athens, Georgia, USACenter for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USACenter for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USAProduce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, USACenter for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USAProduce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, USAComplex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USAABSTRACT Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-Saharan Africa and South Asia, we examined fecal microbial compositions, bacterial isolates, and their carbohydrate metabolic pathways in Campylobacter-positive infants <1 year of age from the Global Enterics Multicenter Study. Exclusively breastfed infants with diarrhea exhibited high Campylobacter abundances, and this negatively correlated with bacterial carbohydrate metabolism. Although C. jejuni and Campylobacter coli are prevalent among these infants, the second most abundant Campylobacter species was a new species, which we named “Candidatus Campylobacter infans.” Asymptomatic Campylobacter carriers also possess significantly different proportions of specific gut microbes compared to diarrheal cases. These findings provide insight into Campylobacter infections in infants in sub-Saharan Africa and South Asia and help inform strategies aimed at eliminating campylobacteriosis in these areas. IMPORTANCE Campylobacter is the primary cause of bacterial diarrhea in the United States and can lead to the development of the postinfectious autoimmune neuropathy known as Guillain-Barré syndrome. Also, drug-resistant campylobacters are becoming a serious concern both locally and abroad. In low- and middle-income countries (LMICs), infection with Campylobacter is linked to high rates of morbidity, growth stunting, and mortality in children, and breastfeeding is important for infant nutrition, development, and protection against infectious diseases. In this study, we examined the relationship between breastfeeding and Campylobacter infection and demonstrate the increased selection for C. jejuni and C. coli strains unable to metabolize fucose. We also identify a new Campylobacter species coinfecting these infants with a high prevalence in five of the seven countries in sub-Saharan Africa and South Asia examined. These findings indicate that more detailed studies are needed in LMICs to understand the Campylobacter infection process in order to devise a strategy for eliminating this pathogenic microbe.https://journals.asm.org/doi/10.1128/mSphere.00735-19CampylobacterbreastfeedingGEMSl-fucose metabolism“Candidatus Campylobacter infans,” gut microbiome |
| spellingShingle | Xiaoming Bian Jolene M. Garber Kerry K. Cooper Steven Huynh Jennifer Jones Michael K. Mills Daniel Rafala Dilruba Nasrin Karen L. Kotloff Craig T. Parker Sharon M. Tennant William G. Miller Christine M. Szymanski <italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study mSphere Campylobacter breastfeeding GEMS l-fucose metabolism “Candidatus Campylobacter infans,” gut microbiome |
| title | <italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study |
| title_full | <italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study |
| title_fullStr | <italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study |
| title_full_unstemmed | <italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study |
| title_short | <italic toggle="yes">Campylobacter</italic> Abundance in Breastfed Infants and Identification of a New Species in the Global Enterics Multicenter Study |
| title_sort | italic toggle yes campylobacter italic abundance in breastfed infants and identification of a new species in the global enterics multicenter study |
| topic | Campylobacter breastfeeding GEMS l-fucose metabolism “Candidatus Campylobacter infans,” gut microbiome |
| url | https://journals.asm.org/doi/10.1128/mSphere.00735-19 |
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