Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens
This study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, digesta pH, ileal populations of Clostridium perfringens and lactic acid bacteria, ileal nutrient digestibility, digestive enzyme activity, and mRNA expression of intestinal nutrient transporters in starter...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2017-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/54/2/54_0160099/_pdf/-char/en |
| _version_ | 1827939002966081536 |
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| author | Seyed Mohammad Hosseini Milad Manafi Hasan Nazarizadeh |
| author_facet | Seyed Mohammad Hosseini Milad Manafi Hasan Nazarizadeh |
| author_sort | Seyed Mohammad Hosseini |
| collection | DOAJ |
| description | This study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, digesta pH, ileal populations of Clostridium perfringens and lactic acid bacteria, ileal nutrient digestibility, digestive enzyme activity, and mRNA expression of intestinal nutrient transporters in starter broilers challenged with C. perfringens. The experiment was conducted in a 2×2 factorial arrangement with two levels of CA (0 and 30 g/kg) and 2 levels of xylanase (0 and 200 mg/kg). Each of the four dietary treatments was fed to six replicate pens (15 birds/pen) between 0 and 21 d of age. Dietary CA significantly increased ADFI and ADG; meanwhile, xylanase addition led to a substantial reduction in FCR (P<0.05). No differences in digesta pH, C. perfringens counts, or quantity of lactic acid bacteria were found between the treatments. Xylanase supplementation increased AME values (P<0.01) and ileal digestibility of CP (P<0.05) in challenged birds. The inclusion of CA also increased the AME (P<0.01), and tended to increased ileal CP digestibility (P＝0.085). Xylanase supplementation increased α-amylase, trypsin, and sucrose activity in the jejunum (P<0.01). Dietary CA significantly increased (P<0.01) villi length as well as the villus length to crypt depth ratio in jejunum segments. The jejunal mRNA expression of sodium glucose co-transporter 1 (SGLT1) and H+-dependent peptide transporter 1 (PepT1) were upregulated by xylanase supplementation (P<0.01). The results suggest that dietary CA can promote growth as well as improve intestinal morphology and AME in birds challenged with necrotic enteritis. This study shows that xylanase supplementation improved FCR and AME in birds independent of C. perfringens infection; it also elevated the apparent ileal digestibility of CP, digestive enzyme activities, and mRNA expression of nutrient transporters in challenged birds. |
| first_indexed | 2024-03-13T08:46:00Z |
| format | Article |
| id | doaj.art-4b3714e94a434c6c996bab7bd5e619b1 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:00Z |
| publishDate | 2017-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-4b3714e94a434c6c996bab7bd5e619b12023-05-30T02:23:08ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862017-04-0154214915610.2141/jpsa.0160099jpsaEffects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringensSeyed Mohammad Hosseini0Milad Manafi1Hasan Nazarizadeh2Department of Animal Science, Faculty of Agriculture, University of Birjand, IranDepartment of Animal Science, Faculty of Agriculture, Malayer University, IranDepartment of Animal Science, Faculty of Agriculture, University of Birjand, IranThis study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, digesta pH, ileal populations of Clostridium perfringens and lactic acid bacteria, ileal nutrient digestibility, digestive enzyme activity, and mRNA expression of intestinal nutrient transporters in starter broilers challenged with C. perfringens. The experiment was conducted in a 2×2 factorial arrangement with two levels of CA (0 and 30 g/kg) and 2 levels of xylanase (0 and 200 mg/kg). Each of the four dietary treatments was fed to six replicate pens (15 birds/pen) between 0 and 21 d of age. Dietary CA significantly increased ADFI and ADG; meanwhile, xylanase addition led to a substantial reduction in FCR (P<0.05). No differences in digesta pH, C. perfringens counts, or quantity of lactic acid bacteria were found between the treatments. Xylanase supplementation increased AME values (P<0.01) and ileal digestibility of CP (P<0.05) in challenged birds. The inclusion of CA also increased the AME (P<0.01), and tended to increased ileal CP digestibility (P＝0.085). Xylanase supplementation increased α-amylase, trypsin, and sucrose activity in the jejunum (P<0.01). Dietary CA significantly increased (P<0.01) villi length as well as the villus length to crypt depth ratio in jejunum segments. The jejunal mRNA expression of sodium glucose co-transporter 1 (SGLT1) and H+-dependent peptide transporter 1 (PepT1) were upregulated by xylanase supplementation (P<0.01). The results suggest that dietary CA can promote growth as well as improve intestinal morphology and AME in birds challenged with necrotic enteritis. This study shows that xylanase supplementation improved FCR and AME in birds independent of C. perfringens infection; it also elevated the apparent ileal digestibility of CP, digestive enzyme activities, and mRNA expression of nutrient transporters in challenged birds.https://www.jstage.jst.go.jp/article/jpsa/54/2/54_0160099/_pdf/-char/encitric acidnecrotic enteritisnutrient digestibilitynutrient transporterxylanase |
| spellingShingle | Seyed Mohammad Hosseini Milad Manafi Hasan Nazarizadeh Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens The Journal of Poultry Science citric acid necrotic enteritis nutrient digestibility nutrient transporter xylanase |
| title | Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens |
| title_full | Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens |
| title_fullStr | Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens |
| title_full_unstemmed | Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens |
| title_short | Effects of Xylanase Supplementation and Citric Acid on Performance, Ileal Nutrients Digestibility, and Gene Expression of Intestinal Nutrient Transporters in Broilers Challenged with Clostridium perfringens |
| title_sort | effects of xylanase supplementation and citric acid on performance ileal nutrients digestibility and gene expression of intestinal nutrient transporters in broilers challenged with clostridium perfringens |
| topic | citric acid necrotic enteritis nutrient digestibility nutrient transporter xylanase |
| url | https://www.jstage.jst.go.jp/article/jpsa/54/2/54_0160099/_pdf/-char/en |
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