Surface modifications of a silicalite film designed for coating orthopaedic implants
The effect of various surface modifications of silicalite-1 film grown on a Ti6Al4V alloy on biocompatibility and cell differentiation has been addressed. The as-synthesized silicalite film was first modified by alkaline etching and by subsequent treatments in oxygen radiofrequency (O2 RF) plasma, b...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522009959 |
| _version_ | 1811292658204672000 |
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| author | Antonín Brož Ivan Jirka Roman Matějka Jana Štěpanovská Martina Doubková Petr Sajdl Jan Drahokoupil Oleksandr Volochanskyi Terézia Futóová Lucie Bačáková |
| author_facet | Antonín Brož Ivan Jirka Roman Matějka Jana Štěpanovská Martina Doubková Petr Sajdl Jan Drahokoupil Oleksandr Volochanskyi Terézia Futóová Lucie Bačáková |
| author_sort | Antonín Brož |
| collection | DOAJ |
| description | The effect of various surface modifications of silicalite-1 film grown on a Ti6Al4V alloy on biocompatibility and cell differentiation has been addressed. The as-synthesized silicalite film was first modified by alkaline etching and by subsequent treatments in oxygen radiofrequency (O2 RF) plasma, by calcination at 500 °C, and by processing the calcinated samples in the O2 RF plasma. The chemical composition, crystallinity, topography and wettability of samples were characterized using angle-resolved X-ray photoelectron spectroscopy, X-ray diffraction, scanning electron microscopy, and sessile water drop contact angle measurements. We have discovered the presence of a non-zeolitic defective SiOx phase (NZP) with coordinated hydrocarbon chains. The NZP can be removed by the exposition to the O2 RF plasma. The metabolic activity tests done on human osteoblast line Saos-2 revealed that the NZP can influence cell proliferation; however, all studied modifications of silicalite film can be considered biocompatible. The osteogenic differentiation was studied on human mesenchymal stem cells under static conditions and under dynamic (pulsatile and shear) stress. The results obtained by the real-time qPCR and immunofluorescence microscopy suggest that etched, plasma-treated and calcinated + plasma-treated samples are the most promising for osteogenic cell differentiation. |
| first_indexed | 2024-04-13T04:49:37Z |
| format | Article |
| id | doaj.art-4b3cd51ab804416cbcd48c63d6dd0907 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-13T04:49:37Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-4b3cd51ab804416cbcd48c63d6dd09072022-12-22T03:01:44ZengElsevierMaterials & Design0264-12752022-12-01224111373Surface modifications of a silicalite film designed for coating orthopaedic implantsAntonín Brož0Ivan Jirka1Roman Matějka2Jana Štěpanovská3Martina Doubková4Petr Sajdl5Jan Drahokoupil6Oleksandr Volochanskyi7Terézia Futóová8Lucie Bačáková9Institute of Physiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic; Corresponding authors.J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, v.v.i., Dolejškova 3, 182 23 Prague 8, Czech Republic; Corresponding authors.Institute of Physiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic; Dept. of Biomedical Technology, Faculty of Biomedical Engineering, Czech Technical University in Prague, nám. Sítná 3105, 272 01 Kladno, Czech RepublicInstitute of Physiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech Republic; Dept. of Biomedical Technology, Faculty of Biomedical Engineering, Czech Technical University in Prague, nám. Sítná 3105, 272 01 Kladno, Czech RepublicInstitute of Physiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech RepublicUniversity of Chemistry and Technology, Department of Power Engineering, Technická 3, 166 28 Prague 6, Czech RepublicInstitute of Physics of the Czech Academy of Sciences, v.v.i., Na Slovance 1999-2, 182 21 Prague 8, Czech RepublicJ. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, v.v.i., Dolejškova 3, 182 23 Prague 8, Czech RepublicInstitute of Physiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech RepublicInstitute of Physiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20 Prague 4, Czech RepublicThe effect of various surface modifications of silicalite-1 film grown on a Ti6Al4V alloy on biocompatibility and cell differentiation has been addressed. The as-synthesized silicalite film was first modified by alkaline etching and by subsequent treatments in oxygen radiofrequency (O2 RF) plasma, by calcination at 500 °C, and by processing the calcinated samples in the O2 RF plasma. The chemical composition, crystallinity, topography and wettability of samples were characterized using angle-resolved X-ray photoelectron spectroscopy, X-ray diffraction, scanning electron microscopy, and sessile water drop contact angle measurements. We have discovered the presence of a non-zeolitic defective SiOx phase (NZP) with coordinated hydrocarbon chains. The NZP can be removed by the exposition to the O2 RF plasma. The metabolic activity tests done on human osteoblast line Saos-2 revealed that the NZP can influence cell proliferation; however, all studied modifications of silicalite film can be considered biocompatible. The osteogenic differentiation was studied on human mesenchymal stem cells under static conditions and under dynamic (pulsatile and shear) stress. The results obtained by the real-time qPCR and immunofluorescence microscopy suggest that etched, plasma-treated and calcinated + plasma-treated samples are the most promising for osteogenic cell differentiation.http://www.sciencedirect.com/science/article/pii/S0264127522009959SilicaliteSynthesisBiocompatibilitySurface modificationCell differentiationImplants |
| spellingShingle | Antonín Brož Ivan Jirka Roman Matějka Jana Štěpanovská Martina Doubková Petr Sajdl Jan Drahokoupil Oleksandr Volochanskyi Terézia Futóová Lucie Bačáková Surface modifications of a silicalite film designed for coating orthopaedic implants Materials & Design Silicalite Synthesis Biocompatibility Surface modification Cell differentiation Implants |
| title | Surface modifications of a silicalite film designed for coating orthopaedic implants |
| title_full | Surface modifications of a silicalite film designed for coating orthopaedic implants |
| title_fullStr | Surface modifications of a silicalite film designed for coating orthopaedic implants |
| title_full_unstemmed | Surface modifications of a silicalite film designed for coating orthopaedic implants |
| title_short | Surface modifications of a silicalite film designed for coating orthopaedic implants |
| title_sort | surface modifications of a silicalite film designed for coating orthopaedic implants |
| topic | Silicalite Synthesis Biocompatibility Surface modification Cell differentiation Implants |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522009959 |
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