Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional Plant
The volatile oils hydrodistilled from the aerial parts and roots of <i>O. carduchorum</i> C.C Towns. (Fabaceae) have been chemically characterized for the first time. A total of 43 constituents with an abundance >0.03% were identified and quantified in the two oils by GC/MS and GC/FID...
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MDPI AG
2023-08-01
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| author | Hawraz Ibrahim M. Amin Kamaran Abdoulrahman Azad S. Sadraddin Heman A. Smail Zanko Hassan Jawhar Kovan Dilawer Issa Chabaco Armijos Giovanni Vidari |
| author_facet | Hawraz Ibrahim M. Amin Kamaran Abdoulrahman Azad S. Sadraddin Heman A. Smail Zanko Hassan Jawhar Kovan Dilawer Issa Chabaco Armijos Giovanni Vidari |
| author_sort | Hawraz Ibrahim M. Amin |
| collection | DOAJ |
| description | The volatile oils hydrodistilled from the aerial parts and roots of <i>O. carduchorum</i> C.C Towns. (Fabaceae) have been chemically characterized for the first time. A total of 43 constituents with an abundance >0.03% were identified and quantified in the two oils by GC/MS and GC/FID analyses. They comprise 38 components (98.58%) of the oil isolated from the aerial parts (OCA) and 34 components (93.33%) of the oil from the roots (OCR). Six constituents, α-pinene (23.11 ± 0.1%), β-elemene (17.33 ± 0.1%), 1,8-cineole (12.15 ± 0.2%), furfural (7.91 ± 0.1%), terpineol-4-ol (6.32 ± 0.2%), and limonene (4.13 ± 0.1%), accounted for about 75% of the total OCA oil. On the other hand, 1,8-cineole (15.79 ± 0.1%), furfural (10.44 ± 0.1%), β-elemene (10.14 ± 0.2%), α-terpineol (7.74 ± 0.1%), linalool (7.45 ± 0.1%), and α-pinene (4.76 ± 0.1%) made up about 60% of the OCR oil. The IC<sub>50</sub> values of the scavenging activities of the OCA and OCR oils towards the DPPH radical and H<sub>2</sub>O<sub>2</sub> were 79.8 ± 0.5 and 153.3 ± 0.6 μg/mL and 394.09 ± 0.2 and 311.67 ± 0.4 μg/mL, respectively. In addition, in the MTS assay, the OCA and OCR oils showed significant antiproliferative effects against T47D, MDA-MB-453, BG-1, and A549 human cancer cells that were more powerful than those against two normal human cell lines, HEK-293 and HFF-1. The abundant presence of β-elemene as an antiproliferative component of the two oils suggested the existence of a new chemotype of <i>O. carduchorum</i>. |
| first_indexed | 2024-03-10T23:38:29Z |
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| spelling | doaj.art-4b49487259334a66b767954a1a17e13e2023-11-19T02:42:27ZengMDPI AGPlants2223-77472023-08-011216301310.3390/plants12163013Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional PlantHawraz Ibrahim M. Amin0Kamaran Abdoulrahman1Azad S. Sadraddin2Heman A. Smail3Zanko Hassan Jawhar4Kovan Dilawer Issa5Chabaco Armijos6Giovanni Vidari7Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil 44001, IraqDepartment of Chemistry, College of Science, Salahaddin University-Erbil, Erbil 44001, IraqDepartment of Chemistry, College of Education, Salahaddin University-Erbil, Erbil 44001, IraqDepartment of Chemistry, College of Science, Salahaddin University-Erbil, Erbil 44001, IraqDepartment of Science, College of Health Science, Lebanese French University, Erbil 44001, IraqDepartment of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil 44001, IraqDepartamento de Química, Universidad Técnica Particular de Loja (UTPL), Loja 1101608, EcuadorDepartment of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil 44001, IraqThe volatile oils hydrodistilled from the aerial parts and roots of <i>O. carduchorum</i> C.C Towns. (Fabaceae) have been chemically characterized for the first time. A total of 43 constituents with an abundance >0.03% were identified and quantified in the two oils by GC/MS and GC/FID analyses. They comprise 38 components (98.58%) of the oil isolated from the aerial parts (OCA) and 34 components (93.33%) of the oil from the roots (OCR). Six constituents, α-pinene (23.11 ± 0.1%), β-elemene (17.33 ± 0.1%), 1,8-cineole (12.15 ± 0.2%), furfural (7.91 ± 0.1%), terpineol-4-ol (6.32 ± 0.2%), and limonene (4.13 ± 0.1%), accounted for about 75% of the total OCA oil. On the other hand, 1,8-cineole (15.79 ± 0.1%), furfural (10.44 ± 0.1%), β-elemene (10.14 ± 0.2%), α-terpineol (7.74 ± 0.1%), linalool (7.45 ± 0.1%), and α-pinene (4.76 ± 0.1%) made up about 60% of the OCR oil. The IC<sub>50</sub> values of the scavenging activities of the OCA and OCR oils towards the DPPH radical and H<sub>2</sub>O<sub>2</sub> were 79.8 ± 0.5 and 153.3 ± 0.6 μg/mL and 394.09 ± 0.2 and 311.67 ± 0.4 μg/mL, respectively. In addition, in the MTS assay, the OCA and OCR oils showed significant antiproliferative effects against T47D, MDA-MB-453, BG-1, and A549 human cancer cells that were more powerful than those against two normal human cell lines, HEK-293 and HFF-1. The abundant presence of β-elemene as an antiproliferative component of the two oils suggested the existence of a new chemotype of <i>O. carduchorum</i>.https://www.mdpi.com/2223-7747/12/16/3013<i>Onobrychis carduchorum</i>FabaceaeKurdish traditional medicineessential oilsantiradicalantioxidant |
| spellingShingle | Hawraz Ibrahim M. Amin Kamaran Abdoulrahman Azad S. Sadraddin Heman A. Smail Zanko Hassan Jawhar Kovan Dilawer Issa Chabaco Armijos Giovanni Vidari Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional Plant Plants <i>Onobrychis carduchorum</i> Fabaceae Kurdish traditional medicine essential oils antiradical antioxidant |
| title | Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional Plant |
| title_full | Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional Plant |
| title_fullStr | Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional Plant |
| title_full_unstemmed | Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional Plant |
| title_short | Chemical Composition and In Vitro Evaluation of Antioxidant and Antiproliferative Effects of Volatile Oils Hydrodistilled from <i>Onobrychis carduchorum</i> C.C. Towns., a Kurdish Traditional Plant |
| title_sort | chemical composition and in vitro evaluation of antioxidant and antiproliferative effects of volatile oils hydrodistilled from i onobrychis carduchorum i c c towns a kurdish traditional plant |
| topic | <i>Onobrychis carduchorum</i> Fabaceae Kurdish traditional medicine essential oils antiradical antioxidant |
| url | https://www.mdpi.com/2223-7747/12/16/3013 |
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