Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria
This study was conducted to isolate and detect methicillin-resistant staphylococci (MRS) in healthy broilers in Nsukka Southeast, Nigeria and determine the antibiogram of the isolates. Cloacal and skin swabs were collected from each of 101 randomly sampled broilers meant for slaughter. The samples w...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2015-03-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9479 |
| _version_ | 1818000794292584448 |
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| author | Ifeoma Chinyere UGWU Madubuike Umunna ANYANWU Chidozie Clifford UGWU Jude Chukwuemeka OKORO |
| author_facet | Ifeoma Chinyere UGWU Madubuike Umunna ANYANWU Chidozie Clifford UGWU Jude Chukwuemeka OKORO |
| author_sort | Ifeoma Chinyere UGWU |
| collection | DOAJ |
| description | This study was conducted to isolate and detect methicillin-resistant staphylococci (MRS) in healthy broilers in Nsukka Southeast, Nigeria and determine the antibiogram of the isolates. Cloacal and skin swabs were collected from each of 101 randomly sampled broilers meant for slaughter. The samples were processed for isolation and identification of methicillin-resistant Staphylococcus species, following standard methods. Confirmation of methicillin-resistance by the isolates was done using penicillin binding protein 2a (PBP2a) kit. Phenotypic resistance of the isolates to antimicrobial agents was determined using disc diffusion method. Out of 202 samples processed, 200 (99.01%) yielded positive growth of staphylococci on oxacillin-supplemented oxacillin-resistance staphylococcal agar base (ORSAB). A total of 200 methicillin-resistant staphylococcal isolates were obtained. Of these, 91 (45.5%) were identified as methicillin-resistant coagulase-positive Staphylococcus (MRCoPS), while 109 (54.5%) were identified as methicillin-resistant coagulase-negative Staphylococcus species (MRCoNS). Out of the 91 MRCoPS, 53 (58.2%) were identified as methicillin-resistant Staphylococcus aureus (MRSA). Resistance of the isolates was 99.5% to erythromycin and chloramphenicol, 100% to oxacillin, 76.5% to gentamicin, 96.5% to clindamycin, 92.5% to ciprofloxacin, 99% to sulphamethoxazole/trimethoprim and tetracycline, and 98.5% to streptomycin and cefoxitin. All the isolates were multidrug resistant. This study has shown that healthy broilers reared and slaughtered in Nsukka Southeast, Nigeria harbour multidrug-resistant MRS and thus serve as their reservoirs. |
| first_indexed | 2024-04-14T03:26:12Z |
| format | Article |
| id | doaj.art-4b4f92d9149244c38cacba9bb3301cdd |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T03:26:12Z |
| publishDate | 2015-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-4b4f92d9149244c38cacba9bb3301cdd2022-12-22T02:15:10ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642015-03-0171202510.15835/nsb7194798112Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, NigeriaIfeoma Chinyere UGWU0Madubuike Umunna ANYANWU1Chidozie Clifford UGWU2Jude Chukwuemeka OKORO3University of Nigeria, Nsukka, Faculty of Veterinary Medicine, Department of Veterinary Pathology and Microbiology, Enugu StateUniversity of Nigeria, Nsukka, Faculty of Veterinary Medicine, Department of Veterinary Pathology and Microbiology, Enugu StateFederal University of Technology, Department of Animal Science and Technology, OwerriUniversity of Nigeria, Nsukka, Faculty of Veterinary Medicine, Department of Veterinary Pathology and Microbiology, Enugu StateThis study was conducted to isolate and detect methicillin-resistant staphylococci (MRS) in healthy broilers in Nsukka Southeast, Nigeria and determine the antibiogram of the isolates. Cloacal and skin swabs were collected from each of 101 randomly sampled broilers meant for slaughter. The samples were processed for isolation and identification of methicillin-resistant Staphylococcus species, following standard methods. Confirmation of methicillin-resistance by the isolates was done using penicillin binding protein 2a (PBP2a) kit. Phenotypic resistance of the isolates to antimicrobial agents was determined using disc diffusion method. Out of 202 samples processed, 200 (99.01%) yielded positive growth of staphylococci on oxacillin-supplemented oxacillin-resistance staphylococcal agar base (ORSAB). A total of 200 methicillin-resistant staphylococcal isolates were obtained. Of these, 91 (45.5%) were identified as methicillin-resistant coagulase-positive Staphylococcus (MRCoPS), while 109 (54.5%) were identified as methicillin-resistant coagulase-negative Staphylococcus species (MRCoNS). Out of the 91 MRCoPS, 53 (58.2%) were identified as methicillin-resistant Staphylococcus aureus (MRSA). Resistance of the isolates was 99.5% to erythromycin and chloramphenicol, 100% to oxacillin, 76.5% to gentamicin, 96.5% to clindamycin, 92.5% to ciprofloxacin, 99% to sulphamethoxazole/trimethoprim and tetracycline, and 98.5% to streptomycin and cefoxitin. All the isolates were multidrug resistant. This study has shown that healthy broilers reared and slaughtered in Nsukka Southeast, Nigeria harbour multidrug-resistant MRS and thus serve as their reservoirs.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9479 |
| spellingShingle | Ifeoma Chinyere UGWU Madubuike Umunna ANYANWU Chidozie Clifford UGWU Jude Chukwuemeka OKORO Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria Notulae Scientia Biologicae |
| title | Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria |
| title_full | Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria |
| title_fullStr | Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria |
| title_full_unstemmed | Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria |
| title_short | Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria |
| title_sort | isolation and detection of methicillin resistant staphylococci in healthy broilers in nsukka southeast nigeria |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9479 |
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