In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging
IntroductionTransplantation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is a promising treatment for heart failure. Information on long-term cell engraftment after transplantation is clinically important. However, clinically applicable evaluation methods have not yet bee...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1261330/full |
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| author | Yukihiro Saito Naoko Nose Toshihiro Iida Kaoru Akazawa Takayuki Kanno Yuki Fujimoto Takanori Sasaki Masaru Akehi Takahiro Higuchi Takahiro Higuchi Takahiro Higuchi Satoshi Akagi Masashi Yoshida Toru Miyoshi Hiroshi Ito Kazufumi Nakamura |
| author_facet | Yukihiro Saito Naoko Nose Toshihiro Iida Kaoru Akazawa Takayuki Kanno Yuki Fujimoto Takanori Sasaki Masaru Akehi Takahiro Higuchi Takahiro Higuchi Takahiro Higuchi Satoshi Akagi Masashi Yoshida Toru Miyoshi Hiroshi Ito Kazufumi Nakamura |
| author_sort | Yukihiro Saito |
| collection | DOAJ |
| description | IntroductionTransplantation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is a promising treatment for heart failure. Information on long-term cell engraftment after transplantation is clinically important. However, clinically applicable evaluation methods have not yet been established.MethodsIn this study, to noninvasively assess transplanted cell engraftment, human SLC5A5, which encodes a sodium/iodide symporter (NIS) that transports radioactive tracers such as 125I, 18F-tetrafluoroborate (TFB), and 99mTc-pertechnetate (99mTcO4−), was transduced into human induced pluripotent stem cells (iPSCs), and nuclear medicine imaging was used to track engrafted human iPSC-CMs.ResultsTo evaluate the pluripotency of NIS-expressing human iPSCs, they were subcutaneously transplanted into immunodeficient rats. Teratomas were detected by 99mTcO4− single photon emission computed tomography (SPECT/CT) imaging. NIS expression and the uptake ability of 125I were maintained in purified human iPSC-CMs. NIS-expressing human iPSC-CMs transplanted into immunodeficient rats could be detected over time using 99mTcO4− SPECT/CT imaging. Unexpectedly, NIS expression affected cell proliferation of human iPSCs and iPSC-derived cells.DiscussionSuch functionally designed iPSC-CMs have potential clinical applications as a noninvasive method of grafted cell evaluation, but further studies are needed to determine the effects of NIS transduction on cellular characteristics and functions. |
| first_indexed | 2024-03-12T02:02:06Z |
| format | Article |
| id | doaj.art-4b50470f16fc41e0995e29db4a77eded |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-03-12T02:02:06Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-4b50470f16fc41e0995e29db4a77eded2023-09-07T11:11:56ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2023-09-011010.3389/fcvm.2023.12613301261330In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imagingYukihiro Saito0Naoko Nose1Toshihiro Iida2Kaoru Akazawa3Takayuki Kanno4Yuki Fujimoto5Takanori Sasaki6Masaru Akehi7Takahiro Higuchi8Takahiro Higuchi9Takahiro Higuchi10Satoshi Akagi11Masashi Yoshida12Toru Miyoshi13Hiroshi Ito14Kazufumi Nakamura15Department of Cardiovascular Medicine, Okayama University Hospital, Okayama, JapanMolecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanDepartment of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanDepartment of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanMolecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanMolecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanOkayama Medical Innovation Center, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanOkayama Medical Innovation Center, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanMolecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanDepartment of Nuclear Medicine, University Hospital Würzburg, Würzburg, GermanyComprehensive Heart Failure Center, University Hospital Würzburg, Würzburg, GermanyDepartment of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanDepartment of Chronic Kidney Disease and Cardiovascular Disease, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanDepartment of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanDepartment of General Internal Medicine 3, Kawasaki Medical School, Okayama, JapanDepartment of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, JapanIntroductionTransplantation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is a promising treatment for heart failure. Information on long-term cell engraftment after transplantation is clinically important. However, clinically applicable evaluation methods have not yet been established.MethodsIn this study, to noninvasively assess transplanted cell engraftment, human SLC5A5, which encodes a sodium/iodide symporter (NIS) that transports radioactive tracers such as 125I, 18F-tetrafluoroborate (TFB), and 99mTc-pertechnetate (99mTcO4−), was transduced into human induced pluripotent stem cells (iPSCs), and nuclear medicine imaging was used to track engrafted human iPSC-CMs.ResultsTo evaluate the pluripotency of NIS-expressing human iPSCs, they were subcutaneously transplanted into immunodeficient rats. Teratomas were detected by 99mTcO4− single photon emission computed tomography (SPECT/CT) imaging. NIS expression and the uptake ability of 125I were maintained in purified human iPSC-CMs. NIS-expressing human iPSC-CMs transplanted into immunodeficient rats could be detected over time using 99mTcO4− SPECT/CT imaging. Unexpectedly, NIS expression affected cell proliferation of human iPSCs and iPSC-derived cells.DiscussionSuch functionally designed iPSC-CMs have potential clinical applications as a noninvasive method of grafted cell evaluation, but further studies are needed to determine the effects of NIS transduction on cellular characteristics and functions.https://www.frontiersin.org/articles/10.3389/fcvm.2023.1261330/fullsodium/iodide symporterhuman induced pluripotent stem cell-derived cardiomyocytessingle photon emission computed tomographycell-based therapyin vivo imaging |
| spellingShingle | Yukihiro Saito Naoko Nose Toshihiro Iida Kaoru Akazawa Takayuki Kanno Yuki Fujimoto Takanori Sasaki Masaru Akehi Takahiro Higuchi Takahiro Higuchi Takahiro Higuchi Satoshi Akagi Masashi Yoshida Toru Miyoshi Hiroshi Ito Kazufumi Nakamura In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging Frontiers in Cardiovascular Medicine sodium/iodide symporter human induced pluripotent stem cell-derived cardiomyocytes single photon emission computed tomography cell-based therapy in vivo imaging |
| title | In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging |
| title_full | In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging |
| title_fullStr | In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging |
| title_full_unstemmed | In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging |
| title_short | In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging |
| title_sort | in vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging |
| topic | sodium/iodide symporter human induced pluripotent stem cell-derived cardiomyocytes single photon emission computed tomography cell-based therapy in vivo imaging |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1261330/full |
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