Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy
In this study, we investigated the effects of Agardhiella subulata extract (ASE) and its underlying mechanisms in vivo and in vitro. Rats induced hepatic fibrosis with CCl4 and transforming growth factor-β1 (TGF-β1) activated rat hepatic stellate cell line (HSC-T6) was used. The results showed that...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622002961 |
| _version_ | 1818009652655292416 |
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| author | Choirul Anwar Mei-Ling Tsai Bio-Nain Chen Li-Yun Hsu Ching-Shu Lai |
| author_facet | Choirul Anwar Mei-Ling Tsai Bio-Nain Chen Li-Yun Hsu Ching-Shu Lai |
| author_sort | Choirul Anwar |
| collection | DOAJ |
| description | In this study, we investigated the effects of Agardhiella subulata extract (ASE) and its underlying mechanisms in vivo and in vitro. Rats induced hepatic fibrosis with CCl4 and transforming growth factor-β1 (TGF-β1) activated rat hepatic stellate cell line (HSC-T6) was used. The results showed that ASE (oral, 10 or 50 mg/kg) effectively attenuated liver fibrogenesis in the rats. This was evidenced by decreases in serum glutamic oxaloacetic transaminase and glutamic pyruvic transaminase levels, histopathological changes, decreased collagen deposition, and extracellular matrix formation. ASE significantly reduced hepatocyte apoptosis, Kupffer cell activation, and the levels of fibrogenic molecules such as alpha-smooth muscle actin and collagen. Furthermore, ASE downregulated the TGF-β1/Smad signaling pathway and concomitantly inhibited the activation of hepatic stellate cells by reducing autophagy and inducing the regeneration of lipid droplets both in vivo and in vitro. Overall, ASE inhibited various mechanisms that are important targets in the prevention of hepatic fibrosis. |
| first_indexed | 2024-04-14T05:45:36Z |
| format | Article |
| id | doaj.art-4b51a6c3e5db4cdeac29b6af52dee938 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-14T05:45:36Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-4b51a6c3e5db4cdeac29b6af52dee9382022-12-22T02:09:18ZengElsevierJournal of Functional Foods1756-46462022-09-0196105226Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagyChoirul Anwar0Mei-Ling Tsai1Bio-Nain Chen2Li-Yun Hsu3Ching-Shu Lai4Institute of Aquatic Science and Technology, Collage of Hydrosphere Science, National Kaohsiung University of Science and Technology, No.142, Haijhuan Rd., Nanzih Dist., Kaohsiung City 81157, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No.142, Haijhuan Rd., Nanzih Dist., Kaohsiung City 81157, TaiwanDepartment and Graduate Institute of Aquaculture, National Kaohsiung University of Science and Technology, No.142, Haijhuan Rd., Nanzih Dist., Kaohsiung City 81157, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No.142, Haijhuan Rd., Nanzih Dist., Kaohsiung City 81157, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, No.142, Haijhuan Rd., Nanzih Dist., Kaohsiung City 81157, Taiwan; Corresponding author at: Haijhuan Rd., Nanzih District, Kaohsiung, Taiwan.In this study, we investigated the effects of Agardhiella subulata extract (ASE) and its underlying mechanisms in vivo and in vitro. Rats induced hepatic fibrosis with CCl4 and transforming growth factor-β1 (TGF-β1) activated rat hepatic stellate cell line (HSC-T6) was used. The results showed that ASE (oral, 10 or 50 mg/kg) effectively attenuated liver fibrogenesis in the rats. This was evidenced by decreases in serum glutamic oxaloacetic transaminase and glutamic pyruvic transaminase levels, histopathological changes, decreased collagen deposition, and extracellular matrix formation. ASE significantly reduced hepatocyte apoptosis, Kupffer cell activation, and the levels of fibrogenic molecules such as alpha-smooth muscle actin and collagen. Furthermore, ASE downregulated the TGF-β1/Smad signaling pathway and concomitantly inhibited the activation of hepatic stellate cells by reducing autophagy and inducing the regeneration of lipid droplets both in vivo and in vitro. Overall, ASE inhibited various mechanisms that are important targets in the prevention of hepatic fibrosis.http://www.sciencedirect.com/science/article/pii/S1756464622002961Agardhiella subulataAutophagyLipid dropletHepatic stellate cellHepatic fibrosis |
| spellingShingle | Choirul Anwar Mei-Ling Tsai Bio-Nain Chen Li-Yun Hsu Ching-Shu Lai Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy Journal of Functional Foods Agardhiella subulata Autophagy Lipid droplet Hepatic stellate cell Hepatic fibrosis |
| title | Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy |
| title_full | Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy |
| title_fullStr | Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy |
| title_full_unstemmed | Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy |
| title_short | Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy |
| title_sort | molecular mechanisms of agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy |
| topic | Agardhiella subulata Autophagy Lipid droplet Hepatic stellate cell Hepatic fibrosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622002961 |
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