Galactosaminoglycans from normal myometrium and leiomyoma
In many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of c...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2001-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500011 |
| _version_ | 1818409032193409024 |
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| author | A.G.A. Berto S.M. Oba Y.M. Michelacci L.O. Sampaio |
| author_facet | A.G.A. Berto S.M. Oba Y.M. Michelacci L.O. Sampaio |
| author_sort | A.G.A. Berto |
| collection | DOAJ |
| description | In many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of chondroitin sulfate and tumor size, several individual specimens of human leiomyoma, a benign uterine tumor, were analyzed. The glycosaminoglycans from eight tumors were extracted and compared with those from the respective adjacent normal myometrium. The main glycosaminoglycan found in normal myometrium was dermatan sulfate, with small amounts of chondroitin sulfate and heparan sulfate. In leiomyoma, both dermatan sulfate and chondroitin sulfate were detected and the total amounts of the two galactosaminoglycans was increased in all tumors when compared to normal tissue. In contrast, the heparan sulfate concentration decreased in the tumor. To assess the disaccharide composition of galactosaminoglycans, these compounds were incubated with bacterial chondroitinases AC and ABC. The amounts of L-iduronic acid-containing disaccharides remained constant, whereas the concentration of D-glucuronic acid-containing disaccharides increased from 2 to 10 times in the tumor, indicating that D-glucuronic acid-containing disaccharides are responsible for the elevation in galactosaminoglycan concentration. This increase is positively correlated with tumor size. |
| first_indexed | 2024-12-14T09:53:10Z |
| format | Article |
| id | doaj.art-4b53303aaeb44f069fc3979229d5a1aa |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-14T09:53:10Z |
| publishDate | 2001-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-4b53303aaeb44f069fc3979229d5a1aa2022-12-21T23:07:28ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2001-05-0134563363710.1590/S0100-879X2001000500011Galactosaminoglycans from normal myometrium and leiomyomaA.G.A. BertoS.M. ObaY.M. MichelacciL.O. SampaioIn many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of chondroitin sulfate and tumor size, several individual specimens of human leiomyoma, a benign uterine tumor, were analyzed. The glycosaminoglycans from eight tumors were extracted and compared with those from the respective adjacent normal myometrium. The main glycosaminoglycan found in normal myometrium was dermatan sulfate, with small amounts of chondroitin sulfate and heparan sulfate. In leiomyoma, both dermatan sulfate and chondroitin sulfate were detected and the total amounts of the two galactosaminoglycans was increased in all tumors when compared to normal tissue. In contrast, the heparan sulfate concentration decreased in the tumor. To assess the disaccharide composition of galactosaminoglycans, these compounds were incubated with bacterial chondroitinases AC and ABC. The amounts of L-iduronic acid-containing disaccharides remained constant, whereas the concentration of D-glucuronic acid-containing disaccharides increased from 2 to 10 times in the tumor, indicating that D-glucuronic acid-containing disaccharides are responsible for the elevation in galactosaminoglycan concentration. This increase is positively correlated with tumor size.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500011galactosaminoglycanglycosaminoglycanchondroitin sulfatedermatan sulfateleiomyomamyometrium |
| spellingShingle | A.G.A. Berto S.M. Oba Y.M. Michelacci L.O. Sampaio Galactosaminoglycans from normal myometrium and leiomyoma Brazilian Journal of Medical and Biological Research galactosaminoglycan glycosaminoglycan chondroitin sulfate dermatan sulfate leiomyoma myometrium |
| title | Galactosaminoglycans from normal myometrium and leiomyoma |
| title_full | Galactosaminoglycans from normal myometrium and leiomyoma |
| title_fullStr | Galactosaminoglycans from normal myometrium and leiomyoma |
| title_full_unstemmed | Galactosaminoglycans from normal myometrium and leiomyoma |
| title_short | Galactosaminoglycans from normal myometrium and leiomyoma |
| title_sort | galactosaminoglycans from normal myometrium and leiomyoma |
| topic | galactosaminoglycan glycosaminoglycan chondroitin sulfate dermatan sulfate leiomyoma myometrium |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500011 |
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