E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel
E-p-methoxycinnamic acid (MCA) is one of the main active constituents of Scrophularia buergeriana Miquel and has hepatoprotective, anti-amnestic, and neuroprotective activities. For studying in vitro production of MCA, we established a hairy root culture of S. buergeriana by infecting leaf explants...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2010-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641003649P.pdf |
| _version_ | 1818473709279641600 |
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| author | Park Sang Un Li Xiaohua Eom Seok Hyun Lee Chung Yeol Lee Sook Young |
| author_facet | Park Sang Un Li Xiaohua Eom Seok Hyun Lee Chung Yeol Lee Sook Young |
| author_sort | Park Sang Un |
| collection | DOAJ |
| description | E-p-methoxycinnamic acid (MCA) is one of the main active constituents of Scrophularia buergeriana Miquel and has hepatoprotective, anti-amnestic, and neuroprotective activities. For studying in vitro production of MCA, we established a hairy root culture of S. buergeriana by infecting leaf explants with Agrobacterium rhizogenes R1000, and tested the growth and MCA production of these cultures using different strengths of media and concentrations of auxins. Hairy roots grown in half-strength MS medium showed the highest levels of growth (10.3 g/l) as well as MCA production (0.83 mg/100 g dry weight). Hairy root culture with the treatment of 0.5 mg/l IBA produced the highest amount of dry weight (11.8 g/l) and MCA (1.26 mg/100 g dry weight) production. These results demonstrate that the hairy root culture of S. buergeriana is a valuable alternative approach for the production of MCA. . |
| first_indexed | 2024-04-14T04:26:10Z |
| format | Article |
| id | doaj.art-4b54641acdf74126b6a34aec6358ad15 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-04-14T04:26:10Z |
| publishDate | 2010-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-4b54641acdf74126b6a34aec6358ad152022-12-22T02:12:17ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642010-01-0162364965210.2298/ABS1003649PE-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana MiquelPark Sang UnLi XiaohuaEom Seok HyunLee Chung YeolLee Sook YoungE-p-methoxycinnamic acid (MCA) is one of the main active constituents of Scrophularia buergeriana Miquel and has hepatoprotective, anti-amnestic, and neuroprotective activities. For studying in vitro production of MCA, we established a hairy root culture of S. buergeriana by infecting leaf explants with Agrobacterium rhizogenes R1000, and tested the growth and MCA production of these cultures using different strengths of media and concentrations of auxins. Hairy roots grown in half-strength MS medium showed the highest levels of growth (10.3 g/l) as well as MCA production (0.83 mg/100 g dry weight). Hairy root culture with the treatment of 0.5 mg/l IBA produced the highest amount of dry weight (11.8 g/l) and MCA (1.26 mg/100 g dry weight) production. These results demonstrate that the hairy root culture of S. buergeriana is a valuable alternative approach for the production of MCA. .http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641003649P.pdfAgrobacterium rhizogenesE-p-methoxycinnamic acid (MCA)hairy rootScrophularia buergeriana Miquel |
| spellingShingle | Park Sang Un Li Xiaohua Eom Seok Hyun Lee Chung Yeol Lee Sook Young E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel Archives of Biological Sciences Agrobacterium rhizogenes E-p-methoxycinnamic acid (MCA) hairy root Scrophularia buergeriana Miquel |
| title | E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel |
| title_full | E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel |
| title_fullStr | E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel |
| title_full_unstemmed | E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel |
| title_short | E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel |
| title_sort | e p methoxycinnamic acid production in hairy root cultures of scrophularia buergeriana miquel |
| topic | Agrobacterium rhizogenes E-p-methoxycinnamic acid (MCA) hairy root Scrophularia buergeriana Miquel |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641003649P.pdf |
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