GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max)
AbstractThe BABY BOOM (BBM) subfamily of the AP2/ERF transcription factor family is the main regulator of totipotency in plant cells and plays an important role in regulating cell proliferation and plant growth and development. Previously, we identified GmBBM7, a key gene of the soybean BBM gene sub...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2238833 |
| _version_ | 1797367467178196992 |
|---|---|
| author | Runnan Zhou Yujing Zhao Peng Cheng Binshuo Zhang Zhen Liu Sihui Wang Haibo Li Qingshan Chen Ying Zhao Sinan Li Xiaoxia Wu |
| author_facet | Runnan Zhou Yujing Zhao Peng Cheng Binshuo Zhang Zhen Liu Sihui Wang Haibo Li Qingshan Chen Ying Zhao Sinan Li Xiaoxia Wu |
| author_sort | Runnan Zhou |
| collection | DOAJ |
| description | AbstractThe BABY BOOM (BBM) subfamily of the AP2/ERF transcription factor family is the main regulator of totipotency in plant cells and plays an important role in regulating cell proliferation and plant growth and development. Previously, we identified GmBBM7, a key gene of the soybean BBM gene subfamily (GmBBM family). In this study, we aimed to analyze its molecular characteristics and role in somatic embryogenesis in soybean. First, we identified 17 members of GmBBM family. Phylogenetic and collinear analyses revealed that the GmBBM family genes mainly exhibited a strong genetic relationship with the those of PvBBM family, the BBM family of common bean (Phaseolus vulgaris). Analysis of the promoters revealed that GmBBM family genes were mainly regulated by plant hormones. The expression of GmBBM7 varied in various tissues of soybean. Particularly, it was higher in the roots, immature embryos, grains and callus. Subcellular localization analysis indicated that GmBBM7 encodes a nuclear protein. Furthermore, overexpressed GmBBM7 could significantly enhance callus formation by regulating the levels of gibberellin A1, A3 and A7; abscisic acid; and salicylic acid and could promote root growth and development. In summary, GmBBM7 is an important regulatory gene for somatic embryogenesis and root elongation in soybean. GmBBM7 overexpression in soybean could increase the callus formation rate and density through hormonal pathways and could increase root growth. This study provided theoretical basis for efficient breeding of soybean via gene editing, transgenic and other techniques. |
| first_indexed | 2024-03-08T17:18:45Z |
| format | Article |
| id | doaj.art-4b5c0fd784d64e04969cbe3e7676420f |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-03-08T17:18:45Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-4b5c0fd784d64e04969cbe3e7676420f2024-01-03T09:44:11ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-12-0137110.1080/13102818.2023.2238833GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max)Runnan Zhou0Yujing Zhao1Peng Cheng2Binshuo Zhang3Zhen Liu4Sihui Wang5Haibo Li6Qingshan Chen7Ying Zhao8Sinan Li9Xiaoxia Wu10Soybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaMaize National Engineering Lab, Maize Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, P. R. ChinaSoybean Genetic and Improvement Lab, College of Agriculture, Northeast Agricultural University, Harbin, Heilongjiang, P. R. ChinaAbstractThe BABY BOOM (BBM) subfamily of the AP2/ERF transcription factor family is the main regulator of totipotency in plant cells and plays an important role in regulating cell proliferation and plant growth and development. Previously, we identified GmBBM7, a key gene of the soybean BBM gene subfamily (GmBBM family). In this study, we aimed to analyze its molecular characteristics and role in somatic embryogenesis in soybean. First, we identified 17 members of GmBBM family. Phylogenetic and collinear analyses revealed that the GmBBM family genes mainly exhibited a strong genetic relationship with the those of PvBBM family, the BBM family of common bean (Phaseolus vulgaris). Analysis of the promoters revealed that GmBBM family genes were mainly regulated by plant hormones. The expression of GmBBM7 varied in various tissues of soybean. Particularly, it was higher in the roots, immature embryos, grains and callus. Subcellular localization analysis indicated that GmBBM7 encodes a nuclear protein. Furthermore, overexpressed GmBBM7 could significantly enhance callus formation by regulating the levels of gibberellin A1, A3 and A7; abscisic acid; and salicylic acid and could promote root growth and development. In summary, GmBBM7 is an important regulatory gene for somatic embryogenesis and root elongation in soybean. GmBBM7 overexpression in soybean could increase the callus formation rate and density through hormonal pathways and could increase root growth. This study provided theoretical basis for efficient breeding of soybean via gene editing, transgenic and other techniques.https://www.tandfonline.com/doi/10.1080/13102818.2023.2238833Glycine maxGmBBM7bioinformatics analysiscallushairy rootmolecular characteristics |
| spellingShingle | Runnan Zhou Yujing Zhao Peng Cheng Binshuo Zhang Zhen Liu Sihui Wang Haibo Li Qingshan Chen Ying Zhao Sinan Li Xiaoxia Wu GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max) Biotechnology & Biotechnological Equipment Glycine max GmBBM7 bioinformatics analysis callus hairy root molecular characteristics |
| title | GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max) |
| title_full | GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max) |
| title_fullStr | GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max) |
| title_full_unstemmed | GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max) |
| title_short | GmBBM7 promotes callus and root growth during somatic embryogenesis of soybean (Glycine max) |
| title_sort | gmbbm7 promotes callus and root growth during somatic embryogenesis of soybean glycine max |
| topic | Glycine max GmBBM7 bioinformatics analysis callus hairy root molecular characteristics |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2023.2238833 |
| work_keys_str_mv | AT runnanzhou gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT yujingzhao gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT pengcheng gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT binshuozhang gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT zhenliu gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT sihuiwang gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT haiboli gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT qingshanchen gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT yingzhao gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT sinanli gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax AT xiaoxiawu gmbbm7promotescallusandrootgrowthduringsomaticembryogenesisofsoybeanglycinemax |