Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challenges
Lameness is a serious concern in cows due to its multilevel effects and increasing incidences. The ability of anterior pituitary and hypothalamus to release LH, a prime reproductive hormone, is unknown in lame cows. Our study tested this ability by exogenous endocrine challenge using Buserelin aceta...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-09-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/93754 |
| _version_ | 1797896738337456128 |
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| author | PANKAJ SOOD A S NANDA A S NANDA B S PRAKASH |
| author_facet | PANKAJ SOOD A S NANDA A S NANDA B S PRAKASH |
| author_sort | PANKAJ SOOD |
| collection | DOAJ |
| description | Lameness is a serious concern in cows due to its multilevel effects and increasing incidences. The ability of anterior pituitary and hypothalamus to release LH, a prime reproductive hormone, is unknown in lame cows. Our study tested this ability by exogenous endocrine challenge using Buserelin acetate (GnRH analogue) and estradiol valerate (EV). The study included moderately lame (L; n=7) either cyclic (LC; n=3) or anestrus (LA; n=4); and normal cyclic, non–lame (NL; n=5) cows. Progesterone blocks LH release, so it was lowered by injecting PG in cyclic cows. After 14 h, all cows were given a walk for 5 min and immediately thereafter injected with 0.008 mg GnRH and 4 h later with 1 mg of EV. Blood plasma progesterone, cortisol and LH were quantified, periodically. There was a significant delay in the onset (105.0 vs 75.0±7.0 vs 54.0±9.0 min) and duration (22.5±5.3 vs 80.0±10.8 vs 117.0±2.7 min) of LH surge in LA vs LC vs NL cows; the surge was absent in two LA cows. The altered LH profile was attributed to a 3 fold (in L) vs 1.6 fold (in NL) rise in cortisol. LH released (ng/ml) in response to EV was also low in LA (31.5±2.4) and LC (69.9±7.2) than NL (79.2±7.0) cows. In conclusion, lame cows subjected to walk exhibited reduced pituitary response to release LH following exogenous GnRH as also a reduced ability of hypothalamus to stimulate LH release in response to exogenous estradiol. |
| first_indexed | 2024-04-10T07:46:31Z |
| format | Article |
| id | doaj.art-4b5c18750c8b44b2a9e90f2ef9bb1c52 |
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| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:46:31Z |
| publishDate | 2019-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-4b5c18750c8b44b2a9e90f2ef9bb1c522023-02-23T10:31:43ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-09-0189910.56093/ijans.v89i9.93754Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challengesPANKAJ SOOD0A S NANDA1A S NANDA2B S PRAKASH3Professor and Head, Department of Veterinary Clinical Complex, DGCN College of Veterinary and Animal Sciences, Palampur, Himachal PradeshVice Chancellor, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 IndiaPrincipal Scientist, Stress Physiology Lab, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, KarnatakaAdditional Director General (Former), ICAR, New Delhi.Lameness is a serious concern in cows due to its multilevel effects and increasing incidences. The ability of anterior pituitary and hypothalamus to release LH, a prime reproductive hormone, is unknown in lame cows. Our study tested this ability by exogenous endocrine challenge using Buserelin acetate (GnRH analogue) and estradiol valerate (EV). The study included moderately lame (L; n=7) either cyclic (LC; n=3) or anestrus (LA; n=4); and normal cyclic, non–lame (NL; n=5) cows. Progesterone blocks LH release, so it was lowered by injecting PG in cyclic cows. After 14 h, all cows were given a walk for 5 min and immediately thereafter injected with 0.008 mg GnRH and 4 h later with 1 mg of EV. Blood plasma progesterone, cortisol and LH were quantified, periodically. There was a significant delay in the onset (105.0 vs 75.0±7.0 vs 54.0±9.0 min) and duration (22.5±5.3 vs 80.0±10.8 vs 117.0±2.7 min) of LH surge in LA vs LC vs NL cows; the surge was absent in two LA cows. The altered LH profile was attributed to a 3 fold (in L) vs 1.6 fold (in NL) rise in cortisol. LH released (ng/ml) in response to EV was also low in LA (31.5±2.4) and LC (69.9±7.2) than NL (79.2±7.0) cows. In conclusion, lame cows subjected to walk exhibited reduced pituitary response to release LH following exogenous GnRH as also a reduced ability of hypothalamus to stimulate LH release in response to exogenous estradiol.https://epubs.icar.org.in/index.php/IJAnS/article/view/93754CowsEndocrine challengeExogenousLamenessLH production |
| spellingShingle | PANKAJ SOOD A S NANDA A S NANDA B S PRAKASH Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challenges Indian Journal of Animal Sciences Cows Endocrine challenge Exogenous Lameness LH production |
| title | Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challenges |
| title_full | Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challenges |
| title_fullStr | Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challenges |
| title_full_unstemmed | Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challenges |
| title_short | Lame cows have a subdued hypothalamus-pituitary axis as revealed by LH release in response to exogenous endocrine challenges |
| title_sort | lame cows have a subdued hypothalamus pituitary axis as revealed by lh release in response to exogenous endocrine challenges |
| topic | Cows Endocrine challenge Exogenous Lameness LH production |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/93754 |
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