Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model
Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2016-11-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/2632.pdf |
| _version_ | 1827606988538773504 |
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| author | Tobias C. Wood Camilla Simmons Samuel A. Hurley Anthony C. Vernon Joel Torres Flavio Dell’Acqua Steve C.R. Williams Diana Cash |
| author_facet | Tobias C. Wood Camilla Simmons Samuel A. Hurley Anthony C. Vernon Joel Torres Flavio Dell’Acqua Steve C.R. Williams Diana Cash |
| author_sort | Tobias C. Wood |
| collection | DOAJ |
| description | Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component relaxometry, Diffusion Tensor Imaging (DTI) and morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. The presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model. |
| first_indexed | 2024-03-09T06:47:07Z |
| format | Article |
| id | doaj.art-4b5d0fc9eee34d06bb63820273118315 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:47:07Z |
| publishDate | 2016-11-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-4b5d0fc9eee34d06bb638202731183152023-12-03T10:33:30ZengPeerJ Inc.PeerJ2167-83592016-11-014e263210.7717/peerj.2632Whole-brain ex-vivo quantitative MRI of the cuprizone mouse modelTobias C. Wood0Camilla Simmons1Samuel A. Hurley2Anthony C. Vernon3Joel Torres4Flavio Dell’Acqua5Steve C.R. Williams6Diana Cash7Department of Neuroimaging, IOPPN, King’s College London, London, United KingdomDepartment of Neuroimaging, IOPPN, King’s College London, London, United KingdomFMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, Oxfordshire, United KingdomCells and Behaviour Unit, Department of Basic and Clinical Neuroscience, IOPPN, King’s College London, London, United KingdomDepartment of Neuroimaging, IOPPN, King’s College London, London, United KingdomDepartment of Neuroimaging, IOPPN, King’s College London, London, United KingdomDepartment of Neuroimaging, IOPPN, King’s College London, London, United KingdomDepartment of Neuroimaging, IOPPN, King’s College London, London, United KingdomMyelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component relaxometry, Diffusion Tensor Imaging (DTI) and morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. The presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model.https://peerj.com/articles/2632.pdfCuprizoneMyelinQuantitative imagingMRIInflammationMouse |
| spellingShingle | Tobias C. Wood Camilla Simmons Samuel A. Hurley Anthony C. Vernon Joel Torres Flavio Dell’Acqua Steve C.R. Williams Diana Cash Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model PeerJ Cuprizone Myelin Quantitative imaging MRI Inflammation Mouse |
| title | Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model |
| title_full | Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model |
| title_fullStr | Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model |
| title_full_unstemmed | Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model |
| title_short | Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model |
| title_sort | whole brain ex vivo quantitative mri of the cuprizone mouse model |
| topic | Cuprizone Myelin Quantitative imaging MRI Inflammation Mouse |
| url | https://peerj.com/articles/2632.pdf |
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