Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography
A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column a...
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| Format: | Article |
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MDPI AG
2019-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/8/4/226 |
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| author | Juan Peris-Vicente Daniel García-Ferrer Pooja Mishra Jaume Albiol-Chiva Abhilasha Durgbanshi Samuel Carda-Broch Devasish Bose Josep Esteve-Romero |
| author_facet | Juan Peris-Vicente Daniel García-Ferrer Pooja Mishra Jaume Albiol-Chiva Abhilasha Durgbanshi Samuel Carda-Broch Devasish Bose Josep Esteve-Romero |
| author_sort | Juan Peris-Vicente |
| collection | DOAJ |
| description | A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (<i>R</i><sup>2</sup> > 0.9991), limit of detection and decision limit (0.01–0.05 mg/kg), limit of quantification (0.025–0.150 mg/kg), detection capability (<0.4 times decision limit), trueness (−14.2% to +9.8%), precision (<14.0%), robustness, and stability. The procedure was environmentally friendly, safe, easy-to-conduct, inexpensive, and had a high sample throughput, thus it is useful for routine analysis as a screening method in a laboratory for food residue control. |
| first_indexed | 2024-12-19T16:52:42Z |
| format | Article |
| id | doaj.art-4b611c8d22dc4e86a7e96ee9a30794ea |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-19T16:52:42Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-4b611c8d22dc4e86a7e96ee9a30794ea2022-12-21T20:13:29ZengMDPI AGAntibiotics2079-63822019-11-018422610.3390/antibiotics8040226antibiotics8040226Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid ChromatographyJuan Peris-Vicente0Daniel García-Ferrer1Pooja Mishra2Jaume Albiol-Chiva3Abhilasha Durgbanshi4Samuel Carda-Broch5Devasish Bose6Josep Esteve-Romero7Department of Analytical Chemistry, Faculty of Chemistry, Universitat de València, 46100 Burjassot, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, Universitat de València, 46100 Burjassot, SpainDepartment of Chemistry, Doctor Harisingh Gour Vishwavidyalaya (A Central University), Madhya Pradesh 470003, IndiaBioanalytical Chemistrya, Department of Physical and Analytical Chemistrya, ESTCE, Universitat Jaume I, 12071 Castelló, SpainDepartment of Chemistry, Doctor Harisingh Gour Vishwavidyalaya (A Central University), Madhya Pradesh 470003, IndiaBioanalytical Chemistrya, Department of Physical and Analytical Chemistrya, ESTCE, Universitat Jaume I, 12071 Castelló, SpainDepartment of Criminology and Forensic Science, Doctor Harisingh Gour Vishwavidyalaya (A Central University), Madhya Pradesh 470003, IndiaBioanalytical Chemistrya, Department of Physical and Analytical Chemistrya, ESTCE, Universitat Jaume I, 12071 Castelló, SpainA method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (<i>R</i><sup>2</sup> > 0.9991), limit of detection and decision limit (0.01–0.05 mg/kg), limit of quantification (0.025–0.150 mg/kg), detection capability (<0.4 times decision limit), trueness (−14.2% to +9.8%), precision (<14.0%), robustness, and stability. The procedure was environmentally friendly, safe, easy-to-conduct, inexpensive, and had a high sample throughput, thus it is useful for routine analysis as a screening method in a laboratory for food residue control.https://www.mdpi.com/2079-6382/8/4/226food safetyfluorescencelaying henoptimizationsolid-to-liquid extractionvalidation |
| spellingShingle | Juan Peris-Vicente Daniel García-Ferrer Pooja Mishra Jaume Albiol-Chiva Abhilasha Durgbanshi Samuel Carda-Broch Devasish Bose Josep Esteve-Romero Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography Antibiotics food safety fluorescence laying hen optimization solid-to-liquid extraction validation |
| title | Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography |
| title_full | Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography |
| title_fullStr | Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography |
| title_full_unstemmed | Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography |
| title_short | Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography |
| title_sort | procedure for the screening of eggs and egg products to detect oxolonic acid ciprofloxacin enrofloxacin and sarafloxacin using micellar liquid chromatography |
| topic | food safety fluorescence laying hen optimization solid-to-liquid extraction validation |
| url | https://www.mdpi.com/2079-6382/8/4/226 |
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