Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in Finland
Abstract Campylobacter spp., especially C. jejuni, is the most common zoonotic pathogen in humans worldwide. In Nordic countries, the prevalence of C. jejuni in broilers, which is an important reservoir of human infections, is generally low. Thus, other sources of domestically acquired infections be...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13028-023-00698-7 |
| _version_ | 1797752612743806976 |
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| author | Rauni Kivistö Mikaela Sauvala Maria Fredriksson-Ahomaa Johanna Björkroth |
| author_facet | Rauni Kivistö Mikaela Sauvala Maria Fredriksson-Ahomaa Johanna Björkroth |
| author_sort | Rauni Kivistö |
| collection | DOAJ |
| description | Abstract Campylobacter spp., especially C. jejuni, is the most common zoonotic pathogen in humans worldwide. In Nordic countries, the prevalence of C. jejuni in broilers, which is an important reservoir of human infections, is generally low. Thus, other sources of domestically acquired infections besides chicken meat need to be considered. Game birds are known to carry a variety of zoonotic agents, including Campylobacter. The aim of this study was to investigate Campylobacter spp. carriage in a flock of reared pheasants at hunting in two successive samplings to better understand the dynamics of Campylobacter infections in pheasants. Overall, 72% of the intestinal samples were positive for Campylobacter spp. by direct culture on mCCDA. C. jejuni was the only species identified. The isolates were genotyped using whole genome sequencing (WGS), multilocus sequence typing (MLST), and ad hoc whole genome MLST (wgMLST). Two distinct C. jejuni clones were identified among the 18 isolates studied, representing MLST sequence types (STs) ST-45 and ST-699. The ST-45 isolates were closely related to previous human clinical isolates using core genome MLST (cgMLST). In contrast, the ST-699 isolates forming the dominant clone in the latter sampling were quite distinct from previously described cgMLST profiles from different hosts and sources worldwide. In conclusion, the intestine of reared pheasants is commonly colonized by C. jejuni and may carry genotypes relevant to infections in livestock and humans. Hygienic measures are needed to limit the spread of infection in reared flocks. Especially farmers and hunters having direct contact with pheasant offal need to be aware of the associated zoonosis risk to protect themselves and their working dogs alike. Biosecurity measures to improve the safety and reduce the zoonosis risk associated with pheasant farming should be further investigated. |
| first_indexed | 2024-03-12T17:07:00Z |
| format | Article |
| id | doaj.art-4b6374b88c0140c98943576ea8a5844f |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-03-12T17:07:00Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-4b6374b88c0140c98943576ea8a5844f2023-08-06T11:21:15ZengBMCActa Veterinaria Scandinavica1751-01472023-08-016511510.1186/s13028-023-00698-7Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in FinlandRauni Kivistö0Mikaela Sauvala1Maria Fredriksson-Ahomaa2Johanna Björkroth3Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of HelsinkiDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of HelsinkiDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of HelsinkiDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of HelsinkiAbstract Campylobacter spp., especially C. jejuni, is the most common zoonotic pathogen in humans worldwide. In Nordic countries, the prevalence of C. jejuni in broilers, which is an important reservoir of human infections, is generally low. Thus, other sources of domestically acquired infections besides chicken meat need to be considered. Game birds are known to carry a variety of zoonotic agents, including Campylobacter. The aim of this study was to investigate Campylobacter spp. carriage in a flock of reared pheasants at hunting in two successive samplings to better understand the dynamics of Campylobacter infections in pheasants. Overall, 72% of the intestinal samples were positive for Campylobacter spp. by direct culture on mCCDA. C. jejuni was the only species identified. The isolates were genotyped using whole genome sequencing (WGS), multilocus sequence typing (MLST), and ad hoc whole genome MLST (wgMLST). Two distinct C. jejuni clones were identified among the 18 isolates studied, representing MLST sequence types (STs) ST-45 and ST-699. The ST-45 isolates were closely related to previous human clinical isolates using core genome MLST (cgMLST). In contrast, the ST-699 isolates forming the dominant clone in the latter sampling were quite distinct from previously described cgMLST profiles from different hosts and sources worldwide. In conclusion, the intestine of reared pheasants is commonly colonized by C. jejuni and may carry genotypes relevant to infections in livestock and humans. Hygienic measures are needed to limit the spread of infection in reared flocks. Especially farmers and hunters having direct contact with pheasant offal need to be aware of the associated zoonosis risk to protect themselves and their working dogs alike. Biosecurity measures to improve the safety and reduce the zoonosis risk associated with pheasant farming should be further investigated.https://doi.org/10.1186/s13028-023-00698-7CampylobacteriosisC. jejuniGame birdHuntingOne HealthPathogen |
| spellingShingle | Rauni Kivistö Mikaela Sauvala Maria Fredriksson-Ahomaa Johanna Björkroth Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in Finland Acta Veterinaria Scandinavica Campylobacteriosis C. jejuni Game bird Hunting One Health Pathogen |
| title | Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in Finland |
| title_full | Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in Finland |
| title_fullStr | Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in Finland |
| title_full_unstemmed | Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in Finland |
| title_short | Prevalence and genotype diversity of Campylobacter jejuni in hunted reared pheasants (Phasianus colchicus) in Finland |
| title_sort | prevalence and genotype diversity of campylobacter jejuni in hunted reared pheasants phasianus colchicus in finland |
| topic | Campylobacteriosis C. jejuni Game bird Hunting One Health Pathogen |
| url | https://doi.org/10.1186/s13028-023-00698-7 |
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