Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiota
Background and Aim: The gut microbiome plays an important role in the overall health and well-being of dogs, influencing various physiological processes such as metabolism, nutrient absorption, and immune function. Edible insects are a sustainable and nutritious alternative protein source attracting...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-08-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.16/August-2023/7.pdf |
| _version_ | 1797741257588473856 |
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| author | Sathita Areerat Pipatpong Chundang Chalermpol Lekcharoensuk Preecha Patumcharoenpol Attawit Kovitvadhi |
| author_facet | Sathita Areerat Pipatpong Chundang Chalermpol Lekcharoensuk Preecha Patumcharoenpol Attawit Kovitvadhi |
| author_sort | Sathita Areerat |
| collection | DOAJ |
| description | Background and Aim: The gut microbiome plays an important role in the overall health and well-being of dogs, influencing various physiological processes such as metabolism, nutrient absorption, and immune function. Edible insects are a sustainable and nutritious alternative protein source attracting increasing attention as a potential component of animal feeds, including pet food. However, little is known about the effects of insect-based diets on the gut microbiota of dogs. This study aimed to examine the fecal microbiota of dogs fed a diet that substituted common protein sources (poultry meal) with the house cricket (Acheta domesticus [AD]) or mulberry silkworm pupae (Bombyx mori pupae [BMp]) at different levels.
Materials and Methods: Fifteen healthy adult mixed-breed dogs were systemically randomized and assigned into each block under a completed randomized block design into the following five experimental dietary groups: control diet, 10% AD, 20% AD, 7% BMp, or 14% BMp for 29 days. The amounts fed to the dogs were based on the daily energy requirement. Fecal samples were collected on days 14 and 29 and analyzed for bacterial community structure using 16S ribosomal ribonucleic acid gene sequencing.
Results: At the phylum and genus levels, microbiota and their diversity were generally relatively similar among all treatments. The diets containing insects did not significantly alter the major phyla in the gut microbiome of dogs (p > 0.05). A few significant changes were found in the relative abundance of bacterial genera, with the levels of Allobaculum and Turicibacter being reduced in dogs fed a higher level of BMp. In contrast, only a decrease in Turicibacter was found in dogs fed the lower level of AD than the control diet (p < 0.05). Corynebacterium and Lactobacillus levels in the dogs fed 14% BMp were significantly increased compared with those in the control group (p < 0.05).
Conclusion: These findings suggest that insect-based diets may slightly alter the gut microbiota of dogs. Further research is needed to fully understand the mechanisms by which insect-based diets influence the gut microbiota of dogs and the long-term potential health implications. |
| first_indexed | 2024-03-12T14:24:13Z |
| format | Article |
| id | doaj.art-4b64cef6a6f746218229b98bdd665811 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-12T14:24:13Z |
| publishDate | 2023-08-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-4b64cef6a6f746218229b98bdd6658112023-08-18T11:41:57ZengVeterinary WorldVeterinary World0972-89882231-09162023-08-011681627163510.14202/vetworld.2023.1627-1635Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiotaSathita Areerat0https://orcid.org/0000-0002-3174-8471Pipatpong Chundang1https://orcid.org/0000-0003-0159-0869Chalermpol Lekcharoensuk2https://orcid.org/0000-0001-8540-1695Preecha Patumcharoenpol3https://orcid.org/0000-0002-5186-8577Attawit Kovitvadhi4https://orcid.org/0000-0002-0769-5234 Graduate Student in Animal Health and Biomedical Science Program, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand. Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand. Department of Companion Animals Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand. Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.Background and Aim: The gut microbiome plays an important role in the overall health and well-being of dogs, influencing various physiological processes such as metabolism, nutrient absorption, and immune function. Edible insects are a sustainable and nutritious alternative protein source attracting increasing attention as a potential component of animal feeds, including pet food. However, little is known about the effects of insect-based diets on the gut microbiota of dogs. This study aimed to examine the fecal microbiota of dogs fed a diet that substituted common protein sources (poultry meal) with the house cricket (Acheta domesticus [AD]) or mulberry silkworm pupae (Bombyx mori pupae [BMp]) at different levels. Materials and Methods: Fifteen healthy adult mixed-breed dogs were systemically randomized and assigned into each block under a completed randomized block design into the following five experimental dietary groups: control diet, 10% AD, 20% AD, 7% BMp, or 14% BMp for 29 days. The amounts fed to the dogs were based on the daily energy requirement. Fecal samples were collected on days 14 and 29 and analyzed for bacterial community structure using 16S ribosomal ribonucleic acid gene sequencing. Results: At the phylum and genus levels, microbiota and their diversity were generally relatively similar among all treatments. The diets containing insects did not significantly alter the major phyla in the gut microbiome of dogs (p > 0.05). A few significant changes were found in the relative abundance of bacterial genera, with the levels of Allobaculum and Turicibacter being reduced in dogs fed a higher level of BMp. In contrast, only a decrease in Turicibacter was found in dogs fed the lower level of AD than the control diet (p < 0.05). Corynebacterium and Lactobacillus levels in the dogs fed 14% BMp were significantly increased compared with those in the control group (p < 0.05). Conclusion: These findings suggest that insect-based diets may slightly alter the gut microbiota of dogs. Further research is needed to fully understand the mechanisms by which insect-based diets influence the gut microbiota of dogs and the long-term potential health implications.https://www.veterinaryworld.org/Vol.16/August-2023/7.pdf16s ribosomal ribonucleic acidcaninecricketgut microbiotainsectsilkworm. |
| spellingShingle | Sathita Areerat Pipatpong Chundang Chalermpol Lekcharoensuk Preecha Patumcharoenpol Attawit Kovitvadhi Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiota Veterinary World 16s ribosomal ribonucleic acid canine cricket gut microbiota insect silkworm. |
| title | Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiota |
| title_full | Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiota |
| title_fullStr | Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiota |
| title_full_unstemmed | Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiota |
| title_short | Insect-based diets (house crickets and mulberry silkworm pupae): A comparison of their effects on canine gut microbiota |
| title_sort | insect based diets house crickets and mulberry silkworm pupae a comparison of their effects on canine gut microbiota |
| topic | 16s ribosomal ribonucleic acid canine cricket gut microbiota insect silkworm. |
| url | https://www.veterinaryworld.org/Vol.16/August-2023/7.pdf |
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