iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties
Most pears in Anhui Province are a kind of self-incompatible fruit whose quality is strongly influenced by the male pollen. The proteomic variation of Dangshan Su pollinated by different varieties was analysed using the isobaric tag for relative and absolute quantitation (iTRAQ) to investigate the e...
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MDPI AG
2018-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/3/548 |
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| author | Shumei Li Xueqiang Su Qing Jin Guohui Li Yanming Sun Muhammad Abdullah Yongping Cai Yi Lin |
| author_facet | Shumei Li Xueqiang Su Qing Jin Guohui Li Yanming Sun Muhammad Abdullah Yongping Cai Yi Lin |
| author_sort | Shumei Li |
| collection | DOAJ |
| description | Most pears in Anhui Province are a kind of self-incompatible fruit whose quality is strongly influenced by the male pollen. The proteomic variation of Dangshan Su pollinated by different varieties was analysed using the isobaric tag for relative and absolute quantitation (iTRAQ) to investigate the effect of pollination by different varieties on the pear lignin pathway. Among the 3980 proteins identified from the two samples, 139 proteins were identified as differentially expressed proteins (DEPs). Of these proteins, laccase-4 (LAC4), was found to be related with lignin synthesis, and β-glucosidase 15 (BGLU15) and peroxidase 47 (PER47) were involved in the phenylpropanoid pathway. Moreover, the lignin and stone cell contents were lower in DW (Dangshan Su pollinated by Wonhwang) than those in DJ (Dangshan Su pollinated by Jingbaili). The effect of pollination on the synthesis of lignin through the regulation of the expression of PER47, BGLU15 and LAC4 ultimately affects the formation of stone cells and the fruit quality. We report for the first time that different pollinations influence the protein expression profile in the Dangshan Su pear, and this result provides some new epididymal targets for regulating the synthesis of lignin, regulating the content of stone cells and improving the quality of the pears. |
| first_indexed | 2024-12-10T13:07:02Z |
| format | Article |
| id | doaj.art-4b6eb77786d54579ad6cec3a53fd4590 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T13:07:02Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4b6eb77786d54579ad6cec3a53fd45902022-12-22T01:47:48ZengMDPI AGMolecules1420-30492018-03-0123354810.3390/molecules23030548molecules23030548iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different VarietiesShumei Li0Xueqiang Su1Qing Jin2Guohui Li3Yanming Sun4Muhammad Abdullah5Yongping Cai6Yi Lin7School of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaSchool of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaSchool of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaSchool of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaSchool of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaSchool of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaSchool of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaSchool of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, ChinaMost pears in Anhui Province are a kind of self-incompatible fruit whose quality is strongly influenced by the male pollen. The proteomic variation of Dangshan Su pollinated by different varieties was analysed using the isobaric tag for relative and absolute quantitation (iTRAQ) to investigate the effect of pollination by different varieties on the pear lignin pathway. Among the 3980 proteins identified from the two samples, 139 proteins were identified as differentially expressed proteins (DEPs). Of these proteins, laccase-4 (LAC4), was found to be related with lignin synthesis, and β-glucosidase 15 (BGLU15) and peroxidase 47 (PER47) were involved in the phenylpropanoid pathway. Moreover, the lignin and stone cell contents were lower in DW (Dangshan Su pollinated by Wonhwang) than those in DJ (Dangshan Su pollinated by Jingbaili). The effect of pollination on the synthesis of lignin through the regulation of the expression of PER47, BGLU15 and LAC4 ultimately affects the formation of stone cells and the fruit quality. We report for the first time that different pollinations influence the protein expression profile in the Dangshan Su pear, and this result provides some new epididymal targets for regulating the synthesis of lignin, regulating the content of stone cells and improving the quality of the pears.http://www.mdpi.com/1420-3049/23/3/548Dangshan Su peariTRAQdifferent pollinationsproteomicligninphenylpropanoid pathway |
| spellingShingle | Shumei Li Xueqiang Su Qing Jin Guohui Li Yanming Sun Muhammad Abdullah Yongping Cai Yi Lin iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties Molecules Dangshan Su pear iTRAQ different pollinations proteomic lignin phenylpropanoid pathway |
| title | iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties |
| title_full | iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties |
| title_fullStr | iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties |
| title_full_unstemmed | iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties |
| title_short | iTRAQ-Based Identification of Proteins Related to Lignin Synthesis in the Pear Pollinated with Pollen from Different Varieties |
| title_sort | itraq based identification of proteins related to lignin synthesis in the pear pollinated with pollen from different varieties |
| topic | Dangshan Su pear iTRAQ different pollinations proteomic lignin phenylpropanoid pathway |
| url | http://www.mdpi.com/1420-3049/23/3/548 |
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