Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation
Three calmodulin (PvCaM-1, PvCaM-2, and PvCaM-3) clones were isolated from a Phaseolus vulgaris nodule cDNA library. All clones contain the complete coding region and are 62 to 74% homologous within this region. Compared to plant CaM consensus sequences, PvCaM-2 has a novel tyrosine118 residue, repr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2002-05-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.5.428 |
| _version_ | 1818914925595066368 |
|---|---|
| author | Alberto Camas Luis Cárdenas Carmen Quinto Miguel Lara |
| author_facet | Alberto Camas Luis Cárdenas Carmen Quinto Miguel Lara |
| author_sort | Alberto Camas |
| collection | DOAJ |
| description | Three calmodulin (PvCaM-1, PvCaM-2, and PvCaM-3) clones were isolated from a Phaseolus vulgaris nodule cDNA library. All clones contain the complete coding region and are 62 to 74% homologous within this region. Compared to plant CaM consensus sequences, PvCaM-2 has a novel tyrosine118 residue, representing a putative phosphorylation site. Southern analysis suggested that calmodulin is encoded by a gene family. These three CaM clones are expressed mainly in young tissues and meristems. The expression pattern of PvCaM-2 and PvCaM-3 is almost identical but different from that of PvCaM-1, suggesting that PvCaM-1 is a well-defined CaM gene, whereas PvCaM-2 and PvCaM-3 could be alleles. PvCaM clones are expressed early in nodules, and transcript levels increase from nodule primordia to nodule-like structures induced by the Nod factor. Conversely, in roots, Nod factor lowers mRNA levels of all three PvCaM clones, but especially of PvCaM-1. Inhibition of PvCaM-1 expression also is observed when 2,3,5-triiodobenzoic acid is added and is prevented when roots are treated with indole-3-acetic acid, suggesting that PvCaM-1 regulation is related to the Nod factor inhibition of polar auxin transport. These results could suggest that CaM clones do not participate in the early signaling generated by the Nod factor but do participate in early events of nodule formation. |
| first_indexed | 2024-12-19T23:54:08Z |
| format | Article |
| id | doaj.art-4b6f434d279b479b9333efc3e4b6c9ee |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:54:08Z |
| publishDate | 2002-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-4b6f434d279b479b9333efc3e4b6c9ee2022-12-21T20:01:03ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062002-05-0115542843610.1094/MPMI.2002.15.5.428Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene RegulationAlberto CamasLuis CárdenasCarmen QuintoMiguel LaraThree calmodulin (PvCaM-1, PvCaM-2, and PvCaM-3) clones were isolated from a Phaseolus vulgaris nodule cDNA library. All clones contain the complete coding region and are 62 to 74% homologous within this region. Compared to plant CaM consensus sequences, PvCaM-2 has a novel tyrosine118 residue, representing a putative phosphorylation site. Southern analysis suggested that calmodulin is encoded by a gene family. These three CaM clones are expressed mainly in young tissues and meristems. The expression pattern of PvCaM-2 and PvCaM-3 is almost identical but different from that of PvCaM-1, suggesting that PvCaM-1 is a well-defined CaM gene, whereas PvCaM-2 and PvCaM-3 could be alleles. PvCaM clones are expressed early in nodules, and transcript levels increase from nodule primordia to nodule-like structures induced by the Nod factor. Conversely, in roots, Nod factor lowers mRNA levels of all three PvCaM clones, but especially of PvCaM-1. Inhibition of PvCaM-1 expression also is observed when 2,3,5-triiodobenzoic acid is added and is prevented when roots are treated with indole-3-acetic acid, suggesting that PvCaM-1 regulation is related to the Nod factor inhibition of polar auxin transport. These results could suggest that CaM clones do not participate in the early signaling generated by the Nod factor but do participate in early events of nodule formation.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.5.428auxin transport inhibitionlipochitooligosaccharideRhizobium etli |
| spellingShingle | Alberto Camas Luis Cárdenas Carmen Quinto Miguel Lara Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation Molecular Plant-Microbe Interactions auxin transport inhibition lipochitooligosaccharide Rhizobium etli |
| title | Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation |
| title_full | Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation |
| title_fullStr | Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation |
| title_full_unstemmed | Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation |
| title_short | Expression of Different Calmodulin Genes in Bean (Phaseolus vulgaris L.): Role of Nod Factor on Calmodulin Gene Regulation |
| title_sort | expression of different calmodulin genes in bean phaseolus vulgaris l role of nod factor on calmodulin gene regulation |
| topic | auxin transport inhibition lipochitooligosaccharide Rhizobium etli |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.5.428 |
| work_keys_str_mv | AT albertocamas expressionofdifferentcalmodulingenesinbeanphaseolusvulgarislroleofnodfactoroncalmodulingeneregulation AT luiscardenas expressionofdifferentcalmodulingenesinbeanphaseolusvulgarislroleofnodfactoroncalmodulingeneregulation AT carmenquinto expressionofdifferentcalmodulingenesinbeanphaseolusvulgarislroleofnodfactoroncalmodulingeneregulation AT miguellara expressionofdifferentcalmodulingenesinbeanphaseolusvulgarislroleofnodfactoroncalmodulingeneregulation |