Seasonal variations of cauda epididymal spermatozoa of bucks
Objective: The study was conducted to evaluate the influence of season on cauda epididymal spermatozoa isolated from bucks. Materials and methods: Testes of 30 mature bucks were collected from local slaughter house, and were processed for the retrieval of cauda epididymal spermatozoa for evaluation...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2016-09-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://www.scopemed.org/fulltextpdf.php?mno=227795 |
| _version_ | 1818935536482516992 |
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| author | Dilip Kumar Swain Sarvajeet Yadav Vijay Pandey |
| author_facet | Dilip Kumar Swain Sarvajeet Yadav Vijay Pandey |
| author_sort | Dilip Kumar Swain |
| collection | DOAJ |
| description | Objective: The study was conducted to evaluate the influence of season on cauda epididymal spermatozoa isolated from bucks.
Materials and methods: Testes of 30 mature bucks were collected from local slaughter house, and were processed for the retrieval of cauda epididymal spermatozoa for evaluation. Testes were collected in three seasons (winter, summer and rainy), and each season was having 10 pairs of testicles. Recovered spermatozoa from the cauda epididymis were processed immediately for evaluation of semen attributes (Spermatozoa motility, viability, plasma membrane integrity, acrosomal status and DNA integrity).
Results: Physiological effect of season was observed on progressive motility, percent of live spermatozoa, spermatozoal membrane integrity (HOST), acrosomal integrity, capacitation status and DNA integrity. Progressive motility, percent live spermatozoa, HOST positive spermatozoa, were found significantly (P<0.05) high in summer season, whereas, significantly (P<0.05) lower comet positive spermatozoa were found in summer season as compared to rainy and winter. Compromised acrosomal status was seen in winter and rainy seasons as compared to summer.
Conclusion: Compromised acrosome along with plasma membrane and higher percentage of spermatozoa with damaged DNA in cauda spermatozoa were observed during winter and rainy seasons as compared to summer season. Summer season was found to be the most suitable season for collection of cauda epididymal spermatozoa and can effectively be used for assisted reproduction with further investigations of associated mechanisms. [J Adv Vet Anim Res 2016; 3(3.000): 263-267] |
| first_indexed | 2024-12-20T05:21:44Z |
| format | Article |
| id | doaj.art-4b74ba24b38d4ce3b86491ce572bcc6c |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-20T05:21:44Z |
| publishDate | 2016-09-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-4b74ba24b38d4ce3b86491ce572bcc6c2022-12-21T19:52:00ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102016-09-013326326710.5455/javar.2016.c155227795Seasonal variations of cauda epididymal spermatozoa of bucksDilip Kumar Swain0Sarvajeet Yadav1Vijay Pandey2Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh, INDIA Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh, INDIA Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh, INDIAObjective: The study was conducted to evaluate the influence of season on cauda epididymal spermatozoa isolated from bucks. Materials and methods: Testes of 30 mature bucks were collected from local slaughter house, and were processed for the retrieval of cauda epididymal spermatozoa for evaluation. Testes were collected in three seasons (winter, summer and rainy), and each season was having 10 pairs of testicles. Recovered spermatozoa from the cauda epididymis were processed immediately for evaluation of semen attributes (Spermatozoa motility, viability, plasma membrane integrity, acrosomal status and DNA integrity). Results: Physiological effect of season was observed on progressive motility, percent of live spermatozoa, spermatozoal membrane integrity (HOST), acrosomal integrity, capacitation status and DNA integrity. Progressive motility, percent live spermatozoa, HOST positive spermatozoa, were found significantly (P<0.05) high in summer season, whereas, significantly (P<0.05) lower comet positive spermatozoa were found in summer season as compared to rainy and winter. Compromised acrosomal status was seen in winter and rainy seasons as compared to summer. Conclusion: Compromised acrosome along with plasma membrane and higher percentage of spermatozoa with damaged DNA in cauda spermatozoa were observed during winter and rainy seasons as compared to summer season. Summer season was found to be the most suitable season for collection of cauda epididymal spermatozoa and can effectively be used for assisted reproduction with further investigations of associated mechanisms. [J Adv Vet Anim Res 2016; 3(3.000): 263-267]http://www.scopemed.org/fulltextpdf.php?mno=227795Cauda epididymisComet assayDNA integrityHOSTSeasonSpermatozoa attributes |
| spellingShingle | Dilip Kumar Swain Sarvajeet Yadav Vijay Pandey Seasonal variations of cauda epididymal spermatozoa of bucks Journal of Advanced Veterinary and Animal Research Cauda epididymis Comet assay DNA integrity HOST Season Spermatozoa attributes |
| title | Seasonal variations of cauda epididymal spermatozoa of bucks |
| title_full | Seasonal variations of cauda epididymal spermatozoa of bucks |
| title_fullStr | Seasonal variations of cauda epididymal spermatozoa of bucks |
| title_full_unstemmed | Seasonal variations of cauda epididymal spermatozoa of bucks |
| title_short | Seasonal variations of cauda epididymal spermatozoa of bucks |
| title_sort | seasonal variations of cauda epididymal spermatozoa of bucks |
| topic | Cauda epididymis Comet assay DNA integrity HOST Season Spermatozoa attributes |
| url | http://www.scopemed.org/fulltextpdf.php?mno=227795 |
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