Diversity and assembly of root-associated microbiomes of rubber trees
IntroductionUnderstanding the diversity and assembly of the microbiomes of plant roots is crucial to manipulate them for sustainable ecosystem functioning. However, there are few reports about microbial communities at a continuous fine-scale of roots for rubber trees.MethodsWe investigate the struct...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136418/full |
| _version_ | 1827975748263084032 |
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| author | Guoyu Lan Guoyu Lan Yaqing Wei Yaqing Wei Yuwu Li Zhixiang Wu Zhixiang Wu |
| author_facet | Guoyu Lan Guoyu Lan Yaqing Wei Yaqing Wei Yuwu Li Zhixiang Wu Zhixiang Wu |
| author_sort | Guoyu Lan |
| collection | DOAJ |
| description | IntroductionUnderstanding the diversity and assembly of the microbiomes of plant roots is crucial to manipulate them for sustainable ecosystem functioning. However, there are few reports about microbial communities at a continuous fine-scale of roots for rubber trees.MethodsWe investigate the structure, diversity, and assembly of bacterial and fungal communities for the soil (non-rhizosphere), rhizosphere, and rhizoplane as well as root endosphere of rubber trees using the amplicon sequencing of 16S ribosomal ribonucleic acid (rRNA) and Internally Transcribed Spacer (ITS) genes.ResultsWe show that 18.69% of bacterial and 20.20% of fungal operational taxonomic units (OTUs) in the rhizoplane derived from the endosphere and 20.64% of bacterial and 20.60% of fungal OTUs from the soil. This suggests that the rhizoplane microbial community was a mixed community of soil and endosphere microbial communities and that microorganisms can disperse bidirectionally across different compartments of the plant root. On the other hand, in the absence of an enrichment or depletion of core bacterial and fungal OTUs in the rhizosphere, little differences in microbial composition as well as a more shared microbial network structure between the soil and the rhizosphere support the theory that the rhizosphere microbial community is a subset of the soil community. A large number of functional genes (such as nitrogen fixation and nitrite reduction) and more enriched core OTUs as well as a less stable but more complex network structure were observed in the rhizoplane of rubber tree roots. This demonstrated that the rhizoplane is the most active root compartment and a hotspot for plant–soil–environment interactions. In addition, bacterial and fungal communities in the rhizoplane were more stochastic compared to the rhizosphere and soil.DiscussionOur study expands our understanding of root-associated microbial community structure and function, which may provide the scientific basis for sustainable agriculture through biological process management. |
| first_indexed | 2024-04-09T20:16:18Z |
| format | Article |
| id | doaj.art-4b7558141bc649c082d2a5e753646c78 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T20:16:18Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-4b7558141bc649c082d2a5e753646c782023-03-31T07:13:44ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11364181136418Diversity and assembly of root-associated microbiomes of rubber treesGuoyu Lan0Guoyu Lan1Yaqing Wei2Yaqing Wei3Yuwu Li4Zhixiang Wu5Zhixiang Wu6Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, ChinaTropical Forestry Ecology Group, Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Danzhou, Hainan, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, ChinaCollege of Ecology and Environment, Hainan University, Haikou, Hainan, ChinaCollege of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong, ChinaRubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, ChinaTropical Forestry Ecology Group, Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station, Danzhou, Hainan, ChinaIntroductionUnderstanding the diversity and assembly of the microbiomes of plant roots is crucial to manipulate them for sustainable ecosystem functioning. However, there are few reports about microbial communities at a continuous fine-scale of roots for rubber trees.MethodsWe investigate the structure, diversity, and assembly of bacterial and fungal communities for the soil (non-rhizosphere), rhizosphere, and rhizoplane as well as root endosphere of rubber trees using the amplicon sequencing of 16S ribosomal ribonucleic acid (rRNA) and Internally Transcribed Spacer (ITS) genes.ResultsWe show that 18.69% of bacterial and 20.20% of fungal operational taxonomic units (OTUs) in the rhizoplane derived from the endosphere and 20.64% of bacterial and 20.60% of fungal OTUs from the soil. This suggests that the rhizoplane microbial community was a mixed community of soil and endosphere microbial communities and that microorganisms can disperse bidirectionally across different compartments of the plant root. On the other hand, in the absence of an enrichment or depletion of core bacterial and fungal OTUs in the rhizosphere, little differences in microbial composition as well as a more shared microbial network structure between the soil and the rhizosphere support the theory that the rhizosphere microbial community is a subset of the soil community. A large number of functional genes (such as nitrogen fixation and nitrite reduction) and more enriched core OTUs as well as a less stable but more complex network structure were observed in the rhizoplane of rubber tree roots. This demonstrated that the rhizoplane is the most active root compartment and a hotspot for plant–soil–environment interactions. In addition, bacterial and fungal communities in the rhizoplane were more stochastic compared to the rhizosphere and soil.DiscussionOur study expands our understanding of root-associated microbial community structure and function, which may provide the scientific basis for sustainable agriculture through biological process management.https://www.frontiersin.org/articles/10.3389/fpls.2023.1136418/fullrubber treebacteriafungirhizoplanediversityassembly |
| spellingShingle | Guoyu Lan Guoyu Lan Yaqing Wei Yaqing Wei Yuwu Li Zhixiang Wu Zhixiang Wu Diversity and assembly of root-associated microbiomes of rubber trees Frontiers in Plant Science rubber tree bacteria fungi rhizoplane diversity assembly |
| title | Diversity and assembly of root-associated microbiomes of rubber trees |
| title_full | Diversity and assembly of root-associated microbiomes of rubber trees |
| title_fullStr | Diversity and assembly of root-associated microbiomes of rubber trees |
| title_full_unstemmed | Diversity and assembly of root-associated microbiomes of rubber trees |
| title_short | Diversity and assembly of root-associated microbiomes of rubber trees |
| title_sort | diversity and assembly of root associated microbiomes of rubber trees |
| topic | rubber tree bacteria fungi rhizoplane diversity assembly |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136418/full |
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