Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s disease
Background: Recent evidence has indicated that beta-secretase 1 (BACE1) is involved in the production of amyloid beta (Aβ) in patients affected with Alzheimer’s disease (AD). Therefore; the purpose of this study was to measure mRNA and plasma levels of BACE1 in AD patients, as an early diagnosis bio...
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| Format: | Article |
| Language: | English |
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Babol University of Medical Sciences
2019-06-01
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| Series: | Caspian Journal of Internal Medicine |
| Subjects: | |
| Online Access: | http://caspjim.com/article-1-1635-en.html |
| _version_ | 1828838992587522048 |
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| author | Alireza Vakilian Javad Masoumi Saeid Mirzaee Hossein Khorramdelazad |
| author_facet | Alireza Vakilian Javad Masoumi Saeid Mirzaee Hossein Khorramdelazad |
| author_sort | Alireza Vakilian |
| collection | DOAJ |
| description | Background: Recent evidence has indicated that beta-secretase 1 (BACE1) is involved in the production of amyloid beta (Aβ) in patients affected with Alzheimer’s disease (AD). Therefore; the purpose of this study was to measure mRNA and plasma levels of BACE1 in AD patients, as an early diagnosis biomarker for such individuals.
Methods: A total number of thirty AD patients and thirty normal subjects as controls were recriuted in the present study. Plasma levels of BACE1 were then examined via enzyme-linked immunosorbent assay (ELISA) and also mRNA expression of BACE1 in total blood was measured using real-time PCR technique.
Results: The findings revealed a significant difference in gene expression of BACE1 in the peripheral blood of AD patients compared with that in controls (p<0.0001). Additionally, elevated plasma levels of BACE1 were found in AD patients compared with those in normal subjects (p<0.01). Statistical analyses also demonstrated no correlation between expression (mRNA and protein) of BACE1 in both AD patients and controls and age or the results of Mini-Mental State Examination (MMSE) scale (p>0.05).
Conclusion: Given the importance of early diagnosis of AD patients, it was suggested that the measurement of plasma levels and also mRNA expression of BACE1 might be a valuable blood-based biomarker used in preference to other invasive diagnostic methods such as cerebrospinal fluid (CSF) analysis. |
| first_indexed | 2024-12-12T19:14:09Z |
| format | Article |
| id | doaj.art-4b77eb7b6a534d4799f99f1738b6dd47 |
| institution | Directory Open Access Journal |
| issn | 2008-6164 2008-6172 |
| language | English |
| last_indexed | 2024-12-12T19:14:09Z |
| publishDate | 2019-06-01 |
| publisher | Babol University of Medical Sciences |
| record_format | Article |
| series | Caspian Journal of Internal Medicine |
| spelling | doaj.art-4b77eb7b6a534d4799f99f1738b6dd472022-12-22T00:14:45ZengBabol University of Medical SciencesCaspian Journal of Internal Medicine2008-61642008-61722019-06-01103276280Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s diseaseAlireza Vakilian0Javad Masoumi1Saeid Mirzaee2Hossein Khorramdelazad3 Non Communicable Diseases Research Center, Rafsanjan University of Medical Science, Rafsanjan, Iran Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Background: Recent evidence has indicated that beta-secretase 1 (BACE1) is involved in the production of amyloid beta (Aβ) in patients affected with Alzheimer’s disease (AD). Therefore; the purpose of this study was to measure mRNA and plasma levels of BACE1 in AD patients, as an early diagnosis biomarker for such individuals. Methods: A total number of thirty AD patients and thirty normal subjects as controls were recriuted in the present study. Plasma levels of BACE1 were then examined via enzyme-linked immunosorbent assay (ELISA) and also mRNA expression of BACE1 in total blood was measured using real-time PCR technique. Results: The findings revealed a significant difference in gene expression of BACE1 in the peripheral blood of AD patients compared with that in controls (p<0.0001). Additionally, elevated plasma levels of BACE1 were found in AD patients compared with those in normal subjects (p<0.01). Statistical analyses also demonstrated no correlation between expression (mRNA and protein) of BACE1 in both AD patients and controls and age or the results of Mini-Mental State Examination (MMSE) scale (p>0.05). Conclusion: Given the importance of early diagnosis of AD patients, it was suggested that the measurement of plasma levels and also mRNA expression of BACE1 might be a valuable blood-based biomarker used in preference to other invasive diagnostic methods such as cerebrospinal fluid (CSF) analysis.http://caspjim.com/article-1-1635-en.htmlBACE1Alzheimer’s diseasebiomarker |
| spellingShingle | Alireza Vakilian Javad Masoumi Saeid Mirzaee Hossein Khorramdelazad Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s disease Caspian Journal of Internal Medicine BACE1 Alzheimer’s disease biomarker |
| title | Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s disease |
| title_full | Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s disease |
| title_fullStr | Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s disease |
| title_full_unstemmed | Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s disease |
| title_short | Expression analysis of beta-secretase 1 (BACE1) enzyme in peripheral blood of patients with Alzheimer\'s disease |
| title_sort | expression analysis of beta secretase 1 bace1 enzyme in peripheral blood of patients with alzheimer s disease |
| topic | BACE1 Alzheimer’s disease biomarker |
| url | http://caspjim.com/article-1-1635-en.html |
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