Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in Tomato
Basic leucine zipper (bZIP) transcription factors of the ABA-responsive element binding factor/ABA-responsive element binding proteins (ABF/AREB) subfamily have been implicated in abscisic acid (ABA) and abiotic stress responses in plants. However, the specific function of ABF/AREB transcription fac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/9/1673 |
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| author | Zijian Xu Fan Wang Yongbo Ma Haoran Dang Xiaohui Hu |
| author_facet | Zijian Xu Fan Wang Yongbo Ma Haoran Dang Xiaohui Hu |
| author_sort | Zijian Xu |
| collection | DOAJ |
| description | Basic leucine zipper (bZIP) transcription factors of the ABA-responsive element binding factor/ABA-responsive element binding proteins (ABF/AREB) subfamily have been implicated in abscisic acid (ABA) and abiotic stress responses in plants. However, the specific function of ABF/AREB transcription factors under saline–alkaline stress is unclear. Here, we identified four ABF/AREB transcription factors in tomato and found that SlAREB1 strongly responded to both ABA and saline–alkaline stress. To further explore the function of SlAREB1 under saline–alkaline stress, SlAREB1-overexpressing lines were constructed. Compared with wild-type plants, SlAREB1-overexpressing transgenic tomato plants showed reduced malondialdehyde content, increased the relative water content, and alleviated the degradation of chlorophyll under saline–alkaline stress. Importantly, SlAREB1 directly physically interacted with SlMn-SOD, which improved the activity of antioxidant enzymes and increased the scavenging of excess reactive oxygen species. Overall, the overexpression of SlAREB1 increased the antioxidant capacity of the transgenic tomato under saline–alkaline stress. |
| first_indexed | 2024-03-10T00:53:39Z |
| format | Article |
| id | doaj.art-4b786b16fd2d427e971f68ee0987cde5 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:53:39Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4b786b16fd2d427e971f68ee0987cde52023-11-23T14:47:05ZengMDPI AGAntioxidants2076-39212022-08-01119167310.3390/antiox11091673Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in TomatoZijian Xu0Fan Wang1Yongbo Ma2Haoran Dang3Xiaohui Hu4College of Horticulture, Northwest AF University, Xianyang 712100, ChinaCollege of Horticulture, Northwest AF University, Xianyang 712100, ChinaCollege of Horticulture, Northwest AF University, Xianyang 712100, ChinaCollege of Horticulture, Northwest AF University, Xianyang 712100, ChinaCollege of Horticulture, Northwest AF University, Xianyang 712100, ChinaBasic leucine zipper (bZIP) transcription factors of the ABA-responsive element binding factor/ABA-responsive element binding proteins (ABF/AREB) subfamily have been implicated in abscisic acid (ABA) and abiotic stress responses in plants. However, the specific function of ABF/AREB transcription factors under saline–alkaline stress is unclear. Here, we identified four ABF/AREB transcription factors in tomato and found that SlAREB1 strongly responded to both ABA and saline–alkaline stress. To further explore the function of SlAREB1 under saline–alkaline stress, SlAREB1-overexpressing lines were constructed. Compared with wild-type plants, SlAREB1-overexpressing transgenic tomato plants showed reduced malondialdehyde content, increased the relative water content, and alleviated the degradation of chlorophyll under saline–alkaline stress. Importantly, SlAREB1 directly physically interacted with SlMn-SOD, which improved the activity of antioxidant enzymes and increased the scavenging of excess reactive oxygen species. Overall, the overexpression of SlAREB1 increased the antioxidant capacity of the transgenic tomato under saline–alkaline stress.https://www.mdpi.com/2076-3921/11/9/1673<i>Solanum lycopersicum</i>transcription factor SlAREBsaline–alkaline stressantioxidant enzymes |
| spellingShingle | Zijian Xu Fan Wang Yongbo Ma Haoran Dang Xiaohui Hu Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in Tomato Antioxidants <i>Solanum lycopersicum</i> transcription factor SlAREB saline–alkaline stress antioxidant enzymes |
| title | Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in Tomato |
| title_full | Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in Tomato |
| title_fullStr | Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in Tomato |
| title_full_unstemmed | Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in Tomato |
| title_short | Transcription Factor SlAREB1 Is Involved in the Antioxidant Regulation under Saline–Alkaline Stress in Tomato |
| title_sort | transcription factor slareb1 is involved in the antioxidant regulation under saline alkaline stress in tomato |
| topic | <i>Solanum lycopersicum</i> transcription factor SlAREB saline–alkaline stress antioxidant enzymes |
| url | https://www.mdpi.com/2076-3921/11/9/1673 |
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