Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress
Drought is one of the limiting factors in crop cultivation, such as in oil palm (Elaeis guineensis Jacq.). The transgenic approaches are expected to increase plant tolerance to drought stress and minimize low productivity when drought occurs. Proline is an osmoprotectant compound in plants whi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
indonesian research institute for biotechnology and bioindustry
2020-10-01
|
| Series: | Menara Perkebunan |
| Subjects: | |
| Online Access: | http://mp.iribb.org/index.php/mpjurnal/article/view/386 |
| _version_ | 1819173666026422272 |
|---|---|
| author | Turhadi Hayati Minarsih Imron Riyadi Priyono Asmini Budiani |
| author_facet | Turhadi Hayati Minarsih Imron Riyadi Priyono Asmini Budiani |
| author_sort | Turhadi |
| collection | DOAJ |
| description | Drought is one of the limiting factors in crop
cultivation, such as in oil palm (Elaeis guineensis
Jacq.). The transgenic approaches are expected to
increase plant tolerance to drought stress and
minimize low productivity when drought occurs.
Proline is an osmoprotectant compound in plants
which its biosynthesis involved the P5CS gene.
The objective of this study was to evaluate the
tolerance level of P5CS-transgenic oil palm to
drought stress induced by polyethylene glycol
6000 (PEG-6000). In this present study, the
transgenic and non-transgenic oil palms were
treated by 0, 2, and 4% PEG-6000 under in vitro
conditions. The experiment was arranged as a
factorial completely randomized design with three
replications. The drought level score, total
chlorophyll content, carotenoids, and proline
content, as well as P5CS gene expression in leaf
tissues were observed at 7 and 14 days after stress
treatments. The result showed that transgenic
plantlets had a lower drought level score than those
of non-transgenic lines. A concentration of 4%
PEG-6000 treatment reduced the total chlorophyll
and carotenoids contents than that of 2%
concentration in non-transgenic plantlets at 7 and
14 day after treatments (DAT). In addition, proline
content and P5CS gene expression level in
transgenic had been significantly increased during
stress treatment. Based on these results, it can be
concluded that the P5CS transgene increased the
drought stress tolerance of oil palm. |
| first_indexed | 2024-12-22T20:26:42Z |
| format | Article |
| id | doaj.art-4b7baa6264fa49ba831852ff60b44e87 |
| institution | Directory Open Access Journal |
| issn | 0125-9318 1858-3768 |
| language | English |
| last_indexed | 2024-12-22T20:26:42Z |
| publishDate | 2020-10-01 |
| publisher | indonesian research institute for biotechnology and bioindustry |
| record_format | Article |
| series | Menara Perkebunan |
| spelling | doaj.art-4b7baa6264fa49ba831852ff60b44e872022-12-21T18:13:43Zengindonesian research institute for biotechnology and bioindustryMenara Perkebunan0125-93181858-37682020-10-01882697810.22302/iribb.jur.mp.v88i2.386Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stressTurhadi0Hayati Minarsih1Imron Riyadi2Priyono3Asmini Budiani4Indonesian Research Institute for Biotechnology and Bioindustry Indonesian Research Institute for Biotechnology and BioindustryIndonesian Research Institute for Biotechnology and Bioindustry Indonesian Research Institute for Biotechnology and BioindustryIndonesian Research Institute for Biotechnology and BioindustryDrought is one of the limiting factors in crop cultivation, such as in oil palm (Elaeis guineensis Jacq.). The transgenic approaches are expected to increase plant tolerance to drought stress and minimize low productivity when drought occurs. Proline is an osmoprotectant compound in plants which its biosynthesis involved the P5CS gene. The objective of this study was to evaluate the tolerance level of P5CS-transgenic oil palm to drought stress induced by polyethylene glycol 6000 (PEG-6000). In this present study, the transgenic and non-transgenic oil palms were treated by 0, 2, and 4% PEG-6000 under in vitro conditions. The experiment was arranged as a factorial completely randomized design with three replications. The drought level score, total chlorophyll content, carotenoids, and proline content, as well as P5CS gene expression in leaf tissues were observed at 7 and 14 days after stress treatments. The result showed that transgenic plantlets had a lower drought level score than those of non-transgenic lines. A concentration of 4% PEG-6000 treatment reduced the total chlorophyll and carotenoids contents than that of 2% concentration in non-transgenic plantlets at 7 and 14 day after treatments (DAT). In addition, proline content and P5CS gene expression level in transgenic had been significantly increased during stress treatment. Based on these results, it can be concluded that the P5CS transgene increased the drought stress tolerance of oil palm.http://mp.iribb.org/index.php/mpjurnal/article/view/386carotenoidschlorophylldroughtdrought toleranceproline |
| spellingShingle | Turhadi Hayati Minarsih Imron Riyadi Priyono Asmini Budiani Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress Menara Perkebunan carotenoids chlorophyll drought drought tolerance proline |
| title | Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress |
| title_full | Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress |
| title_fullStr | Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress |
| title_full_unstemmed | Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress |
| title_short | Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress |
| title_sort | physiological responses and p5cs gene expression of transgenic oil palm plantlet induced by drought stress |
| topic | carotenoids chlorophyll drought drought tolerance proline |
| url | http://mp.iribb.org/index.php/mpjurnal/article/view/386 |
| work_keys_str_mv | AT turhadi physiologicalresponsesandp5csgeneexpressionoftransgenicoilpalmplantletinducedbydroughtstress AT hayatiminarsih physiologicalresponsesandp5csgeneexpressionoftransgenicoilpalmplantletinducedbydroughtstress AT imronriyadi physiologicalresponsesandp5csgeneexpressionoftransgenicoilpalmplantletinducedbydroughtstress AT priyono physiologicalresponsesandp5csgeneexpressionoftransgenicoilpalmplantletinducedbydroughtstress AT asminibudiani physiologicalresponsesandp5csgeneexpressionoftransgenicoilpalmplantletinducedbydroughtstress |