Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum)
Premise Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. Methods and Results Forty‐eight primer pairs were designe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-12-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.11306 |
| _version_ | 1818324570390659072 |
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| author | Matthew L. Sheik Kitty L. LaBounty Erika Mitchell Emily L. Gillespie |
| author_facet | Matthew L. Sheik Kitty L. LaBounty Erika Mitchell Emily L. Gillespie |
| author_sort | Matthew L. Sheik |
| collection | DOAJ |
| description | Premise Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. Methods and Results Forty‐eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross‐amplification in other Rhododendron subsect. Ledum species shows broad application of the developed markers within this small, well‐supported clade. Conclusions These microsatellite markers exhibit significant variability and will be useful in population genetics within R. groenlandicum and for investigation of species boundaries across Rhododendron subsect. Ledum. |
| first_indexed | 2024-12-13T11:30:41Z |
| format | Article |
| id | doaj.art-4b7bed60b1674e0c9f4b8efd4877fbb0 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-13T11:30:41Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-4b7bed60b1674e0c9f4b8efd4877fbb02022-12-21T23:47:57ZengWileyApplications in Plant Sciences2168-04502019-12-01712n/an/a10.1002/aps3.11306Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum)Matthew L. Sheik0Kitty L. LaBounty1Erika Mitchell2Emily L. Gillespie3Department of Biology West Virginia University 53 Campus Drive Morgantown West Virginia 26505 USADepartment of Natural Sciences University of Alaska Southeast 1332 Seward Avenue Sitka Alaska 99835 USABetter Life Laboratories 293 George Road East Calais Vermont 05650 USADepartment of Biological Sciences Butler University 4600 Sunset Avenue Indianapolis Indiana 46208 USAPremise Microsatellite markers were developed for Labrador tea (Rhododendron groenlandicum, Ericaceae) to facilitate downstream genetic investigation of this species and the extremely closely related, circumboreal Rhododendron subsect. Ledum. Methods and Results Forty‐eight primer pairs were designed using Illumina data and screened for excellent amplification. Sixteen successful pairs were developed as microsatellite markers using fluorescently labeled amplification to generate chromatogram data. These data were evaluated for intrapopulation and interpopulation variability in three populations from Alaska and Maine, USA, and the Northwest Territories, Canada. Fourteen polymorphic markers genotyped reliably, each with one to eight alleles. Cluster analysis indicates that across the range, populations can be easily discriminated. Cross‐amplification in other Rhododendron subsect. Ledum species shows broad application of the developed markers within this small, well‐supported clade. Conclusions These microsatellite markers exhibit significant variability and will be useful in population genetics within R. groenlandicum and for investigation of species boundaries across Rhododendron subsect. Ledum.https://doi.org/10.1002/aps3.11306EricaceaeLabrador teaRhododendron groenlandicumRhododendron subsect. Ledumspecies boundaries |
| spellingShingle | Matthew L. Sheik Kitty L. LaBounty Erika Mitchell Emily L. Gillespie Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) Applications in Plant Sciences Ericaceae Labrador tea Rhododendron groenlandicum Rhododendron subsect. Ledum species boundaries |
| title | Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
| title_full | Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
| title_fullStr | Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
| title_full_unstemmed | Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
| title_short | Fourteen polymorphic microsatellite markers for the widespread Labrador tea (Rhododendron groenlandicum) |
| title_sort | fourteen polymorphic microsatellite markers for the widespread labrador tea rhododendron groenlandicum |
| topic | Ericaceae Labrador tea Rhododendron groenlandicum Rhododendron subsect. Ledum species boundaries |
| url | https://doi.org/10.1002/aps3.11306 |
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