Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes.
Pasteurella multocida is the primary causative agent of a range of economically important diseases in animals, including haemorrhagic septicaemia (HS), a rapidly fatal disease of ungulates. There is limited information available on the diversity of P. multocida strains that cause HS. Therefore, we d...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4495038?pdf=render |
| _version_ | 1818202658655174656 |
|---|---|
| author | Ahmed M Moustafa Torsten Seemann Simon Gladman Ben Adler Marina Harper John D Boyce Mark D Bennett |
| author_facet | Ahmed M Moustafa Torsten Seemann Simon Gladman Ben Adler Marina Harper John D Boyce Mark D Bennett |
| author_sort | Ahmed M Moustafa |
| collection | DOAJ |
| description | Pasteurella multocida is the primary causative agent of a range of economically important diseases in animals, including haemorrhagic septicaemia (HS), a rapidly fatal disease of ungulates. There is limited information available on the diversity of P. multocida strains that cause HS. Therefore, we determined draft genome sequences of ten disease-causing isolates and two vaccine strains and compared these genomes using a range of bioinformatic analyses. The draft genomes of the 12 HS strains were between 2,298,035 and 2,410,300 bp in length. Comparison of these genomes with the North American HS strain, M1404, and other available P. multocida genomes (Pm70, 3480, 36950 and HN06) identified a core set of 1,824 genes. A set of 96 genes was present in all HS isolates and vaccine strains examined in this study, but absent from Pm70, 3480, 36950 and HN06. Moreover, 59 genes were shared only by the Asian B:2 strains. In two Pakistani isolates, genes with high similarity to genes in the integrative and conjugative element, ICEPmu1 from strain 36950 were identified along with a range of other antimicrobial resistance genes. Phylogenetic analysis indicated that the HS strains formed clades based on their country of isolation. Future analysis of the 96 genes unique to the HS isolates will aid the identification of HS-specific virulence attributes and facilitate the development of disease-specific diagnostic tests. |
| first_indexed | 2024-12-12T03:12:57Z |
| format | Article |
| id | doaj.art-4b7d59b4910d4cf989a539b8279ee3bc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T03:12:57Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4b7d59b4910d4cf989a539b8279ee3bc2022-12-22T00:40:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013029610.1371/journal.pone.0130296Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes.Ahmed M MoustafaTorsten SeemannSimon GladmanBen AdlerMarina HarperJohn D BoyceMark D BennettPasteurella multocida is the primary causative agent of a range of economically important diseases in animals, including haemorrhagic septicaemia (HS), a rapidly fatal disease of ungulates. There is limited information available on the diversity of P. multocida strains that cause HS. Therefore, we determined draft genome sequences of ten disease-causing isolates and two vaccine strains and compared these genomes using a range of bioinformatic analyses. The draft genomes of the 12 HS strains were between 2,298,035 and 2,410,300 bp in length. Comparison of these genomes with the North American HS strain, M1404, and other available P. multocida genomes (Pm70, 3480, 36950 and HN06) identified a core set of 1,824 genes. A set of 96 genes was present in all HS isolates and vaccine strains examined in this study, but absent from Pm70, 3480, 36950 and HN06. Moreover, 59 genes were shared only by the Asian B:2 strains. In two Pakistani isolates, genes with high similarity to genes in the integrative and conjugative element, ICEPmu1 from strain 36950 were identified along with a range of other antimicrobial resistance genes. Phylogenetic analysis indicated that the HS strains formed clades based on their country of isolation. Future analysis of the 96 genes unique to the HS isolates will aid the identification of HS-specific virulence attributes and facilitate the development of disease-specific diagnostic tests.http://europepmc.org/articles/PMC4495038?pdf=render |
| spellingShingle | Ahmed M Moustafa Torsten Seemann Simon Gladman Ben Adler Marina Harper John D Boyce Mark D Bennett Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes. PLoS ONE |
| title | Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes. |
| title_full | Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes. |
| title_fullStr | Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes. |
| title_full_unstemmed | Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes. |
| title_short | Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes. |
| title_sort | comparative genomic analysis of asian haemorrhagic septicaemia associated strains of pasteurella multocida identifies more than 90 haemorrhagic septicaemia specific genes |
| url | http://europepmc.org/articles/PMC4495038?pdf=render |
| work_keys_str_mv | AT ahmedmmoustafa comparativegenomicanalysisofasianhaemorrhagicsepticaemiaassociatedstrainsofpasteurellamultocidaidentifiesmorethan90haemorrhagicsepticaemiaspecificgenes AT torstenseemann comparativegenomicanalysisofasianhaemorrhagicsepticaemiaassociatedstrainsofpasteurellamultocidaidentifiesmorethan90haemorrhagicsepticaemiaspecificgenes AT simongladman comparativegenomicanalysisofasianhaemorrhagicsepticaemiaassociatedstrainsofpasteurellamultocidaidentifiesmorethan90haemorrhagicsepticaemiaspecificgenes AT benadler comparativegenomicanalysisofasianhaemorrhagicsepticaemiaassociatedstrainsofpasteurellamultocidaidentifiesmorethan90haemorrhagicsepticaemiaspecificgenes AT marinaharper comparativegenomicanalysisofasianhaemorrhagicsepticaemiaassociatedstrainsofpasteurellamultocidaidentifiesmorethan90haemorrhagicsepticaemiaspecificgenes AT johndboyce comparativegenomicanalysisofasianhaemorrhagicsepticaemiaassociatedstrainsofpasteurellamultocidaidentifiesmorethan90haemorrhagicsepticaemiaspecificgenes AT markdbennett comparativegenomicanalysisofasianhaemorrhagicsepticaemiaassociatedstrainsofpasteurellamultocidaidentifiesmorethan90haemorrhagicsepticaemiaspecificgenes |