Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens
A comparison of the efficacy of apathogenic genotype I (V4) and lentogenic genotype II (LaSota) strains of live Newcastle disease virus (NDV) vaccines was performed following vaccination with pathogen-associated molecular pattern (PAMP) H9N2 avian influenza vaccine and challenge with velogenic NDV g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/11/1638 |
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| author | Ahmed Elbestawy Hany Ellakany Mahmoud Sedeik Ahmed Gado Mervat Abdel-Latif Ahmed Noreldin Ahmed Orabi Ismail Radwan Wafaa Abd El-Ghany |
| author_facet | Ahmed Elbestawy Hany Ellakany Mahmoud Sedeik Ahmed Gado Mervat Abdel-Latif Ahmed Noreldin Ahmed Orabi Ismail Radwan Wafaa Abd El-Ghany |
| author_sort | Ahmed Elbestawy |
| collection | DOAJ |
| description | A comparison of the efficacy of apathogenic genotype I (V4) and lentogenic genotype II (LaSota) strains of live Newcastle disease virus (NDV) vaccines was performed following vaccination with pathogen-associated molecular pattern (PAMP) H9N2 avian influenza vaccine and challenge with velogenic NDV genotype VII.1.1 (vNDV-VII.1.1). Eight groups (Gs) of day-old chicks were used (<i>n</i> = 25). Groups 1–4 received a single dose of PAMP-H9N2 subcutaneously, while Gs (1, 5) and (2, 6) received eye drops of V4 and LaSota, respectively, as two doses. All Gs, except for 4 and 8, were intramuscularly challenged with vNDV-VII.1.1 at 28 days of age. No signs were detected in Gs 1, 5, 4, and 8. The mortality rates were 0% in Gs 1, 4, 5, and 8; 40% in G2; 46.66% in G6; and 100% in Gs 3 and 7. Lesions were recorded as minimal in Gs 1 and 5, but mild to moderate in Gs 2 and 6. The lowest significant viral shedding was detected in Gs 1, 2, and 5. In conclusion, two successive vaccinations of broilers with a live V4 NDV vaccine provided higher protection against vNDV-VII.1.1 challenge than LaSota. PAMP-H9N2 with live NDV vaccines induced more protection than the live vaccine alone. |
| first_indexed | 2024-03-09T16:23:32Z |
| format | Article |
| id | doaj.art-4b7d5d5d82494fc19765dbd1a0850252 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T16:23:32Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-4b7d5d5d82494fc19765dbd1a08502522023-11-24T15:09:54ZengMDPI AGVaccines2076-393X2023-10-011111163810.3390/vaccines11111638Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler ChickensAhmed Elbestawy0Hany Ellakany1Mahmoud Sedeik2Ahmed Gado3Mervat Abdel-Latif4Ahmed Noreldin5Ahmed Orabi6Ismail Radwan7Wafaa Abd El-Ghany8Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, EgyptDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, EgyptDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, EgyptDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, EgyptNutrition and Veterinary Clinical Nutrition Department, Faculty of Veterinary Medicine, Damanhour University, El-Beheira 22511, EgyptDepartment of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, El-Beheira 22511, EgyptDepartment of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptDepartment of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, EgyptDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptA comparison of the efficacy of apathogenic genotype I (V4) and lentogenic genotype II (LaSota) strains of live Newcastle disease virus (NDV) vaccines was performed following vaccination with pathogen-associated molecular pattern (PAMP) H9N2 avian influenza vaccine and challenge with velogenic NDV genotype VII.1.1 (vNDV-VII.1.1). Eight groups (Gs) of day-old chicks were used (<i>n</i> = 25). Groups 1–4 received a single dose of PAMP-H9N2 subcutaneously, while Gs (1, 5) and (2, 6) received eye drops of V4 and LaSota, respectively, as two doses. All Gs, except for 4 and 8, were intramuscularly challenged with vNDV-VII.1.1 at 28 days of age. No signs were detected in Gs 1, 5, 4, and 8. The mortality rates were 0% in Gs 1, 4, 5, and 8; 40% in G2; 46.66% in G6; and 100% in Gs 3 and 7. Lesions were recorded as minimal in Gs 1 and 5, but mild to moderate in Gs 2 and 6. The lowest significant viral shedding was detected in Gs 1, 2, and 5. In conclusion, two successive vaccinations of broilers with a live V4 NDV vaccine provided higher protection against vNDV-VII.1.1 challenge than LaSota. PAMP-H9N2 with live NDV vaccines induced more protection than the live vaccine alone.https://www.mdpi.com/2076-393X/11/11/1638vNDV-VII.1.1 challengebroiler chickenslive ND vaccinesV4LaSotaPAMP-H9N2 |
| spellingShingle | Ahmed Elbestawy Hany Ellakany Mahmoud Sedeik Ahmed Gado Mervat Abdel-Latif Ahmed Noreldin Ahmed Orabi Ismail Radwan Wafaa Abd El-Ghany Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens Vaccines vNDV-VII.1.1 challenge broiler chickens live ND vaccines V4 LaSota PAMP-H9N2 |
| title | Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens |
| title_full | Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens |
| title_fullStr | Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens |
| title_full_unstemmed | Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens |
| title_short | Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens |
| title_sort | superior efficacy of apathogenic genotype i v4 over lentogenic genotype ii lasota live vaccines against newcastle disease virus genotype vii 1 1 in pathogen associated molecular pattern h9n2 vaccinated broiler chickens |
| topic | vNDV-VII.1.1 challenge broiler chickens live ND vaccines V4 LaSota PAMP-H9N2 |
| url | https://www.mdpi.com/2076-393X/11/11/1638 |
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