Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed Cultivars
Rapeseed is an economically important oilseed crop throughout the world. We examined the content and composition of glucosinolates (GSLs) and phenolics in the sprouts of seven Korean cultivars. A total of eight GSLs that include four aliphatic GSLs (AGSLs) (progoitrin, gluconapin, gluconapoleiferin,...
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MDPI AG
2021-09-01
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| Series: | Plants |
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| author | Joonyup Kim Soo In Sohn Ramaraj Sathasivam Allah Jurio Khaskheli Min Cheol Kim Nam Su Kim Sang Un Park |
| author_facet | Joonyup Kim Soo In Sohn Ramaraj Sathasivam Allah Jurio Khaskheli Min Cheol Kim Nam Su Kim Sang Un Park |
| author_sort | Joonyup Kim |
| collection | DOAJ |
| description | Rapeseed is an economically important oilseed crop throughout the world. We examined the content and composition of glucosinolates (GSLs) and phenolics in the sprouts of seven Korean cultivars. A total of eight GSLs that include four aliphatic GSLs (AGSLs) (progoitrin, gluconapin, gluconapoleiferin, and glucobrassicanapin) and four indole GSLs (IGSLs) (4-methoxyglucobrassicin, 4-hydroxyglucobrassicin, neoglucobrassicin, and glucobrassicin) were identified in these cultivars. Of the total GSLs, the highest level was detected for progoitrin, while the lowest level was identified for glucobrassicanapin in all the cultivars. Phenolics that include chlorogenic acid, catechin hydrate, 4-hydroxybenzoic acid, gallic acid, ferulic acid, <i>p</i>-coumaric acid, epicatechin, caffeic acid, rutin, quercetin, <i>trans</i>-cinnamic acid, benzoic acid, and kaempferol were present in all the cultivars. Of these, rutin was identified with the highest level while <i>trans</i>-cinnamic acid was identified with the lowest level in all the cultivars. Cluster analysis revealed the unique metabolic signature of eight GSLs and thirteen phenolics for the seven cultivars of rapeseed, which implies that genomic commonality and variability resulted from the previous breeding program. Further, gene expression and <i>cis</i>-regulatory elements suggest that the biosynthesis of GSLs and phenolics of these cultivars appears to be regulated through transcription factors associated with stress responses, phytohormones, and cellular growth. |
| first_indexed | 2024-03-10T06:15:11Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T06:15:11Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4b7ec17e455840f696e1552d54dd64ca2023-11-22T19:42:37ZengMDPI AGPlants2223-77472021-09-011010202710.3390/plants10102027Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed CultivarsJoonyup Kim0Soo In Sohn1Ramaraj Sathasivam2Allah Jurio Khaskheli3Min Cheol Kim4Nam Su Kim5Sang Un Park6Department of Horticultural Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaBiosafety Division, Department of Agricultural Biotechnology, Jeonju 54874, KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Horticultural Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaKorea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongju 28116, KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaRapeseed is an economically important oilseed crop throughout the world. We examined the content and composition of glucosinolates (GSLs) and phenolics in the sprouts of seven Korean cultivars. A total of eight GSLs that include four aliphatic GSLs (AGSLs) (progoitrin, gluconapin, gluconapoleiferin, and glucobrassicanapin) and four indole GSLs (IGSLs) (4-methoxyglucobrassicin, 4-hydroxyglucobrassicin, neoglucobrassicin, and glucobrassicin) were identified in these cultivars. Of the total GSLs, the highest level was detected for progoitrin, while the lowest level was identified for glucobrassicanapin in all the cultivars. Phenolics that include chlorogenic acid, catechin hydrate, 4-hydroxybenzoic acid, gallic acid, ferulic acid, <i>p</i>-coumaric acid, epicatechin, caffeic acid, rutin, quercetin, <i>trans</i>-cinnamic acid, benzoic acid, and kaempferol were present in all the cultivars. Of these, rutin was identified with the highest level while <i>trans</i>-cinnamic acid was identified with the lowest level in all the cultivars. Cluster analysis revealed the unique metabolic signature of eight GSLs and thirteen phenolics for the seven cultivars of rapeseed, which implies that genomic commonality and variability resulted from the previous breeding program. Further, gene expression and <i>cis</i>-regulatory elements suggest that the biosynthesis of GSLs and phenolics of these cultivars appears to be regulated through transcription factors associated with stress responses, phytohormones, and cellular growth.https://www.mdpi.com/2223-7747/10/10/2027<i>Brassica napus</i>rapeseedglucosinolatesphenolicsbiosynthesis pathway<i>cis</i>-regulatory elements |
| spellingShingle | Joonyup Kim Soo In Sohn Ramaraj Sathasivam Allah Jurio Khaskheli Min Cheol Kim Nam Su Kim Sang Un Park Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed Cultivars Plants <i>Brassica napus</i> rapeseed glucosinolates phenolics biosynthesis pathway <i>cis</i>-regulatory elements |
| title | Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed Cultivars |
| title_full | Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed Cultivars |
| title_fullStr | Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed Cultivars |
| title_full_unstemmed | Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed Cultivars |
| title_short | Targeted Metabolic and In-Silico Analyses Highlight Distinct Glucosinolates and Phenolics Signatures in Korean Rapeseed Cultivars |
| title_sort | targeted metabolic and in silico analyses highlight distinct glucosinolates and phenolics signatures in korean rapeseed cultivars |
| topic | <i>Brassica napus</i> rapeseed glucosinolates phenolics biosynthesis pathway <i>cis</i>-regulatory elements |
| url | https://www.mdpi.com/2223-7747/10/10/2027 |
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