Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application
Abstract Background Blood transfusions represent common medical procedures, which provide essential supportive therapy. However, these procedures are notoriously expensive for healthcare services and not without risk. The potential threat of transfusion-related complications, such as the development...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-05-01
|
| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-023-03367-8 |
| _version_ | 1797818137092030464 |
|---|---|
| author | Christian Felice Cervellera Chiara Mazziotta Giulia Di Mauro Maria Rosa Iaquinta Elisa Mazzoni Elena Torreggiani Mauro Tognon Fernanda Martini John Charles Rotondo |
| author_facet | Christian Felice Cervellera Chiara Mazziotta Giulia Di Mauro Maria Rosa Iaquinta Elisa Mazzoni Elena Torreggiani Mauro Tognon Fernanda Martini John Charles Rotondo |
| author_sort | Christian Felice Cervellera |
| collection | DOAJ |
| description | Abstract Background Blood transfusions represent common medical procedures, which provide essential supportive therapy. However, these procedures are notoriously expensive for healthcare services and not without risk. The potential threat of transfusion-related complications, such as the development of pathogenic infections and the occurring of alloimmunization events, alongside the donor’s dependence, strongly limits the availability of transfusion units and represents significant concerns in transfusion medicine. Moreover, a further increase in the demand for donated blood and blood transfusion, combined with a reduction in blood donors, is expected as a consequence of the decrease in birth rates and increase in life expectancy in industrialized countries. Main body An emerging and alternative strategy preferred over blood transfusion is the in vitro production of blood cells from immortalized erythroid cells. The high survival capacity alongside the stable and longest proliferation time of immortalized erythroid cells could allow the generation of a large number of cells over time, which are able to differentiate into blood cells. However, a large-scale, cost-effective production of blood cells is not yet a routine clinical procedure, as being dependent on the optimization of culture conditions of immortalized erythroid cells. Conclusion In our review, we provide an overview of the most recent erythroid cell immortalization approaches, while also describing and discussing related advancements of establishing immortalized erythroid cell lines. |
| first_indexed | 2024-03-13T09:03:32Z |
| format | Article |
| id | doaj.art-4b81e481475e4906ad512cbb4f895e47 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-03-13T09:03:32Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-4b81e481475e4906ad512cbb4f895e472023-05-28T11:10:04ZengBMCStem Cell Research & Therapy1757-65122023-05-0114111310.1186/s13287-023-03367-8Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical applicationChristian Felice Cervellera0Chiara Mazziotta1Giulia Di Mauro2Maria Rosa Iaquinta3Elisa Mazzoni4Elena Torreggiani5Mauro Tognon6Fernanda Martini7John Charles Rotondo8Department of Medical Sciences, University of FerraraDepartment of Medical Sciences, University of FerraraDepartment of Medical Sciences, University of FerraraDepartment of Medical Sciences, University of FerraraDepartment of Chemical, Pharmaceutical and Agricultural Sciences—DOCPAS, University of FerraraDepartment of Chemical, Pharmaceutical and Agricultural Sciences—DOCPAS, University of FerraraDepartment of Medical Sciences, University of FerraraDepartment of Medical Sciences, University of FerraraDepartment of Medical Sciences, University of FerraraAbstract Background Blood transfusions represent common medical procedures, which provide essential supportive therapy. However, these procedures are notoriously expensive for healthcare services and not without risk. The potential threat of transfusion-related complications, such as the development of pathogenic infections and the occurring of alloimmunization events, alongside the donor’s dependence, strongly limits the availability of transfusion units and represents significant concerns in transfusion medicine. Moreover, a further increase in the demand for donated blood and blood transfusion, combined with a reduction in blood donors, is expected as a consequence of the decrease in birth rates and increase in life expectancy in industrialized countries. Main body An emerging and alternative strategy preferred over blood transfusion is the in vitro production of blood cells from immortalized erythroid cells. The high survival capacity alongside the stable and longest proliferation time of immortalized erythroid cells could allow the generation of a large number of cells over time, which are able to differentiate into blood cells. However, a large-scale, cost-effective production of blood cells is not yet a routine clinical procedure, as being dependent on the optimization of culture conditions of immortalized erythroid cells. Conclusion In our review, we provide an overview of the most recent erythroid cell immortalization approaches, while also describing and discussing related advancements of establishing immortalized erythroid cell lines.https://doi.org/10.1186/s13287-023-03367-8ErythroblastsImmortalizationHematopoietic stem cellsErythropoiesisCell differentiationGenetic engineering |
| spellingShingle | Christian Felice Cervellera Chiara Mazziotta Giulia Di Mauro Maria Rosa Iaquinta Elisa Mazzoni Elena Torreggiani Mauro Tognon Fernanda Martini John Charles Rotondo Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application Stem Cell Research & Therapy Erythroblasts Immortalization Hematopoietic stem cells Erythropoiesis Cell differentiation Genetic engineering |
| title | Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application |
| title_full | Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application |
| title_fullStr | Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application |
| title_full_unstemmed | Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application |
| title_short | Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application |
| title_sort | immortalized erythroid cells as a novel frontier for in vitro blood production current approaches and potential clinical application |
| topic | Erythroblasts Immortalization Hematopoietic stem cells Erythropoiesis Cell differentiation Genetic engineering |
| url | https://doi.org/10.1186/s13287-023-03367-8 |
| work_keys_str_mv | AT christianfelicecervellera immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT chiaramazziotta immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT giuliadimauro immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT mariarosaiaquinta immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT elisamazzoni immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT elenatorreggiani immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT maurotognon immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT fernandamartini immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication AT johncharlesrotondo immortalizederythroidcellsasanovelfrontierforinvitrobloodproductioncurrentapproachesandpotentialclinicalapplication |