Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice
Abstract Background Psoriasis is a chronic inflammatory skin disease. Tissue stem cells have exhibited a therapeutic effect on psoriatic mice. However, the therapeutic effect of topical administration of the secretome derived from tissue stem cells on psoriasis has not been reported. Methods The sec...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-022-03091-9 |
| _version_ | 1811315279216508928 |
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| author | Mengbo Yang Lanqi Wang Zhimin Chen Weijie Hao Qian You Jianhua Lin Jingzhi Tang Xin Zhao Wei-Qiang Gao Huiming Xu |
| author_facet | Mengbo Yang Lanqi Wang Zhimin Chen Weijie Hao Qian You Jianhua Lin Jingzhi Tang Xin Zhao Wei-Qiang Gao Huiming Xu |
| author_sort | Mengbo Yang |
| collection | DOAJ |
| description | Abstract Background Psoriasis is a chronic inflammatory skin disease. Tissue stem cells have exhibited a therapeutic effect on psoriatic mice. However, the therapeutic effect of topical administration of the secretome derived from tissue stem cells on psoriasis has not been reported. Methods The secretome from human amniotic epithelial cells (AEC-SC) and human umbilical cord mesenchymal stem cells (UMSC-SC) was topically administrated on the back of imiquimod-induced psoriasis-like mice. Subsequently, we observed the skin lesions and skin inflammation of psoriasis-like mice. Next, we further analyzed the paracrine factors in AEC-SC and UMSC-SC by protein chips. Lastly, the effect of the crucial paracrine factor was investigated by imiquimod-induced psoriasis-like mice. Results We found that AEC-SC had a better therapeutic effect on attenuating psoriasis-like skin lesions including skin scales, skin redness and skin thickness than UMSC-SC, and it had a better regulatory effect on keratinocyte hyperproliferation and altered differentiation. Thus, we focused on AEC-SC. Further study showed that AEC-SC reduced the infiltration of neutrophils and interleukin-17-producing T cells. Next, the analysis of AEC-SC with protein chip revealed that the levels of anti-inflammatory factor interleukin-1 receptor antagonist (IL-1ra) were much higher in AEC-SC compared to that in UMSC-SC. More importantly, the beneficial effect of AEC-SC on psoriasis-like skin lesions and skin inflammation of mice were significantly impaired when neutralizing with IL-1ra antibody, while the recombinant human IL-1ra showed a less protective effect than AEC-SC. Conclusions The present study demonstrated that AEC-SC could efficiently ameliorate psoriasis-like skin lesions and skin inflammation and IL-1ra plays an essential role. Therefore, topical administration of AEC-SC may provide a novel strategy for treating psoriasis-like inflammatory skin diseases. |
| first_indexed | 2024-04-13T11:28:31Z |
| format | Article |
| id | doaj.art-4b827f112f4642fe96704c783f3c650b |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-04-13T11:28:31Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-4b827f112f4642fe96704c783f3c650b2022-12-22T02:48:38ZengBMCStem Cell Research & Therapy1757-65122022-08-0113111410.1186/s13287-022-03091-9Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in miceMengbo Yang0Lanqi Wang1Zhimin Chen2Weijie Hao3Qian You4Jianhua Lin5Jingzhi Tang6Xin Zhao7Wei-Qiang Gao8Huiming Xu9State Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Dermatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Dermatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityState Key Laboratory of Oncogenes and Related Genes, Renji-MedX Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong UniversityAbstract Background Psoriasis is a chronic inflammatory skin disease. Tissue stem cells have exhibited a therapeutic effect on psoriatic mice. However, the therapeutic effect of topical administration of the secretome derived from tissue stem cells on psoriasis has not been reported. Methods The secretome from human amniotic epithelial cells (AEC-SC) and human umbilical cord mesenchymal stem cells (UMSC-SC) was topically administrated on the back of imiquimod-induced psoriasis-like mice. Subsequently, we observed the skin lesions and skin inflammation of psoriasis-like mice. Next, we further analyzed the paracrine factors in AEC-SC and UMSC-SC by protein chips. Lastly, the effect of the crucial paracrine factor was investigated by imiquimod-induced psoriasis-like mice. Results We found that AEC-SC had a better therapeutic effect on attenuating psoriasis-like skin lesions including skin scales, skin redness and skin thickness than UMSC-SC, and it had a better regulatory effect on keratinocyte hyperproliferation and altered differentiation. Thus, we focused on AEC-SC. Further study showed that AEC-SC reduced the infiltration of neutrophils and interleukin-17-producing T cells. Next, the analysis of AEC-SC with protein chip revealed that the levels of anti-inflammatory factor interleukin-1 receptor antagonist (IL-1ra) were much higher in AEC-SC compared to that in UMSC-SC. More importantly, the beneficial effect of AEC-SC on psoriasis-like skin lesions and skin inflammation of mice were significantly impaired when neutralizing with IL-1ra antibody, while the recombinant human IL-1ra showed a less protective effect than AEC-SC. Conclusions The present study demonstrated that AEC-SC could efficiently ameliorate psoriasis-like skin lesions and skin inflammation and IL-1ra plays an essential role. Therefore, topical administration of AEC-SC may provide a novel strategy for treating psoriasis-like inflammatory skin diseases.https://doi.org/10.1186/s13287-022-03091-9PsoriasisAEC-SCSkin lesionsSkin inflammationIL-1ra |
| spellingShingle | Mengbo Yang Lanqi Wang Zhimin Chen Weijie Hao Qian You Jianhua Lin Jingzhi Tang Xin Zhao Wei-Qiang Gao Huiming Xu Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice Stem Cell Research & Therapy Psoriasis AEC-SC Skin lesions Skin inflammation IL-1ra |
| title | Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice |
| title_full | Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice |
| title_fullStr | Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice |
| title_full_unstemmed | Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice |
| title_short | Topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis-like skin lesions in mice |
| title_sort | topical administration of the secretome derived from human amniotic epithelial cells ameliorates psoriasis like skin lesions in mice |
| topic | Psoriasis AEC-SC Skin lesions Skin inflammation IL-1ra |
| url | https://doi.org/10.1186/s13287-022-03091-9 |
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