Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
In this study, we aim to discover the importance of long non-coding RNA cyclin-dependent kinase 6 (CDK6)-AS1 in lipopolysaccharide (LPS)-induced HK-2 cells. We treated the HK-2 cells with LPS and knocked down CDK6-AS1 in HK-2 cells and then analyzed the effects of CDK6-AS1 on the viability of cell,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-08-01
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| Series: | Open Medicine |
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| Online Access: | https://doi.org/10.1515/med-2021-0314 |
| _version_ | 1797998364583788544 |
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| author | Wu Ling Zhang Rui Lin Sheng Lin Min Wang Jing |
| author_facet | Wu Ling Zhang Rui Lin Sheng Lin Min Wang Jing |
| author_sort | Wu Ling |
| collection | DOAJ |
| description | In this study, we aim to discover the importance of long non-coding RNA cyclin-dependent kinase 6 (CDK6)-AS1 in lipopolysaccharide (LPS)-induced HK-2 cells. We treated the HK-2 cells with LPS and knocked down CDK6-AS1 in HK-2 cells and then analyzed the effects of CDK6-AS1 on the viability of cell, cell apoptosis, the expression of cytokines via MTT, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and qPCR. The results showed that silencing CDK6-AS1 alleviated LPS-induced inhibition of HK-2 cell proliferation, release of IL-1β, IL-8, IL-6, and TNF-α, cell apoptosis, and decrease in mitochondrial membrane potential. In addition, decreasing the level of CDK6-AS1 inhibited the reduction of Bcl-2 levels, the expression of Bax, cleaved caspade-9, and cleaved caspase-3, induced by LPS. In conclusion, lowering CDK6-AS1 level alleviates LPS-induced inflammatory damage in HK-2 cells. |
| first_indexed | 2024-04-11T10:47:32Z |
| format | Article |
| id | doaj.art-4b84cee53ce249bc8f9ffd1875f9c8ab |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-11T10:47:32Z |
| publishDate | 2021-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-4b84cee53ce249bc8f9ffd1875f9c8ab2022-12-22T04:29:00ZengDe GruyterOpen Medicine2391-54632021-08-011611256126410.1515/med-2021-0314Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cellsWu Ling0Zhang Rui1Lin Sheng2Lin Min3Wang Jing4Department of Pediatrics, Fujian Maternity and Child Health Hospital, Fuzhou 350001, Fujian, ChinaDepartment of Pediatrics, Fujian Maternity and Child Health Hospital, Fuzhou 350001, Fujian, ChinaDepartment of Pediatrics, Fujian Maternity and Child Health Hospital, Fuzhou 350001, Fujian, ChinaPediatric Intensive Care Unit, Fujian Maternity and Child Health Hospital, Fuzhou 350001, Fujian, ChinaDepartment of Nosocomial Infection Management, Fujian Maternity and Child Health Hospital, No. 18 Daoshan Road, Gulou District, Fuzhou 350001, Fujian, ChinaIn this study, we aim to discover the importance of long non-coding RNA cyclin-dependent kinase 6 (CDK6)-AS1 in lipopolysaccharide (LPS)-induced HK-2 cells. We treated the HK-2 cells with LPS and knocked down CDK6-AS1 in HK-2 cells and then analyzed the effects of CDK6-AS1 on the viability of cell, cell apoptosis, the expression of cytokines via MTT, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and qPCR. The results showed that silencing CDK6-AS1 alleviated LPS-induced inhibition of HK-2 cell proliferation, release of IL-1β, IL-8, IL-6, and TNF-α, cell apoptosis, and decrease in mitochondrial membrane potential. In addition, decreasing the level of CDK6-AS1 inhibited the reduction of Bcl-2 levels, the expression of Bax, cleaved caspade-9, and cleaved caspase-3, induced by LPS. In conclusion, lowering CDK6-AS1 level alleviates LPS-induced inflammatory damage in HK-2 cells.https://doi.org/10.1515/med-2021-0314long non-coding rnalpscdk6-as1apoptosis |
| spellingShingle | Wu Ling Zhang Rui Lin Sheng Lin Min Wang Jing Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells Open Medicine long non-coding rna lps cdk6-as1 apoptosis |
| title | Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells |
| title_full | Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells |
| title_fullStr | Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells |
| title_full_unstemmed | Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells |
| title_short | Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells |
| title_sort | silencing cdk6 as1 inhibits lps induced inflammatory damage in hk 2 cells |
| topic | long non-coding rna lps cdk6-as1 apoptosis |
| url | https://doi.org/10.1515/med-2021-0314 |
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