The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapy
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2011-04-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/18015.pdf&manuscript_id=18015 |
| _version_ | 1819180497290395648 |
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| author | Asadi M Farokhi F Ganji Bakhsh M Delirezh N Nejati V Gholami K |
| author_facet | Asadi M Farokhi F Ganji Bakhsh M Delirezh N Nejati V Gholami K |
| author_sort | Asadi M |
| collection | DOAJ |
| description | "n 800x600 Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} Background: Nowadays, dendritic cells (DC) are used for tumor immunotherapy as they can induce immune responses against tumor cells. In this research, we comprehensively studied the maturation stimulus addition, PHA-activated T-cell (PHA-TCM) conditioned medium, autologous monocyte-conditioned medium (MCM) and TNF-α for their ability to promote uniformly mature dendritic cells that elicit T-cell responses."n"nMethods: Plastic adherent monocytes were cultured with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) for five days and two days with monocyte-conditioned medium (MCM), tumor necrotizing factor-α (TNF-α) without TCM (PHA-activated T-cell conditioned medium). Phenotypic and functional analyses were carried out using anti-CD14, anti-CD80, anti-CD86, anti-CD83 monoclonal antibodies. Phagocytic activity, mixed lymphocyte reaction (MLR) and cytokine production were also evaluated."n"nResults: The generated dendritic cells had high expression of surface molecules i.e. CD80, CD83, CD86 and HLA-DR. Moreover, the cells had low phagocytic and high T-lymphocyte stimulating activities. Measurement of the produced cytokines showed the generation of type-1 dendritic cells (DC1) in the study."n"nConclusion: The findings indicated that more efficient maturation of dendritic cells could be achieved by the use of PHA-activated T-lymphocyte conditioned medium in the culture medium. The aforesaid supernatant can be used as a maturation factor for the production of efficient dendritic cells with the ability to be used for tumor immunotherapy. This conditioned medium can provide new strategies and evolve into more advance tools for the generation of dendritic cells in vitro for tumor immunotherapy. |
| first_indexed | 2024-12-22T22:15:17Z |
| format | Article |
| id | doaj.art-4b87f08805f842c790bfc661c949f10a |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-22T22:15:17Z |
| publishDate | 2011-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-4b87f08805f842c790bfc661c949f10a2022-12-21T18:10:47ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222011-04-0169119The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapyAsadi MFarokhi FGanji Bakhsh MDelirezh NNejati VGholami K"n 800x600 Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif";} Background: Nowadays, dendritic cells (DC) are used for tumor immunotherapy as they can induce immune responses against tumor cells. In this research, we comprehensively studied the maturation stimulus addition, PHA-activated T-cell (PHA-TCM) conditioned medium, autologous monocyte-conditioned medium (MCM) and TNF-α for their ability to promote uniformly mature dendritic cells that elicit T-cell responses."n"nMethods: Plastic adherent monocytes were cultured with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) for five days and two days with monocyte-conditioned medium (MCM), tumor necrotizing factor-α (TNF-α) without TCM (PHA-activated T-cell conditioned medium). Phenotypic and functional analyses were carried out using anti-CD14, anti-CD80, anti-CD86, anti-CD83 monoclonal antibodies. Phagocytic activity, mixed lymphocyte reaction (MLR) and cytokine production were also evaluated."n"nResults: The generated dendritic cells had high expression of surface molecules i.e. CD80, CD83, CD86 and HLA-DR. Moreover, the cells had low phagocytic and high T-lymphocyte stimulating activities. Measurement of the produced cytokines showed the generation of type-1 dendritic cells (DC1) in the study."n"nConclusion: The findings indicated that more efficient maturation of dendritic cells could be achieved by the use of PHA-activated T-lymphocyte conditioned medium in the culture medium. The aforesaid supernatant can be used as a maturation factor for the production of efficient dendritic cells with the ability to be used for tumor immunotherapy. This conditioned medium can provide new strategies and evolve into more advance tools for the generation of dendritic cells in vitro for tumor immunotherapy.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/18015.pdf&manuscript_id=18015Conditioned mediadendritic cellsT-lymphocytetumor immunotherapy |
| spellingShingle | Asadi M Farokhi F Ganji Bakhsh M Delirezh N Nejati V Gholami K The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapy Tehran University Medical Journal Conditioned media dendritic cells T-lymphocyte tumor immunotherapy |
| title | The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapy |
| title_full | The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapy |
| title_fullStr | The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapy |
| title_full_unstemmed | The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapy |
| title_short | The effects of T-cell conditioned media on the induction of dendritic cell (DC1) maturation for effective tumor immunotherapy |
| title_sort | effects of t cell conditioned media on the induction of dendritic cell dc1 maturation for effective tumor immunotherapy |
| topic | Conditioned media dendritic cells T-lymphocyte tumor immunotherapy |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/18015.pdf&manuscript_id=18015 |
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