Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film Itself
The lack of suitable functional groups for cell adhesion on the surface of Polycaprolactone (PCL) is one of the main limitations in order to use PCL for biomedical applications. The aim of this research is to modify the PCL film surface using arginine, via an aminolysis reaction. In this regard, aft...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/21/4654 |
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| author | Yuliya Nashchekina Alina Chabina Olga Moskalyuk Irina Voronkina Polina Evstigneeva Gleb Vaganov Alexey Nashchekin Vladimir Yudin Nataliya Mikhailova |
| author_facet | Yuliya Nashchekina Alina Chabina Olga Moskalyuk Irina Voronkina Polina Evstigneeva Gleb Vaganov Alexey Nashchekin Vladimir Yudin Nataliya Mikhailova |
| author_sort | Yuliya Nashchekina |
| collection | DOAJ |
| description | The lack of suitable functional groups for cell adhesion on the surface of Polycaprolactone (PCL) is one of the main limitations in order to use PCL for biomedical applications. The aim of this research is to modify the PCL film surface using arginine, via an aminolysis reaction. In this regard, after PCL films formation by casting method, they were immersed in arginine solutions of various concentration at room temperature or then heated to 40 °C and in the presence of isopropanol or without it. To assess the structure of the modified surface, its wettability, and mechanical properties, methods of measuring the contact angle and the strip tensile test were used, and to compare the degree of attachment and the rate of cell proliferation, the method of fluorescent staining of cultured cells was used. The change in protein synthesis by cells on the modified surface was assessed using Western blotting. The results obtained show that the treatment of PCL films with an aqueous solution of arginine at room temperature for 1 day increases the hydrophilicity of the surface. Wherein surface modification led to a two-fold decrease of mechanical strength and flow stress, but elongation increase by about 30% for PCL films after modification in 0.5 M aqueous arginine solution at room temperature. Moreover, cell attachment and proliferation, as well as collagen synthesis, were significantly enhanced after arginine modification. The proposed simple and effective method for modifying PCL films with arginine significantly expands the possibilities for developing biocompatible scaffolds for tissue engineering. |
| first_indexed | 2024-03-09T18:42:54Z |
| format | Article |
| id | doaj.art-4b8e23c6b46e48159e387b0d5e76ea72 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T18:42:54Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-4b8e23c6b46e48159e387b0d5e76ea722023-11-24T06:29:43ZengMDPI AGPolymers2073-43602022-11-011421465410.3390/polym14214654Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film ItselfYuliya Nashchekina0Alina Chabina1Olga Moskalyuk2Irina Voronkina3Polina Evstigneeva4Gleb Vaganov5Alexey Nashchekin6Vladimir Yudin7Nataliya Mikhailova8Center of Cell Technologies, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky pr. 4, 194064 St. Petersburg, RussiaCenter of Cell Technologies, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky pr. 4, 194064 St. Petersburg, RussiaLaboratory of Polymer and Composite Materials «SmartTextiles», IRC–X-ray Coherent Optics, Immanuel Kant Baltic Federal University, 236029 Kaliningrad, RussiaBiochemistry Department, Institute of Experimental Medicine, Akad. Pavlov Street, 12, 197376 St. Petersburg, RussiaBiochemistry Department, Institute of Experimental Medicine, Akad. Pavlov Street, 12, 197376 St. Petersburg, RussiaInstitute of Macromolecular Compounds of Russian Academy of Sciences, V.O., Bol’shoy pr. 31, 199004 St. Petersburg, RussiaLaboratory «Characterization of Materials and Structures of Solid State Electronics», Ioffe Institute, Polytekhnicheskaya str., 26, 194021 St. Petersburg, RussiaInstitute of Macromolecular Compounds of Russian Academy of Sciences, V.O., Bol’shoy pr. 31, 199004 St. Petersburg, RussiaCenter of Cell Technologies, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky pr. 4, 194064 St. Petersburg, RussiaThe lack of suitable functional groups for cell adhesion on the surface of Polycaprolactone (PCL) is one of the main limitations in order to use PCL for biomedical applications. The aim of this research is to modify the PCL film surface using arginine, via an aminolysis reaction. In this regard, after PCL films formation by casting method, they were immersed in arginine solutions of various concentration at room temperature or then heated to 40 °C and in the presence of isopropanol or without it. To assess the structure of the modified surface, its wettability, and mechanical properties, methods of measuring the contact angle and the strip tensile test were used, and to compare the degree of attachment and the rate of cell proliferation, the method of fluorescent staining of cultured cells was used. The change in protein synthesis by cells on the modified surface was assessed using Western blotting. The results obtained show that the treatment of PCL films with an aqueous solution of arginine at room temperature for 1 day increases the hydrophilicity of the surface. Wherein surface modification led to a two-fold decrease of mechanical strength and flow stress, but elongation increase by about 30% for PCL films after modification in 0.5 M aqueous arginine solution at room temperature. Moreover, cell attachment and proliferation, as well as collagen synthesis, were significantly enhanced after arginine modification. The proposed simple and effective method for modifying PCL films with arginine significantly expands the possibilities for developing biocompatible scaffolds for tissue engineering.https://www.mdpi.com/2073-4360/14/21/4654polycaprolactone filmsargininemechanical propertiesmesenchymal stem cellsextracellular matrix protein |
| spellingShingle | Yuliya Nashchekina Alina Chabina Olga Moskalyuk Irina Voronkina Polina Evstigneeva Gleb Vaganov Alexey Nashchekin Vladimir Yudin Nataliya Mikhailova Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film Itself Polymers polycaprolactone films arginine mechanical properties mesenchymal stem cells extracellular matrix protein |
| title | Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film Itself |
| title_full | Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film Itself |
| title_fullStr | Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film Itself |
| title_full_unstemmed | Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film Itself |
| title_short | Effect of Functionalization of the Polycaprolactone Film Surface on the Mechanical and Biological Properties of the Film Itself |
| title_sort | effect of functionalization of the polycaprolactone film surface on the mechanical and biological properties of the film itself |
| topic | polycaprolactone films arginine mechanical properties mesenchymal stem cells extracellular matrix protein |
| url | https://www.mdpi.com/2073-4360/14/21/4654 |
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