Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying Fungi
The authors have recently investigated the chemical components and bioactivity of fungus comb from <i>Macrotermes gilvus</i> Hagen mounds. The ethyl acetate, methanol, and water extracts of the fungus comb contained active compounds which are preventing the growth of <i>Aspergillus...
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MDPI AG
2023-05-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/6/1115 |
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| author | Dodi Nandika Arinana Arinana Lina Karlinasari Irmanida Batubara Djoko Santoso Lucia Dhiantika Witasari Yanti Rachmayanti Dikhi Firmansyah I Ketut Sudiana Decsa Medika Hertanto Yusuf Sudo Hadi Mohamad Miftah Rahman |
| author_facet | Dodi Nandika Arinana Arinana Lina Karlinasari Irmanida Batubara Djoko Santoso Lucia Dhiantika Witasari Yanti Rachmayanti Dikhi Firmansyah I Ketut Sudiana Decsa Medika Hertanto Yusuf Sudo Hadi Mohamad Miftah Rahman |
| author_sort | Dodi Nandika |
| collection | DOAJ |
| description | The authors have recently investigated the chemical components and bioactivity of fungus comb from <i>Macrotermes gilvus</i> Hagen mounds. The ethyl acetate, methanol, and water extracts of the fungus comb contained active compounds which are preventing the growth of <i>Aspergillus foeti-dus</i>, one of the most economically important wood-staining fungi in Indonesia. In this present study, the bioactivity of the fungus comb extracts was examined against the white-rot fungus <i>Schizophyllum commune</i> Fr. For the purpose of generating a realistic in-service type of environment, the extracts were evaluated according to modified EN-113 after impregnated into wood samples by the vacuum-pressure method, following in-vitro antimicrobial susceptibility test. The results showed that ethyl acetate extract at concentrations ranging from 2 to 6% and methanol extract at a concentration of 6% presented high bioactivity against <i>S. commune</i>. This result was established through optical microscopy images, which demonstrated the absence of fungal mycelia in the vessels of wood samples treated with EtOAc extract at concentrations of 2%, 4%, and 6%, as well as MeOH extract with a concentration of 6%. The toxic values of the ethyl acetate and methanol extracts were determined to be 6.17% and 7.72%, respectively. Based on UPLC-HRMS analysis, azelaic acid, and erucamide were discovered as the dominant components in ethyl acetate extracts, which are anticipated to be the most active compounds. It appears that ethyl acetate extract, as well as methanol extract, can be considered as novel preservative sources for controlling wood-decaying fungi. |
| first_indexed | 2024-03-11T02:27:29Z |
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| id | doaj.art-4b8e8d6ad5444a1e9561faa9deb6f6b6 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T02:27:29Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-4b8e8d6ad5444a1e9561faa9deb6f6b62023-11-18T10:26:35ZengMDPI AGForests1999-49072023-05-01146111510.3390/f14061115Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying FungiDodi Nandika0Arinana Arinana1Lina Karlinasari2Irmanida Batubara3Djoko Santoso4Lucia Dhiantika Witasari5Yanti Rachmayanti6Dikhi Firmansyah7I Ketut Sudiana8Decsa Medika Hertanto9Yusuf Sudo Hadi10Mohamad Miftah Rahman11Department of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, IndonesiaDepartment of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, IndonesiaDepartment of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, and Tropical Biopharmaca Research Center, IPB University, Darmaga Campus, Bogor 16680, West Java, IndonesiaFaculty of Medicine, Campus A Universitas Airlangga, Surabaya 60132, East Java, IndonesiaFaculty of Agricultural Technology, Gadjah Mada University, Yogyakarta 55281, Bulaksumur, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung 40132, West Java, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung 40132, West Java, IndonesiaFaculty of Medicine, Campus A Universitas Airlangga, Surabaya 60132, East Java, IndonesiaFaculty of Medicine, Campus A Universitas Airlangga, Surabaya 60132, East Java, IndonesiaDepartment of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, IndonesiaDepartment of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, IndonesiaThe authors have recently investigated the chemical components and bioactivity of fungus comb from <i>Macrotermes gilvus</i> Hagen mounds. The ethyl acetate, methanol, and water extracts of the fungus comb contained active compounds which are preventing the growth of <i>Aspergillus foeti-dus</i>, one of the most economically important wood-staining fungi in Indonesia. In this present study, the bioactivity of the fungus comb extracts was examined against the white-rot fungus <i>Schizophyllum commune</i> Fr. For the purpose of generating a realistic in-service type of environment, the extracts were evaluated according to modified EN-113 after impregnated into wood samples by the vacuum-pressure method, following in-vitro antimicrobial susceptibility test. The results showed that ethyl acetate extract at concentrations ranging from 2 to 6% and methanol extract at a concentration of 6% presented high bioactivity against <i>S. commune</i>. This result was established through optical microscopy images, which demonstrated the absence of fungal mycelia in the vessels of wood samples treated with EtOAc extract at concentrations of 2%, 4%, and 6%, as well as MeOH extract with a concentration of 6%. The toxic values of the ethyl acetate and methanol extracts were determined to be 6.17% and 7.72%, respectively. Based on UPLC-HRMS analysis, azelaic acid, and erucamide were discovered as the dominant components in ethyl acetate extracts, which are anticipated to be the most active compounds. It appears that ethyl acetate extract, as well as methanol extract, can be considered as novel preservative sources for controlling wood-decaying fungi.https://www.mdpi.com/1999-4907/14/6/1115active extract<i>Macrotermes gilvus</i><i>Schizophyllum commune</i> Fr. LPH 01UPLC-HRMS |
| spellingShingle | Dodi Nandika Arinana Arinana Lina Karlinasari Irmanida Batubara Djoko Santoso Lucia Dhiantika Witasari Yanti Rachmayanti Dikhi Firmansyah I Ketut Sudiana Decsa Medika Hertanto Yusuf Sudo Hadi Mohamad Miftah Rahman Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying Fungi Forests active extract <i>Macrotermes gilvus</i> <i>Schizophyllum commune</i> Fr. LPH 01 UPLC-HRMS |
| title | Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying Fungi |
| title_full | Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying Fungi |
| title_fullStr | Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying Fungi |
| title_full_unstemmed | Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying Fungi |
| title_short | Efficacy of Fungus Comb Extracts Isolated from Indo-Malayan Termite Mounds in Controlling Wood-Decaying Fungi |
| title_sort | efficacy of fungus comb extracts isolated from indo malayan termite mounds in controlling wood decaying fungi |
| topic | active extract <i>Macrotermes gilvus</i> <i>Schizophyllum commune</i> Fr. LPH 01 UPLC-HRMS |
| url | https://www.mdpi.com/1999-4907/14/6/1115 |
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