Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis
The genus Weissella is attracting an increasing amount of attention because of its multiple functions and probiotic potential. In particular, the species Weissella confusa is known to have great potential in industrial applications and exhibits numerous biological functions. However, the knowledge o...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.749218/full |
| _version_ | 1818597755176615936 |
|---|---|
| author | Shenglei Yuan Shenglei Yuan Yundan Wang Yundan Wang Fangqing Zhao Le Kang Le Kang Le Kang Le Kang |
| author_facet | Shenglei Yuan Shenglei Yuan Yundan Wang Yundan Wang Fangqing Zhao Le Kang Le Kang Le Kang Le Kang |
| author_sort | Shenglei Yuan |
| collection | DOAJ |
| description | The genus Weissella is attracting an increasing amount of attention because of its multiple functions and probiotic potential. In particular, the species Weissella confusa is known to have great potential in industrial applications and exhibits numerous biological functions. However, the knowledge on this bacterium in insects is not investigated. Here, we isolated and identified W. confusa as the dominant lactic acid bacteria in the gut of the migratory locust. We named this strain W. confusa LM1, which is the first genome of an insect-derived W. confusa strain with one complete chromosome and one complete plasmid. Among all W. confusa strains, W. confusa LM1 had the largest genome. Its genome was the closest to that of W. confusa 1001271B_151109_G12, a strain from human feces. Our results provided accurate evolutionary relationships of known Weissella species and W. confusa strains. Based on genomic analysis, the pan-genome of W. confusa is in an open state. Most strains of W. confusa had the unique genes, indicating that these strains can adapt to different ecological niches and organisms. However, the variation of strain-specific genes did represent significant correlations with their hosts and ecological niches. These strains were predicted to have low potential to produce secondary metabolites. Furthermore, no antibiotic resistance genes were identified. At the same time, virulence factors associated with toxin production and secretion system were not found, indicating that W. confusa strains were not sufficient to perform virulence. Our study facilitated the discovery of the functions of W. confusa LM1 in locust biology and their potential application to locust management. |
| first_indexed | 2024-12-16T11:52:50Z |
| format | Article |
| id | doaj.art-4b908a985bb84f359230f93676d03114 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T11:52:50Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4b908a985bb84f359230f93676d031142022-12-21T22:32:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-09-011210.3389/fmicb.2021.749218749218Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic AnalysisShenglei Yuan0Shenglei Yuan1Yundan Wang2Yundan Wang3Fangqing Zhao4Le Kang5Le Kang6Le Kang7Le Kang8State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaBeijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, ChinaBeijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Science, Hebei University, Baoding, ChinaThe genus Weissella is attracting an increasing amount of attention because of its multiple functions and probiotic potential. In particular, the species Weissella confusa is known to have great potential in industrial applications and exhibits numerous biological functions. However, the knowledge on this bacterium in insects is not investigated. Here, we isolated and identified W. confusa as the dominant lactic acid bacteria in the gut of the migratory locust. We named this strain W. confusa LM1, which is the first genome of an insect-derived W. confusa strain with one complete chromosome and one complete plasmid. Among all W. confusa strains, W. confusa LM1 had the largest genome. Its genome was the closest to that of W. confusa 1001271B_151109_G12, a strain from human feces. Our results provided accurate evolutionary relationships of known Weissella species and W. confusa strains. Based on genomic analysis, the pan-genome of W. confusa is in an open state. Most strains of W. confusa had the unique genes, indicating that these strains can adapt to different ecological niches and organisms. However, the variation of strain-specific genes did represent significant correlations with their hosts and ecological niches. These strains were predicted to have low potential to produce secondary metabolites. Furthermore, no antibiotic resistance genes were identified. At the same time, virulence factors associated with toxin production and secretion system were not found, indicating that W. confusa strains were not sufficient to perform virulence. Our study facilitated the discovery of the functions of W. confusa LM1 in locust biology and their potential application to locust management.https://www.frontiersin.org/articles/10.3389/fmicb.2021.749218/fullLocusta migratoriagut bacteriaWeissella confusacomparative genomicspan-genome |
| spellingShingle | Shenglei Yuan Shenglei Yuan Yundan Wang Yundan Wang Fangqing Zhao Le Kang Le Kang Le Kang Le Kang Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis Frontiers in Microbiology Locusta migratoria gut bacteria Weissella confusa comparative genomics pan-genome |
| title | Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis |
| title_full | Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis |
| title_fullStr | Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis |
| title_full_unstemmed | Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis |
| title_short | Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis |
| title_sort | complete genome sequence of weissella confusa lm1 and comparative genomic analysis |
| topic | Locusta migratoria gut bacteria Weissella confusa comparative genomics pan-genome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.749218/full |
| work_keys_str_mv | AT shengleiyuan completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT shengleiyuan completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT yundanwang completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT yundanwang completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT fangqingzhao completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT lekang completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT lekang completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT lekang completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis AT lekang completegenomesequenceofweissellaconfusalm1andcomparativegenomicanalysis |