Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro
Noise-induced hearing loss (NIHL) is characterized by cellular damage to the inner ear, which is exacerbated by inflammation. High-mobility group box 1 (HMGB1), a representative damage-associated molecular pattern (DAMP), acts as a mediator of inflammation or an intercellular messenger according to...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.642946/full |
| _version_ | 1818624188820226048 |
|---|---|
| author | Lili Xiao Lili Xiao Lili Xiao Yan Sun Chengqi Liu Chengqi Liu Chengqi Liu Zhong Zheng Zhong Zheng Zhong Zheng Ying Shen Ying Shen Ying Shen Liang Xia Liang Xia Liang Xia Guang Yang Guang Yang Guang Yang Yanmei Feng Yanmei Feng Yanmei Feng |
| author_facet | Lili Xiao Lili Xiao Lili Xiao Yan Sun Chengqi Liu Chengqi Liu Chengqi Liu Zhong Zheng Zhong Zheng Zhong Zheng Ying Shen Ying Shen Ying Shen Liang Xia Liang Xia Liang Xia Guang Yang Guang Yang Guang Yang Yanmei Feng Yanmei Feng Yanmei Feng |
| author_sort | Lili Xiao |
| collection | DOAJ |
| description | Noise-induced hearing loss (NIHL) is characterized by cellular damage to the inner ear, which is exacerbated by inflammation. High-mobility group box 1 (HMGB1), a representative damage-associated molecular pattern (DAMP), acts as a mediator of inflammation or an intercellular messenger according to its cellular localization. Blocking or regulating HMGB1 offers an attractive approach in ameliorating NIHL. However, the precise therapeutic intervention must be based on a deeper understanding of its dynamic molecular distribution and function in cochlear pathogenesis after acoustic trauma. Here, we have presented the spatiotemporal dynamics of the expression of HMGB1, exhibiting distribution variability in specific cochlear regions and cells following noise exposure. After gene manipulation, we further investigated the characteristics of cellular HMGB1 in HEI-OC1 cells. The higher cell viability observed in the HMGB1 knocked-down group after stimulation with H2O2 indicated the possible negative effect of HMGB1 on cellular lifespan. In conclusion, this study demonstrated that HMGB1 is involved in NIHL pathogenesis and its molecular biology has essential and subtle influences, preserving a translational potential for pharmacological intervention. |
| first_indexed | 2024-12-16T18:53:00Z |
| format | Article |
| id | doaj.art-4b92400b3d344f0cbf0094fa84330811 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-16T18:53:00Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-4b92400b3d344f0cbf0094fa843308112022-12-21T22:20:38ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-02-01910.3389/fcell.2021.642946642946Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitroLili Xiao0Lili Xiao1Lili Xiao2Yan Sun3Chengqi Liu4Chengqi Liu5Chengqi Liu6Zhong Zheng7Zhong Zheng8Zhong Zheng9Ying Shen10Ying Shen11Ying Shen12Liang Xia13Liang Xia14Liang Xia15Guang Yang16Guang Yang17Guang Yang18Yanmei Feng19Yanmei Feng20Yanmei Feng21Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaOtolaryngology Institute of Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, ChinaZhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaOtolaryngology Institute of Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaOtolaryngology Institute of Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaOtolaryngology Institute of Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaOtolaryngology Institute of Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaOtolaryngology Institute of Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, ChinaOtolaryngology Institute of Shanghai Jiao Tong University, Shanghai, ChinaShanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, ChinaNoise-induced hearing loss (NIHL) is characterized by cellular damage to the inner ear, which is exacerbated by inflammation. High-mobility group box 1 (HMGB1), a representative damage-associated molecular pattern (DAMP), acts as a mediator of inflammation or an intercellular messenger according to its cellular localization. Blocking or regulating HMGB1 offers an attractive approach in ameliorating NIHL. However, the precise therapeutic intervention must be based on a deeper understanding of its dynamic molecular distribution and function in cochlear pathogenesis after acoustic trauma. Here, we have presented the spatiotemporal dynamics of the expression of HMGB1, exhibiting distribution variability in specific cochlear regions and cells following noise exposure. After gene manipulation, we further investigated the characteristics of cellular HMGB1 in HEI-OC1 cells. The higher cell viability observed in the HMGB1 knocked-down group after stimulation with H2O2 indicated the possible negative effect of HMGB1 on cellular lifespan. In conclusion, this study demonstrated that HMGB1 is involved in NIHL pathogenesis and its molecular biology has essential and subtle influences, preserving a translational potential for pharmacological intervention.https://www.frontiersin.org/articles/10.3389/fcell.2021.642946/fullnoisehearing losscochleahigh-mobility group box 1spatiotemporal distribution |
| spellingShingle | Lili Xiao Lili Xiao Lili Xiao Yan Sun Chengqi Liu Chengqi Liu Chengqi Liu Zhong Zheng Zhong Zheng Zhong Zheng Ying Shen Ying Shen Ying Shen Liang Xia Liang Xia Liang Xia Guang Yang Guang Yang Guang Yang Yanmei Feng Yanmei Feng Yanmei Feng Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro Frontiers in Cell and Developmental Biology noise hearing loss cochlea high-mobility group box 1 spatiotemporal distribution |
| title | Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro |
| title_full | Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro |
| title_fullStr | Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro |
| title_full_unstemmed | Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro |
| title_short | Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro |
| title_sort | molecular behavior of hmgb1 in the cochlea following noise exposure and in vitro |
| topic | noise hearing loss cochlea high-mobility group box 1 spatiotemporal distribution |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.642946/full |
| work_keys_str_mv | AT lilixiao molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT lilixiao molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT lilixiao molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT yansun molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT chengqiliu molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT chengqiliu molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT chengqiliu molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT zhongzheng molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT zhongzheng molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT zhongzheng molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT yingshen molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT yingshen molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT yingshen molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT liangxia molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT liangxia molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT liangxia molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT guangyang molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT guangyang molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT guangyang molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT yanmeifeng molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT yanmeifeng molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro AT yanmeifeng molecularbehaviorofhmgb1inthecochleafollowingnoiseexposureandinvitro |