The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle
<i>Staphylococcus aureus</i> is one of the leading causes of bovine mastitis worldwide and is a common indication for use of antimicrobials on dairy farms. This study aims to investigate the association between on-farm antimicrobial usage and the antimicrobial resistance (AMR) profiles o...
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MDPI AG
2021-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/8/11/287 |
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| author | Sabrina S. Greening Ji Zhang Anne C. Midwinter David A. Wilkinson Scott McDougall M. Carolyn Gates Nigel P. French |
| author_facet | Sabrina S. Greening Ji Zhang Anne C. Midwinter David A. Wilkinson Scott McDougall M. Carolyn Gates Nigel P. French |
| author_sort | Sabrina S. Greening |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> is one of the leading causes of bovine mastitis worldwide and is a common indication for use of antimicrobials on dairy farms. This study aims to investigate the association between on-farm antimicrobial usage and the antimicrobial resistance (AMR) profiles of mastitis-causing <i>S. aureus</i>. Whole-genome sequencing was performed on 57 <i>S. aureus</i> isolates derived from cows with either clinical or subclinical mastitis from 17 dairy herds in New Zealand. The genetic relatedness between isolates was examined using the core single nucleotide polymorphism alignment whilst AMR and virulence genes were identified <i>in-silico</i>. The association between gene presence-absence and sequence type (ST), antimicrobial susceptibility and dry cow therapy treatment was investigated using Scoary. Altogether, eight STs were identified with 61.4% (35/57) belonging to ST-1. Furthermore, 14 AMR-associated genes and 76 virulence-associated genes were identified, with little genetic diversity between isolates belonging to the same ST. Several genes including <i>merR1</i> which is thought to play a role in ciprofloxacin-resistance were found to be significantly overrepresented in isolates sampled from herds using ampicillin/cloxacillin dry cow therapy. Overall, the presence of resistance genes remains low and current antimicrobial usage patterns do not appear to be driving AMR in <i>S. aureus</i> associated with bovine mastitis. |
| first_indexed | 2024-03-10T04:59:03Z |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T04:59:03Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-4b927c8c312d4ffba56a7b5aa4f61c8f2023-11-23T01:54:54ZengMDPI AGVeterinary Sciences2306-73812021-11-0181128710.3390/vetsci8110287The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy CattleSabrina S. Greening0Ji Zhang1Anne C. Midwinter2David A. Wilkinson3Scott McDougall4M. Carolyn Gates5Nigel P. French6School of Veterinary Science, Massey University, Palmerston North 4410, New ZealandInfectious Disease Research Centre, Massey University, Palmerston North 4410, New ZealandInfectious Disease Research Centre, Massey University, Palmerston North 4410, New ZealandInfectious Disease Research Centre, Massey University, Palmerston North 4410, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4410, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4410, New ZealandInfectious Disease Research Centre, Massey University, Palmerston North 4410, New Zealand<i>Staphylococcus aureus</i> is one of the leading causes of bovine mastitis worldwide and is a common indication for use of antimicrobials on dairy farms. This study aims to investigate the association between on-farm antimicrobial usage and the antimicrobial resistance (AMR) profiles of mastitis-causing <i>S. aureus</i>. Whole-genome sequencing was performed on 57 <i>S. aureus</i> isolates derived from cows with either clinical or subclinical mastitis from 17 dairy herds in New Zealand. The genetic relatedness between isolates was examined using the core single nucleotide polymorphism alignment whilst AMR and virulence genes were identified <i>in-silico</i>. The association between gene presence-absence and sequence type (ST), antimicrobial susceptibility and dry cow therapy treatment was investigated using Scoary. Altogether, eight STs were identified with 61.4% (35/57) belonging to ST-1. Furthermore, 14 AMR-associated genes and 76 virulence-associated genes were identified, with little genetic diversity between isolates belonging to the same ST. Several genes including <i>merR1</i> which is thought to play a role in ciprofloxacin-resistance were found to be significantly overrepresented in isolates sampled from herds using ampicillin/cloxacillin dry cow therapy. Overall, the presence of resistance genes remains low and current antimicrobial usage patterns do not appear to be driving AMR in <i>S. aureus</i> associated with bovine mastitis.https://www.mdpi.com/2306-7381/8/11/287<i>Staphylococcus aureus</i>mastitiswhole-genome sequencingantimicrobial resistancecattle |
| spellingShingle | Sabrina S. Greening Ji Zhang Anne C. Midwinter David A. Wilkinson Scott McDougall M. Carolyn Gates Nigel P. French The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle Veterinary Sciences <i>Staphylococcus aureus</i> mastitis whole-genome sequencing antimicrobial resistance cattle |
| title | The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle |
| title_full | The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle |
| title_fullStr | The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle |
| title_full_unstemmed | The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle |
| title_short | The Genetic Relatedness and Antimicrobial Resistance Patterns of Mastitis-Causing <i>Staphylococcus aureus</i> Strains Isolated from New Zealand Dairy Cattle |
| title_sort | genetic relatedness and antimicrobial resistance patterns of mastitis causing i staphylococcus aureus i strains isolated from new zealand dairy cattle |
| topic | <i>Staphylococcus aureus</i> mastitis whole-genome sequencing antimicrobial resistance cattle |
| url | https://www.mdpi.com/2306-7381/8/11/287 |
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