ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern China
Plums (Prunus spp.) are important deciduous fruit crops in the world. China is a major producer of P. salicina Lindl., but the genetic relationship of Chinese plums in key production regions remain unclear. In this study, 14 University of British Columbia (UBC) inter simple sequence repeats (ISSR) p...
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MDPI AG
2018-12-01
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| Series: | Biology |
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| Online Access: | http://www.mdpi.com/2079-7737/8/1/2 |
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| author | Weifeng Wu Faxing Chen Kaiwun Yeh Jianjun Chen |
| author_facet | Weifeng Wu Faxing Chen Kaiwun Yeh Jianjun Chen |
| author_sort | Weifeng Wu |
| collection | DOAJ |
| description | Plums (Prunus spp.) are important deciduous fruit crops in the world. China is a major producer of P. salicina Lindl., but the genetic relationship of Chinese plums in key production regions remain unclear. In this study, 14 University of British Columbia (UBC) inter simple sequence repeats (ISSR) primers were used to analyze 33 plum varieties cultivated in Fujian Province to determine their genetic diversity and population structure. A total of 146 bands were generated, of which 130 were polymorphic. Mean percentage of polymorphic bands was 89.04%, Shannon’s information index value was 0.38, and the Nei’s genetic index value was 0.24. Using unrooted trees (Neighbor-Joining method), 33 varieties were classified into four groups. Split graph separated them into two major groups, each with two subgroups. The two phylogenetic trees indicate that environmental or natural selection pressure is an important factor influencing their genetic relationship. Analysis of population structure revealed that they have frequent genetic exchanges among closed subpopulations; thus, genetic variation mainly occurs within the population. Additionally, based on the phylogenetic analysis and unique morphological characteristics of fruits, we propose that the Chinese landrace Nai could contribute significantly to development of the famous variety Wickson. |
| first_indexed | 2024-03-12T19:23:42Z |
| format | Article |
| id | doaj.art-4b92bb33f7d340c096de2c9dd07cd0aa |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T19:23:42Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-4b92bb33f7d340c096de2c9dd07cd0aa2023-08-02T04:57:42ZengMDPI AGBiology2079-77372018-12-0181210.3390/biology8010002biology8010002ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern ChinaWeifeng Wu0Faxing Chen1Kaiwun Yeh2Jianjun Chen3Institute of Subtropical Fruit, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Subtropical Fruit, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Subtropical Fruit, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaMid-Florida Research and Education Center, University of Florida, IFAS, Apopka, FL 32703, USAPlums (Prunus spp.) are important deciduous fruit crops in the world. China is a major producer of P. salicina Lindl., but the genetic relationship of Chinese plums in key production regions remain unclear. In this study, 14 University of British Columbia (UBC) inter simple sequence repeats (ISSR) primers were used to analyze 33 plum varieties cultivated in Fujian Province to determine their genetic diversity and population structure. A total of 146 bands were generated, of which 130 were polymorphic. Mean percentage of polymorphic bands was 89.04%, Shannon’s information index value was 0.38, and the Nei’s genetic index value was 0.24. Using unrooted trees (Neighbor-Joining method), 33 varieties were classified into four groups. Split graph separated them into two major groups, each with two subgroups. The two phylogenetic trees indicate that environmental or natural selection pressure is an important factor influencing their genetic relationship. Analysis of population structure revealed that they have frequent genetic exchanges among closed subpopulations; thus, genetic variation mainly occurs within the population. Additionally, based on the phylogenetic analysis and unique morphological characteristics of fruits, we propose that the Chinese landrace Nai could contribute significantly to development of the famous variety Wickson.http://www.mdpi.com/2079-7737/8/1/2Chinese plumsgenetic diversitygenetic structurePrunus salicinasimple-sequence repeat markers (ISSR)plum |
| spellingShingle | Weifeng Wu Faxing Chen Kaiwun Yeh Jianjun Chen ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern China Biology Chinese plums genetic diversity genetic structure Prunus salicina simple-sequence repeat markers (ISSR) plum |
| title | ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern China |
| title_full | ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern China |
| title_fullStr | ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern China |
| title_full_unstemmed | ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern China |
| title_short | ISSR Analysis of Genetic Diversity and Structure of Plum Varieties Cultivated in Southern China |
| title_sort | issr analysis of genetic diversity and structure of plum varieties cultivated in southern china |
| topic | Chinese plums genetic diversity genetic structure Prunus salicina simple-sequence repeat markers (ISSR) plum |
| url | http://www.mdpi.com/2079-7737/8/1/2 |
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