Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry
Metagenomics and metatranscriptomics have enhanced our understanding of the oral microbiome and its impact on oral health. However, these approaches have inherent limitations in exploring individual cells and the heterogeneity within mixed microbial communities, which restricts our current understan...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Japanese Dental Science Review |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1882761623000212 |
| _version_ | 1797387861503246336 |
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| author | Ji-Hoi Moon Dae-Hyun Roh Kyu Hwan Kwack Jae-Hyung Lee |
| author_facet | Ji-Hoi Moon Dae-Hyun Roh Kyu Hwan Kwack Jae-Hyung Lee |
| author_sort | Ji-Hoi Moon |
| collection | DOAJ |
| description | Metagenomics and metatranscriptomics have enhanced our understanding of the oral microbiome and its impact on oral health. However, these approaches have inherent limitations in exploring individual cells and the heterogeneity within mixed microbial communities, which restricts our current understanding to bulk cells and species-level information. Fortunately, recent technical advances have enabled the application of single-cell RNA sequencing (scRNA-seq) for studying bacteria, shedding light on cell-to-cell diversity and interactions between host-bacterial cells at the single-cell level. Here, we address the technical barriers in capturing RNA from single bacterial cells and highlight pioneering studies from the past decade. We also discuss recent achievements in host-bacterial dual transcriptional profiling at the single-cell level. Bacterial scRNA-seq provides advantages in various research fields, including the investigation of phenotypic heterogeneity within genetically identical bacteria, identification of rare cell types, detection of antibiotic-resistant or persistent cells, analysis of individual gene expression patterns and metabolic activities, and characterization of specific microbe-host interactions. Integrating single-cell techniques with bulk approaches is essential to gain a comprehensive understanding of oral diseases and develop targeted and personalized treatment in dentistry. The reviewed pioneering studies are expected to inspire future research on the oral microbiome at the single-cell level. |
| first_indexed | 2024-03-08T22:31:13Z |
| format | Article |
| id | doaj.art-4b94a89b6eb744e2814bf499a58e07cb |
| institution | Directory Open Access Journal |
| issn | 1882-7616 |
| language | English |
| last_indexed | 2024-03-08T22:31:13Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Japanese Dental Science Review |
| spelling | doaj.art-4b94a89b6eb744e2814bf499a58e07cb2023-12-18T04:24:07ZengElsevierJapanese Dental Science Review1882-76162023-12-0159253262Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistryJi-Hoi Moon0Dae-Hyun Roh1Kyu Hwan Kwack2Jae-Hyung Lee3Department of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul, Republic of Korea; Correspondence to: Department of Oral Microbiology, College of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.Department of Oral Physiology, College of Dentistry, Kyung Hee University, Seoul, Republic of KoreaDepartment of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul, Republic of KoreaDepartment of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul, Republic of Korea; Correspondence to: Department of Oral Microbiology, College of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.Metagenomics and metatranscriptomics have enhanced our understanding of the oral microbiome and its impact on oral health. However, these approaches have inherent limitations in exploring individual cells and the heterogeneity within mixed microbial communities, which restricts our current understanding to bulk cells and species-level information. Fortunately, recent technical advances have enabled the application of single-cell RNA sequencing (scRNA-seq) for studying bacteria, shedding light on cell-to-cell diversity and interactions between host-bacterial cells at the single-cell level. Here, we address the technical barriers in capturing RNA from single bacterial cells and highlight pioneering studies from the past decade. We also discuss recent achievements in host-bacterial dual transcriptional profiling at the single-cell level. Bacterial scRNA-seq provides advantages in various research fields, including the investigation of phenotypic heterogeneity within genetically identical bacteria, identification of rare cell types, detection of antibiotic-resistant or persistent cells, analysis of individual gene expression patterns and metabolic activities, and characterization of specific microbe-host interactions. Integrating single-cell techniques with bulk approaches is essential to gain a comprehensive understanding of oral diseases and develop targeted and personalized treatment in dentistry. The reviewed pioneering studies are expected to inspire future research on the oral microbiome at the single-cell level.http://www.sciencedirect.com/science/article/pii/S1882761623000212Single-cellBacteriaTranscriptomicsscRNA-seq |
| spellingShingle | Ji-Hoi Moon Dae-Hyun Roh Kyu Hwan Kwack Jae-Hyung Lee Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry Japanese Dental Science Review Single-cell Bacteria Transcriptomics scRNA-seq |
| title | Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry |
| title_full | Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry |
| title_fullStr | Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry |
| title_full_unstemmed | Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry |
| title_short | Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry |
| title_sort | bacterial single cell transcriptomics recent technical advances and future applications in dentistry |
| topic | Single-cell Bacteria Transcriptomics scRNA-seq |
| url | http://www.sciencedirect.com/science/article/pii/S1882761623000212 |
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