Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway
Advanced glycation end products (AGE) are those of the most powerful pathogenic factors that related to diabetic complications. In our study, we investigated the beneficial effects of thymol on AGE induced cell injury and apoptosis in human podocytes (HPCs) and attempted to clarify its mechanisms. O...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Cell Adhesion & Migration |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19336918.2020.1721172 |
| _version_ | 1818427020600672256 |
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| author | Qinglian Wang Zhenwei Shen Guanghui Qi Yanfang Zhao Hongge Zhang Rong Wang |
| author_facet | Qinglian Wang Zhenwei Shen Guanghui Qi Yanfang Zhao Hongge Zhang Rong Wang |
| author_sort | Qinglian Wang |
| collection | DOAJ |
| description | Advanced glycation end products (AGE) are those of the most powerful pathogenic factors that related to diabetic complications. In our study, we investigated the beneficial effects of thymol on AGE induced cell injury and apoptosis in human podocytes (HPCs) and attempted to clarify its mechanisms. Our results revealed that stimulation with AGE could significantly activate RhoA/NF-κB pathway. Results showed thymol could markedly suppress inflammatory responses, cell apoptosis and disordered cytoskeleton. Also thymol restored the expression of podocin, restrained migration capacity. Western blot analysis indicated that it could restore the expression of RhoA, ROCK and vimentin, nephrin, podocin and p65 and IκBα phosphorylation. Moreover, si-RhoA also suppressed the expression of pro-inflammatory cytokines, ROCK, and vimentin and the phosphorylation of p65 and IκBα. In conclusion, thymol inhibits AGE-induced cell injury in HPCs by suppressing the RhoA-NF-κB pathway and may be apromising therapeutic agent. |
| first_indexed | 2024-12-14T14:39:05Z |
| format | Article |
| id | doaj.art-4b954de5be2744d3b7512dbb4e890d01 |
| institution | Directory Open Access Journal |
| issn | 1933-6918 1933-6926 |
| language | English |
| last_indexed | 2024-12-14T14:39:05Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj.art-4b954de5be2744d3b7512dbb4e890d012022-12-21T22:57:29ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262020-01-01141425610.1080/19336918.2020.17211721721172Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathwayQinglian Wang0Zhenwei Shen1Guanghui Qi2Yanfang Zhao3Hongge Zhang4Rong Wang5Shandong Provincial Hospital Affiliated to Shandong UniversityWuXi Clinical Development Service (Shanghai) Co., LtdThe First Hospital of ZiboShandong Provincial Hospital Affiliated to Shandong UniversityTengzhou Hospital of Traditional Chinese MedicineShandong Provincial Hospital Affiliated to Shandong UniversityAdvanced glycation end products (AGE) are those of the most powerful pathogenic factors that related to diabetic complications. In our study, we investigated the beneficial effects of thymol on AGE induced cell injury and apoptosis in human podocytes (HPCs) and attempted to clarify its mechanisms. Our results revealed that stimulation with AGE could significantly activate RhoA/NF-κB pathway. Results showed thymol could markedly suppress inflammatory responses, cell apoptosis and disordered cytoskeleton. Also thymol restored the expression of podocin, restrained migration capacity. Western blot analysis indicated that it could restore the expression of RhoA, ROCK and vimentin, nephrin, podocin and p65 and IκBα phosphorylation. Moreover, si-RhoA also suppressed the expression of pro-inflammatory cytokines, ROCK, and vimentin and the phosphorylation of p65 and IκBα. In conclusion, thymol inhibits AGE-induced cell injury in HPCs by suppressing the RhoA-NF-κB pathway and may be apromising therapeutic agent.http://dx.doi.org/10.1080/19336918.2020.1721172thymoladvanced glycation end products (age)human podocytesrhoa/rock pathwaynuclear factor-kappa b (nf-κb) |
| spellingShingle | Qinglian Wang Zhenwei Shen Guanghui Qi Yanfang Zhao Hongge Zhang Rong Wang Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway Cell Adhesion & Migration thymol advanced glycation end products (age) human podocytes rhoa/rock pathway nuclear factor-kappa b (nf-κb) |
| title | Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway |
| title_full | Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway |
| title_fullStr | Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway |
| title_full_unstemmed | Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway |
| title_short | Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via NF-κB-mediated by RhoA/ROCK signalling pathway |
| title_sort | thymol alleviates ages induced podocyte injury by a pleiotropic effect via nf κb mediated by rhoa rock signalling pathway |
| topic | thymol advanced glycation end products (age) human podocytes rhoa/rock pathway nuclear factor-kappa b (nf-κb) |
| url | http://dx.doi.org/10.1080/19336918.2020.1721172 |
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