Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells.
Icaritin (ICT) is a hydrolytic form of icariin isolated from plants of the genus Epimedium. This study was to investigate the radiosensitization effect of icaritin and its possible underlying mechanism using murine 4T1 breast cancer cells. The combination of Icaritin at 3 µM or 6 µM with 6 or 8 Gy o...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3744569?pdf=render |
| _version_ | 1818897171434438656 |
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| author | Jinsheng Hong Zhenhuan Zhang Wenlong Lv Mei Zhang Chun Chen Shanmin Yang Shan Li Lurong Zhang Deping Han Weijian Zhang |
| author_facet | Jinsheng Hong Zhenhuan Zhang Wenlong Lv Mei Zhang Chun Chen Shanmin Yang Shan Li Lurong Zhang Deping Han Weijian Zhang |
| author_sort | Jinsheng Hong |
| collection | DOAJ |
| description | Icaritin (ICT) is a hydrolytic form of icariin isolated from plants of the genus Epimedium. This study was to investigate the radiosensitization effect of icaritin and its possible underlying mechanism using murine 4T1 breast cancer cells. The combination of Icaritin at 3 µM or 6 µM with 6 or 8 Gy of ionizing radiation (IR) in the clonogenic assay yielded an ER (enhancement ratio) of 1.18 or 1.28, CI (combination index) of 0.38 or 0.19 and DRI (dose reducing index) of 2.51 or 5.07, respectively. These strongly suggest that Icaritin exerted a synergistic killing (?) effect with radiation on the tumor cells. This effect might relate with bioactivities of ICT: 1) exert an anti-proliferative effect in a dose- and time-dependent manner, which is different from IR killing effect but likely work together with the IR effect; 2) suppress the IR-induced activation of two survival paths, ERK1/2 and AKT; 3) induce the G2/M blockage, enhancing IR killing effect; and 4) synergize with IR to enhance cell apoptosis. In addition, ICT suppressed angiogenesis in chick embryo chorioallantoic membrane (CAM) assay. Taken together, ICT is a new radiosensitizer and can enhance anti-cancer effect of IR or other therapies. |
| first_indexed | 2024-12-19T19:11:56Z |
| format | Article |
| id | doaj.art-4b9987645d5c4fd4803948619d0624a5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T19:11:56Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4b9987645d5c4fd4803948619d0624a52022-12-21T20:09:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7134710.1371/journal.pone.0071347Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells.Jinsheng HongZhenhuan ZhangWenlong LvMei ZhangChun ChenShanmin YangShan LiLurong ZhangDeping HanWeijian ZhangIcaritin (ICT) is a hydrolytic form of icariin isolated from plants of the genus Epimedium. This study was to investigate the radiosensitization effect of icaritin and its possible underlying mechanism using murine 4T1 breast cancer cells. The combination of Icaritin at 3 µM or 6 µM with 6 or 8 Gy of ionizing radiation (IR) in the clonogenic assay yielded an ER (enhancement ratio) of 1.18 or 1.28, CI (combination index) of 0.38 or 0.19 and DRI (dose reducing index) of 2.51 or 5.07, respectively. These strongly suggest that Icaritin exerted a synergistic killing (?) effect with radiation on the tumor cells. This effect might relate with bioactivities of ICT: 1) exert an anti-proliferative effect in a dose- and time-dependent manner, which is different from IR killing effect but likely work together with the IR effect; 2) suppress the IR-induced activation of two survival paths, ERK1/2 and AKT; 3) induce the G2/M blockage, enhancing IR killing effect; and 4) synergize with IR to enhance cell apoptosis. In addition, ICT suppressed angiogenesis in chick embryo chorioallantoic membrane (CAM) assay. Taken together, ICT is a new radiosensitizer and can enhance anti-cancer effect of IR or other therapies.http://europepmc.org/articles/PMC3744569?pdf=render |
| spellingShingle | Jinsheng Hong Zhenhuan Zhang Wenlong Lv Mei Zhang Chun Chen Shanmin Yang Shan Li Lurong Zhang Deping Han Weijian Zhang Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells. PLoS ONE |
| title | Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells. |
| title_full | Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells. |
| title_fullStr | Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells. |
| title_full_unstemmed | Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells. |
| title_short | Icaritin synergistically enhances the radiosensitivity of 4T1 breast cancer cells. |
| title_sort | icaritin synergistically enhances the radiosensitivity of 4t1 breast cancer cells |
| url | http://europepmc.org/articles/PMC3744569?pdf=render |
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