Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells
Reagents to monitor T cell responses to the entire HIV genome, based on well characterized epitopes, are missing. Evaluation of HIV-specific T cell responses is of importance to study natural infection, and therapeutic and vaccine interventions. Experimentally derived CD4+ and CD8+ HIV epitopes from...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9380920/?tool=EBI |
| _version_ | 1811340787332415488 |
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| author | Rita Al-kolla Alba Grifoni Shane Crotty Alessandro Sette Sara Gianella Jennifer Dan |
| author_facet | Rita Al-kolla Alba Grifoni Shane Crotty Alessandro Sette Sara Gianella Jennifer Dan |
| author_sort | Rita Al-kolla |
| collection | DOAJ |
| description | Reagents to monitor T cell responses to the entire HIV genome, based on well characterized epitopes, are missing. Evaluation of HIV-specific T cell responses is of importance to study natural infection, and therapeutic and vaccine interventions. Experimentally derived CD4+ and CD8+ HIV epitopes from the HIV molecular immunology database were developed into Class I and Class II HIV megapools (MPs). We assessed HIV responses in persons with HIV pre antiretroviral therapy (ART) (n = 17) and post-ART (n = 18) and compared these responses to 15 controls without HIV (matched by sex at birth, age, and ethnicity). Using the Activation Induced Marker (AIM) assay, we quantified HIV-specific total CD4+, memory CD4+, circulating T follicular helper, total CD8+ and memory CD8+ T cells. We also compared the Class I and Class II HIV MPs to commercially available HIV gag peptide pools. Overall, HIV Class II MP detected HIV-specific CD4+ T cells in 21/35 (60%) HIV positive samples and 0/15 HIV negative samples. HIV Class I MP detected an HIV-specific CD8+ T cells in 17/35 (48.6%) HIV positive samples and 0/15 HIV negative samples. Our innovative HIV MPs are reflective of the entire HIV genome, and its performance is comparable to other commercially available peptide pools. Here, we detected HIV-specific CD4+ and CD8+ T cell responses in people on and off ART, but not in people without HIV. |
| first_indexed | 2024-04-13T18:47:09Z |
| format | Article |
| id | doaj.art-4b9c2dfade984da7a3624b0314bcd1b4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T18:47:09Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4b9c2dfade984da7a3624b0314bcd1b42022-12-22T02:34:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01178Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cellsRita Al-kollaAlba GrifoniShane CrottyAlessandro SetteSara GianellaJennifer DanReagents to monitor T cell responses to the entire HIV genome, based on well characterized epitopes, are missing. Evaluation of HIV-specific T cell responses is of importance to study natural infection, and therapeutic and vaccine interventions. Experimentally derived CD4+ and CD8+ HIV epitopes from the HIV molecular immunology database were developed into Class I and Class II HIV megapools (MPs). We assessed HIV responses in persons with HIV pre antiretroviral therapy (ART) (n = 17) and post-ART (n = 18) and compared these responses to 15 controls without HIV (matched by sex at birth, age, and ethnicity). Using the Activation Induced Marker (AIM) assay, we quantified HIV-specific total CD4+, memory CD4+, circulating T follicular helper, total CD8+ and memory CD8+ T cells. We also compared the Class I and Class II HIV MPs to commercially available HIV gag peptide pools. Overall, HIV Class II MP detected HIV-specific CD4+ T cells in 21/35 (60%) HIV positive samples and 0/15 HIV negative samples. HIV Class I MP detected an HIV-specific CD8+ T cells in 17/35 (48.6%) HIV positive samples and 0/15 HIV negative samples. Our innovative HIV MPs are reflective of the entire HIV genome, and its performance is comparable to other commercially available peptide pools. Here, we detected HIV-specific CD4+ and CD8+ T cell responses in people on and off ART, but not in people without HIV.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9380920/?tool=EBI |
| spellingShingle | Rita Al-kolla Alba Grifoni Shane Crotty Alessandro Sette Sara Gianella Jennifer Dan Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells PLoS ONE |
| title | Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells |
| title_full | Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells |
| title_fullStr | Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells |
| title_full_unstemmed | Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells |
| title_short | Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells |
| title_sort | design and validation of hiv peptide pools for detection of hiv specific cd4 and cd8 t cells |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9380920/?tool=EBI |
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