Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16
Abstract Objectives This research aimed to examine the antitumor mechanisms of selenium nanoparticles (SeNPs) specifically against prostate cancers. Methods The antitumor activities of SeNPs against cancer cells were determined via MTT assay. The cell cycle was determined by detecting the DNA conten...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12957-020-01850-7 |
| _version_ | 1819038922457481216 |
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| author | Guolong Liao Jiani Tang Di Wang Haoru Zuo Qi Zhang Ying Liu Haiyun Xiong |
| author_facet | Guolong Liao Jiani Tang Di Wang Haoru Zuo Qi Zhang Ying Liu Haiyun Xiong |
| author_sort | Guolong Liao |
| collection | DOAJ |
| description | Abstract Objectives This research aimed to examine the antitumor mechanisms of selenium nanoparticles (SeNPs) specifically against prostate cancers. Methods The antitumor activities of SeNPs against cancer cells were determined via MTT assay. The cell cycle was determined by detecting the DNA content, and apoptosis was determined via annexin V-Fluos staining kit. The microRNA expressions in cancer cells were analyzed via microarray and qRT-PCR. The potential targets of miR-16 were identified via luciferase analysis and mRNA expression determination. miR-16 functions in cancer cells were explored via the transient transfection of miR-16 mimic or inhibitor. Results SeNPs were most potent in prostate cancer cells, regardless of whether or not they were androgen-dependent. Furthermore, SeNP stimulation can induce cell cycle arrest and the apoptosis enhancement of prostate cancer cells. Microarray and molecular mechanism studies demonstrated that miR-16 could directly target cyclin D1 and BCL-2 to mediate SeNP apoptosis enhancement. Results show that the serum selenium levels positively correlate with miR-16 expressions, and they correlate with the overall and disease-free survival rates. Conclusion These results signify the cytotoxic potential of SeNPs in prostate cancer treatment. |
| first_indexed | 2024-12-21T08:45:00Z |
| format | Article |
| id | doaj.art-4b9c3a1235e647018b987fac562baa2c |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-21T08:45:00Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-4b9c3a1235e647018b987fac562baa2c2022-12-21T19:09:50ZengBMCWorld Journal of Surgical Oncology1477-78192020-05-0118111110.1186/s12957-020-01850-7Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16Guolong Liao0Jiani Tang1Di Wang2Haoru Zuo3Qi Zhang4Ying Liu5Haiyun Xiong6Department of Urology, The Seventh Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Urology, The Seventh Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Clinical Laboratory, PLA 309 HospitalDepartment of Surgery Anesthesia Center, the Seventh Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Surgery Anesthesia Center, the Seventh Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Surgery Anesthesia Center, the Seventh Affiliated Hospital of Sun Yat-Sen UniversityDepartment of Urology, The Seventh Affiliated Hospital of Sun Yat-Sen UniversityAbstract Objectives This research aimed to examine the antitumor mechanisms of selenium nanoparticles (SeNPs) specifically against prostate cancers. Methods The antitumor activities of SeNPs against cancer cells were determined via MTT assay. The cell cycle was determined by detecting the DNA content, and apoptosis was determined via annexin V-Fluos staining kit. The microRNA expressions in cancer cells were analyzed via microarray and qRT-PCR. The potential targets of miR-16 were identified via luciferase analysis and mRNA expression determination. miR-16 functions in cancer cells were explored via the transient transfection of miR-16 mimic or inhibitor. Results SeNPs were most potent in prostate cancer cells, regardless of whether or not they were androgen-dependent. Furthermore, SeNP stimulation can induce cell cycle arrest and the apoptosis enhancement of prostate cancer cells. Microarray and molecular mechanism studies demonstrated that miR-16 could directly target cyclin D1 and BCL-2 to mediate SeNP apoptosis enhancement. Results show that the serum selenium levels positively correlate with miR-16 expressions, and they correlate with the overall and disease-free survival rates. Conclusion These results signify the cytotoxic potential of SeNPs in prostate cancer treatment.http://link.springer.com/article/10.1186/s12957-020-01850-7Selenium nanoparticlesProstate cancerCell cycleApoptosisMicroRNA |
| spellingShingle | Guolong Liao Jiani Tang Di Wang Haoru Zuo Qi Zhang Ying Liu Haiyun Xiong Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16 World Journal of Surgical Oncology Selenium nanoparticles Prostate cancer Cell cycle Apoptosis MicroRNA |
| title | Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16 |
| title_full | Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16 |
| title_fullStr | Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16 |
| title_full_unstemmed | Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16 |
| title_short | Selenium nanoparticles (SeNPs) have potent antitumor activity against prostate cancer cells through the upregulation of miR-16 |
| title_sort | selenium nanoparticles senps have potent antitumor activity against prostate cancer cells through the upregulation of mir 16 |
| topic | Selenium nanoparticles Prostate cancer Cell cycle Apoptosis MicroRNA |
| url | http://link.springer.com/article/10.1186/s12957-020-01850-7 |
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