Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay
Porcine parvovirus (PPV) is widely prevalent in pig farms. PPV is closely related to porcine respiratory disease complex (PRDC) and porcine circovirus disease (PCVD), which seriously threatens the healthy development of the pig industry. Although commercial antibody detection kits are available, the...
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MDPI AG
2022-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1828 |
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| author | Yanfei Gao Haiwei Wang Shanghui Wang Mingxia Sun Zheng Fang Xinran Liu Xuehui Cai Yabin Tu |
| author_facet | Yanfei Gao Haiwei Wang Shanghui Wang Mingxia Sun Zheng Fang Xinran Liu Xuehui Cai Yabin Tu |
| author_sort | Yanfei Gao |
| collection | DOAJ |
| description | Porcine parvovirus (PPV) is widely prevalent in pig farms. PPV is closely related to porcine respiratory disease complex (PRDC) and porcine circovirus disease (PCVD), which seriously threatens the healthy development of the pig industry. Although commercial antibody detection kits are available, they are expensive and unsuitable for large-scale clinical practice. Here, a soluble VP2 protein of PPV is efficiently expressed in the <i>E. coli</i> expression system. The VP2 protein can be self-assembled into virus-like particles (VLPs) in vitro. After multiple steps of chromatography purification, PPV-VLPs with a purity of about 95% were obtained. An indirect, enzyme-linked immunosorbent assay (I-ELISA), comparable to a commercial PPV kit, was developed based on the purified PPV-VLPs and was used to detect 487 clinical pig serum samples. The results showed that the I-ELISA is a simple, cost-effective, and efficient method for the diagnosis of clinical pig serum and plasma samples. In summary, high-purity, tag-free PPV-VLPs were prepared, and the established VLP-based I-ELISA is of great significance for the sero-monitoring of antibodies against PPV. |
| first_indexed | 2024-03-09T03:41:21Z |
| format | Article |
| id | doaj.art-4b9cbecb08344bce9771cffe2ceaf699 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T03:41:21Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4b9cbecb08344bce9771cffe2ceaf6992023-12-03T14:39:53ZengMDPI AGViruses1999-49152022-08-01148182810.3390/v14081828Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological AssayYanfei Gao0Haiwei Wang1Shanghui Wang2Mingxia Sun3Zheng Fang4Xinran Liu5Xuehui Cai6Yabin Tu7State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaRegeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York, NY 10591, USAState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, ChinaPorcine parvovirus (PPV) is widely prevalent in pig farms. PPV is closely related to porcine respiratory disease complex (PRDC) and porcine circovirus disease (PCVD), which seriously threatens the healthy development of the pig industry. Although commercial antibody detection kits are available, they are expensive and unsuitable for large-scale clinical practice. Here, a soluble VP2 protein of PPV is efficiently expressed in the <i>E. coli</i> expression system. The VP2 protein can be self-assembled into virus-like particles (VLPs) in vitro. After multiple steps of chromatography purification, PPV-VLPs with a purity of about 95% were obtained. An indirect, enzyme-linked immunosorbent assay (I-ELISA), comparable to a commercial PPV kit, was developed based on the purified PPV-VLPs and was used to detect 487 clinical pig serum samples. The results showed that the I-ELISA is a simple, cost-effective, and efficient method for the diagnosis of clinical pig serum and plasma samples. In summary, high-purity, tag-free PPV-VLPs were prepared, and the established VLP-based I-ELISA is of great significance for the sero-monitoring of antibodies against PPV.https://www.mdpi.com/1999-4915/14/8/1828porcine parvovirusvirus-like particlesdiagnosticI-ELISA |
| spellingShingle | Yanfei Gao Haiwei Wang Shanghui Wang Mingxia Sun Zheng Fang Xinran Liu Xuehui Cai Yabin Tu Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay Viruses porcine parvovirus virus-like particles diagnostic I-ELISA |
| title | Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay |
| title_full | Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay |
| title_fullStr | Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay |
| title_full_unstemmed | Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay |
| title_short | Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay |
| title_sort | self assembly of porcine parvovirus virus like particles and their application in serological assay |
| topic | porcine parvovirus virus-like particles diagnostic I-ELISA |
| url | https://www.mdpi.com/1999-4915/14/8/1828 |
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