Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin
Colistin is considered as an antibiotic of ‘last resort’ for the treatment of lethal infections caused by carbapenem-resistant Enterobacterales (CRE), dissemination of plasmid-borne colistin resistance gene mcr-1, particularly into CRE, resulting in the emergence of strains that approach pan-resista...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.950087/full |
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| author | Shuang Xia Wei Wang Jing Cheng Jing Cheng Tingting Zhang Tingting Zhang Ziwei Xia Ziwei Xia Xiaoyu Zhao Xiaoyu Zhao Yungang Han Yonghong Li Yonghong Li Xiufang Shi Xiufang Shi Shangshang Qin Shangshang Qin |
| author_facet | Shuang Xia Wei Wang Jing Cheng Jing Cheng Tingting Zhang Tingting Zhang Ziwei Xia Ziwei Xia Xiaoyu Zhao Xiaoyu Zhao Yungang Han Yonghong Li Yonghong Li Xiufang Shi Xiufang Shi Shangshang Qin Shangshang Qin |
| author_sort | Shuang Xia |
| collection | DOAJ |
| description | Colistin is considered as an antibiotic of ‘last resort’ for the treatment of lethal infections caused by carbapenem-resistant Enterobacterales (CRE), dissemination of plasmid-borne colistin resistance gene mcr-1, particularly into CRE, resulting in the emergence of strains that approach pan-resistance. A wide variety of plasmid types have been reported for carrying mcr-1. Among which, large IncHI2-type plasmids were multidrug-resistant (MDR) plasmids harbored multiple resistance determinants in addition to mcr-1. Herein, we characterized a novel hybrid IncHI2-like mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain EC15-50 of clinical origin. Antimicrobial susceptibility testing showed E. coli EC15-50 exhibited an extensively drug-resistant (XDR) profile that only susceptible to amikacin and tigecycline. S1-PFGE, Southern hybridization and Whole-genome Sequencing (WGS) analysis identified a 46,161 bp blaNDM-7-harboring IncX3 plasmid pEC50-NDM7 and a 350,179 bp mcr-1-bearing IncHI2/HI2A/N/FII/FIA plasmid pEC15-MCR-50 in E. coli EC15-50. Sequence detail analysis revealed the type IV coupling protein (T4CP) gene was absent on pEC15-MCR-50, explaining that pEC15-MCR-50 was a non-conjugative plasmid. Comparative genetic analysis indicated the hybrid plasmid pEC15-MCR-50 was probably originated from pXGE1mcr-like IncHI2/HI2A/N plasmid and pSJ_94-like IncFII/FIA plasmid, and generated as a result of a replicative transposition process mediated by IS26. Currently, the prevalent mcr-1-carrying IncHI2 plasmids were rarely reported to be fused with other plasmids. The identification of the novel hybrid plasmid pEC15-MCR-50 in this study highlighted the importance of close surveillance for the emergence and dissemination of such fusion MDR plasmids, particularly in NDM-producing Enterobacterales. |
| first_indexed | 2024-04-11T21:20:56Z |
| format | Article |
| id | doaj.art-4ba0a33bdca84c59b6e3d55497ff2bc1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T21:20:56Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4ba0a33bdca84c59b6e3d55497ff2bc12022-12-22T04:02:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.950087950087Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical originShuang Xia0Wei Wang1Jing Cheng2Jing Cheng3Tingting Zhang4Tingting Zhang5Ziwei Xia6Ziwei Xia7Xiaoyu Zhao8Xiaoyu Zhao9Yungang Han10Yonghong Li11Yonghong Li12Xiufang Shi13Xiufang Shi14Shangshang Qin15Shangshang Qin16Department of Medical Laboratory, Henan Provincial Chest Hospital, Zhengzhou, ChinaDepartment of Medical Laboratory, Henan Provincial Chest Hospital, Zhengzhou, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, ChinaKey Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, ChinaKey Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, ChinaKey Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, ChinaKey Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, ChinaDepartment of Medical Laboratory, Henan Provincial Chest Hospital, Zhengzhou, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, ChinaKey Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, ChinaKey Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, ChinaKey Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, ChinaColistin is considered as an antibiotic of ‘last resort’ for the treatment of lethal infections caused by carbapenem-resistant Enterobacterales (CRE), dissemination of plasmid-borne colistin resistance gene mcr-1, particularly into CRE, resulting in the emergence of strains that approach pan-resistance. A wide variety of plasmid types have been reported for carrying mcr-1. Among which, large IncHI2-type plasmids were multidrug-resistant (MDR) plasmids harbored multiple resistance determinants in addition to mcr-1. Herein, we characterized a novel hybrid IncHI2-like mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain EC15-50 of clinical origin. Antimicrobial susceptibility testing showed E. coli EC15-50 exhibited an extensively drug-resistant (XDR) profile that only susceptible to amikacin and tigecycline. S1-PFGE, Southern hybridization and Whole-genome Sequencing (WGS) analysis identified a 46,161 bp blaNDM-7-harboring IncX3 plasmid pEC50-NDM7 and a 350,179 bp mcr-1-bearing IncHI2/HI2A/N/FII/FIA plasmid pEC15-MCR-50 in E. coli EC15-50. Sequence detail analysis revealed the type IV coupling protein (T4CP) gene was absent on pEC15-MCR-50, explaining that pEC15-MCR-50 was a non-conjugative plasmid. Comparative genetic analysis indicated the hybrid plasmid pEC15-MCR-50 was probably originated from pXGE1mcr-like IncHI2/HI2A/N plasmid and pSJ_94-like IncFII/FIA plasmid, and generated as a result of a replicative transposition process mediated by IS26. Currently, the prevalent mcr-1-carrying IncHI2 plasmids were rarely reported to be fused with other plasmids. The identification of the novel hybrid plasmid pEC15-MCR-50 in this study highlighted the importance of close surveillance for the emergence and dissemination of such fusion MDR plasmids, particularly in NDM-producing Enterobacterales.https://www.frontiersin.org/articles/10.3389/fmicb.2022.950087/fullEscherichia coliST167mcr-1cointegrate plasmidIs26 |
| spellingShingle | Shuang Xia Wei Wang Jing Cheng Jing Cheng Tingting Zhang Tingting Zhang Ziwei Xia Ziwei Xia Xiaoyu Zhao Xiaoyu Zhao Yungang Han Yonghong Li Yonghong Li Xiufang Shi Xiufang Shi Shangshang Qin Shangshang Qin Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin Frontiers in Microbiology Escherichia coli ST167 mcr-1 cointegrate plasmid Is26 |
| title | Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin |
| title_full | Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin |
| title_fullStr | Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin |
| title_full_unstemmed | Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin |
| title_short | Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin |
| title_sort | emergence of a novel hybrid mcr 1 bearing plasmid in an ndm 7 producing st167 escherichia coli strain of clinical origin |
| topic | Escherichia coli ST167 mcr-1 cointegrate plasmid Is26 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.950087/full |
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