Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan
Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leukosis (EBL) and has worldwide distribution. Infections with BLV have been reported in cattle from Kazakhstan but the virus has not yet been thoroughly characterized. In this study, we detect and estimate the level of BLV prov...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/11/2/180 |
| _version_ | 1827653360330735616 |
|---|---|
| author | Akhmetzhan Sultanov Marzena Rola-Łuszczak Saltanat Mamanova Anna Ryło Zbigniew Osiński Meruyert A. Saduakassova Elvira Bashenova Jacek Kuźmak |
| author_facet | Akhmetzhan Sultanov Marzena Rola-Łuszczak Saltanat Mamanova Anna Ryło Zbigniew Osiński Meruyert A. Saduakassova Elvira Bashenova Jacek Kuźmak |
| author_sort | Akhmetzhan Sultanov |
| collection | DOAJ |
| description | Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leukosis (EBL) and has worldwide distribution. Infections with BLV have been reported in cattle from Kazakhstan but the virus has not yet been thoroughly characterized. In this study, we detect and estimate the level of BLV proviral DNA by qPCR in DNA samples from 119 cattle naturally infected with BLV, from 18 farms located in four different geographical regions of Kazakhstan. Furthermore, we conducted the phylogenetic and molecular analysis of 41 BLV env-gp51 gene sequences from BLV infected cattle. Phylogenetic analysis showed the affiliation of sequences to two already known genotypes G4 and G7 and also to a new genotype, classified as genotype G12. In addition, a multivariate method was employed for analysis of the association between proviral load and different variables such as the geographical location of the herd, cattle breeds, age of animals, and the presence of particular BLV genotypes. In summary, the results of this study provide the first evidence on molecular characterization of BLV circulating in cattle from Kazakhstan. |
| first_indexed | 2024-03-09T21:16:49Z |
| format | Article |
| id | doaj.art-4ba5511d972944f98d35a9a41d817f1c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T21:16:49Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-4ba5511d972944f98d35a9a41d817f1c2023-11-23T21:31:37ZengMDPI AGPathogens2076-08172022-01-0111218010.3390/pathogens11020180Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from KazakhstanAkhmetzhan Sultanov0Marzena Rola-Łuszczak1Saltanat Mamanova2Anna Ryło3Zbigniew Osiński4Meruyert A. Saduakassova5Elvira Bashenova6Jacek Kuźmak7The Kazakh Scientific-Research Veterinary Institute, Almaty 050016, KazakhstanDepartment of Biochemistry, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment for Epizootological Monitoring and Risks Assessment of Animal Viral Diseases, The Kazakh Scientific-Research Veterinary Institute, Almaty 050016, KazakhstanDepartment of Biochemistry, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment for Epizootological Monitoring and Risks Assessment of Animal Viral Diseases, The Kazakh Scientific-Research Veterinary Institute, Almaty 050016, KazakhstanDepartment for Epizootological Monitoring and Risks Assessment of Animal Viral Diseases, The Kazakh Scientific-Research Veterinary Institute, Almaty 050016, KazakhstanDepartment of Biochemistry, National Veterinary Research Institute, 24-100 Puławy, PolandBovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leukosis (EBL) and has worldwide distribution. Infections with BLV have been reported in cattle from Kazakhstan but the virus has not yet been thoroughly characterized. In this study, we detect and estimate the level of BLV proviral DNA by qPCR in DNA samples from 119 cattle naturally infected with BLV, from 18 farms located in four different geographical regions of Kazakhstan. Furthermore, we conducted the phylogenetic and molecular analysis of 41 BLV env-gp51 gene sequences from BLV infected cattle. Phylogenetic analysis showed the affiliation of sequences to two already known genotypes G4 and G7 and also to a new genotype, classified as genotype G12. In addition, a multivariate method was employed for analysis of the association between proviral load and different variables such as the geographical location of the herd, cattle breeds, age of animals, and the presence of particular BLV genotypes. In summary, the results of this study provide the first evidence on molecular characterization of BLV circulating in cattle from Kazakhstan.https://www.mdpi.com/2076-0817/11/2/180bovine leukemia virus (BLV)BLV proviral loadphylogenetic analysisgenetic variabilityKazakhstan |
| spellingShingle | Akhmetzhan Sultanov Marzena Rola-Łuszczak Saltanat Mamanova Anna Ryło Zbigniew Osiński Meruyert A. Saduakassova Elvira Bashenova Jacek Kuźmak Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan Pathogens bovine leukemia virus (BLV) BLV proviral load phylogenetic analysis genetic variability Kazakhstan |
| title | Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan |
| title_full | Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan |
| title_fullStr | Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan |
| title_full_unstemmed | Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan |
| title_short | Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan |
| title_sort | molecular characterization of bovine leukemia virus with the evidence of a new genotype circulating in cattle from kazakhstan |
| topic | bovine leukemia virus (BLV) BLV proviral load phylogenetic analysis genetic variability Kazakhstan |
| url | https://www.mdpi.com/2076-0817/11/2/180 |
| work_keys_str_mv | AT akhmetzhansultanov molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan AT marzenarolałuszczak molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan AT saltanatmamanova molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan AT annaryło molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan AT zbigniewosinski molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan AT meruyertasaduakassova molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan AT elvirabashenova molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan AT jacekkuzmak molecularcharacterizationofbovineleukemiaviruswiththeevidenceofanewgenotypecirculatingincattlefromkazakhstan |