Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers
Cardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach to in vivo perfusion ima...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12591 |
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| author | Tomasz Jan Kolanowski Weronika Wargocka-Matuszewska Agnieszka Zimna Lukasz Cheda Joanna Zyprych-Walczak Anna Rugowska Monika Drabik Michał Fiedorowicz Seweryn Krajewski Łukasz Steczek Cezary Kozanecki Zbigniew Rogulski Natalia Rozwadowska Maciej Kurpisz |
| author_facet | Tomasz Jan Kolanowski Weronika Wargocka-Matuszewska Agnieszka Zimna Lukasz Cheda Joanna Zyprych-Walczak Anna Rugowska Monika Drabik Michał Fiedorowicz Seweryn Krajewski Łukasz Steczek Cezary Kozanecki Zbigniew Rogulski Natalia Rozwadowska Maciej Kurpisz |
| author_sort | Tomasz Jan Kolanowski |
| collection | DOAJ |
| description | Cardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach to in vivo perfusion imaging using the novel SYN1 tracer based on the fluorine-18 isotope. The NOD-SCID mice were injected intravenously with SYN1 or [<sup>18</sup>F] fluorodeoxyglucose ([<sup>18</sup>F]-FDG) radiotracers after induction of the MI. In all studies, the positron emission tomography–computed tomography (PET/CT) technique was used. To obtain hemodynamic data, mice were subjected to magnetic resonance imaging (MRI). Finally, the biodistribution of the SYN1 compound was performed using Wistar rat model. SYN1 showed normal accumulation in mouse and rat hearts, and MI hearts correctly indicated impaired cardiac segments when compared to [<sup>18</sup>F]-FDG uptake. In vivo PET/CT and MRI studies showed statistical convergence in terms of the size of the necrotic zone and cardiac function. This was further supported with RNAseq molecular analyses to correlate the candidate function genes’ expression, with <i>Serpinb1c</i>, <i>Tnc</i> and <i>Nupr1</i>, with <i>Trem2</i> and <i>Aldolase B</i> functional correlations showing statistical significance in both SYN1 and [<sup>18</sup>F]-FDG. Our manuscript presents a new fluorine-18-based perfusion radiotracer for PET/CT imaging that may have importance in clinical applications. Future research should focus on confirmation of the data elucidated here to prepare SYN1 for first-in-human trials. |
| first_indexed | 2024-03-10T05:24:34Z |
| format | Article |
| id | doaj.art-4ba5917e35394733b2e8d180cf88ea40 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:24:34Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4ba5917e35394733b2e8d180cf88ea402023-11-22T23:44:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221259110.3390/ijms222212591Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability TracersTomasz Jan Kolanowski0Weronika Wargocka-Matuszewska1Agnieszka Zimna2Lukasz Cheda3Joanna Zyprych-Walczak4Anna Rugowska5Monika Drabik6Michał Fiedorowicz7Seweryn Krajewski8Łukasz Steczek9Cezary Kozanecki10Zbigniew Rogulski11Natalia Rozwadowska12Maciej Kurpisz13Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandBiological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, 02-089 Warsaw, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandBiological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, 02-089 Warsaw, PolandDepartment of Mathematical and Statistical Methods, Poznan University of Life Sciences, 60-637 Poznan, PolandInstitute of Human Biology and Evolution, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, 02-106 Warsaw, PolandMossakowski Medical Research Institute, Polish Academy of Sciences, 02-106 Warsaw, PolandSynektik S.A., 00-728 Warsaw, PolandSynektik S.A., 00-728 Warsaw, PolandSynektik S.A., 00-728 Warsaw, PolandBiological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, 02-089 Warsaw, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandCardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach to in vivo perfusion imaging using the novel SYN1 tracer based on the fluorine-18 isotope. The NOD-SCID mice were injected intravenously with SYN1 or [<sup>18</sup>F] fluorodeoxyglucose ([<sup>18</sup>F]-FDG) radiotracers after induction of the MI. In all studies, the positron emission tomography–computed tomography (PET/CT) technique was used. To obtain hemodynamic data, mice were subjected to magnetic resonance imaging (MRI). Finally, the biodistribution of the SYN1 compound was performed using Wistar rat model. SYN1 showed normal accumulation in mouse and rat hearts, and MI hearts correctly indicated impaired cardiac segments when compared to [<sup>18</sup>F]-FDG uptake. In vivo PET/CT and MRI studies showed statistical convergence in terms of the size of the necrotic zone and cardiac function. This was further supported with RNAseq molecular analyses to correlate the candidate function genes’ expression, with <i>Serpinb1c</i>, <i>Tnc</i> and <i>Nupr1</i>, with <i>Trem2</i> and <i>Aldolase B</i> functional correlations showing statistical significance in both SYN1 and [<sup>18</sup>F]-FDG. Our manuscript presents a new fluorine-18-based perfusion radiotracer for PET/CT imaging that may have importance in clinical applications. Future research should focus on confirmation of the data elucidated here to prepare SYN1 for first-in-human trials.https://www.mdpi.com/1422-0067/22/22/12591fluorine radiotracersSYN1[<sup>18</sup>F]-FDGFDGpositron emission tomography–computed tomography (PET/CT)molecular profiling after myocardial infarction |
| spellingShingle | Tomasz Jan Kolanowski Weronika Wargocka-Matuszewska Agnieszka Zimna Lukasz Cheda Joanna Zyprych-Walczak Anna Rugowska Monika Drabik Michał Fiedorowicz Seweryn Krajewski Łukasz Steczek Cezary Kozanecki Zbigniew Rogulski Natalia Rozwadowska Maciej Kurpisz Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers International Journal of Molecular Sciences fluorine radiotracers SYN1 [<sup>18</sup>F]-FDG FDG positron emission tomography–computed tomography (PET/CT) molecular profiling after myocardial infarction |
| title | Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_full | Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_fullStr | Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_full_unstemmed | Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_short | Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([<sup>18</sup>F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers |
| title_sort | multiparametric evaluation of post mi small animal models using metabolic sup 18 sup f fdg and perfusion based syn1 heart viability tracers |
| topic | fluorine radiotracers SYN1 [<sup>18</sup>F]-FDG FDG positron emission tomography–computed tomography (PET/CT) molecular profiling after myocardial infarction |
| url | https://www.mdpi.com/1422-0067/22/22/12591 |
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