Rapid detection of penbutolol in pig urine using an immunochromatographic test strip
A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2018.1520203 |
| _version_ | 1818840078641790976 |
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| author | Wenliang Ge Steven Suryoprabowo Liguang Xu Qiankun Zheng Hua Kuang |
| author_facet | Wenliang Ge Steven Suryoprabowo Liguang Xu Qiankun Zheng Hua Kuang |
| author_sort | Wenliang Ge |
| collection | DOAJ |
| description | A rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test line and control line, respectively. Under optimised conditions, the semi-quantitative test strip detected PB at concentrations of 2.5 ng/mL in 0.01 M PBS (pH 7.4) and 0.5 ng/mL in pig urine. All results were obtained within 5 min. Our data indicate that this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of PB. |
| first_indexed | 2024-12-19T04:04:28Z |
| format | Article |
| id | doaj.art-4ba740fe79374755b67ce428ea357b1e |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-19T04:04:28Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-4ba740fe79374755b67ce428ea357b1e2022-12-21T20:36:33ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-012911126113610.1080/09540105.2018.15202031520203Rapid detection of penbutolol in pig urine using an immunochromatographic test stripWenliang Ge0Steven Suryoprabowo1Liguang Xu2Qiankun Zheng3Hua Kuang4Wuxi No.2 people’s hospitalState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityDelishi GroupState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityA rapid, simple, sensitive immunochromatographic strip test was developed to detect penbutolol (PB) in pig urine based on a sensitive and specific monoclonal antibody produced in our laboratory. The antigen (PB-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test line and control line, respectively. Under optimised conditions, the semi-quantitative test strip detected PB at concentrations of 2.5 ng/mL in 0.01 M PBS (pH 7.4) and 0.5 ng/mL in pig urine. All results were obtained within 5 min. Our data indicate that this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of PB.http://dx.doi.org/10.1080/09540105.2018.1520203β-blockerimmunochromatographic assaymonoclonal antibodypenbutololstrip test |
| spellingShingle | Wenliang Ge Steven Suryoprabowo Liguang Xu Qiankun Zheng Hua Kuang Rapid detection of penbutolol in pig urine using an immunochromatographic test strip Food and Agricultural Immunology β-blocker immunochromatographic assay monoclonal antibody penbutolol strip test |
| title | Rapid detection of penbutolol in pig urine using an immunochromatographic test strip |
| title_full | Rapid detection of penbutolol in pig urine using an immunochromatographic test strip |
| title_fullStr | Rapid detection of penbutolol in pig urine using an immunochromatographic test strip |
| title_full_unstemmed | Rapid detection of penbutolol in pig urine using an immunochromatographic test strip |
| title_short | Rapid detection of penbutolol in pig urine using an immunochromatographic test strip |
| title_sort | rapid detection of penbutolol in pig urine using an immunochromatographic test strip |
| topic | β-blocker immunochromatographic assay monoclonal antibody penbutolol strip test |
| url | http://dx.doi.org/10.1080/09540105.2018.1520203 |
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