Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt Stress
Ginseng (<i>Panax ginseng</i> C. A. Meyer), due to its relatively longer cultivation time, is often exposed to environmental stresses such as heat, salt, and drought. Particularly, salt-stress-derived oxidative damages greatly affect photosynthetic efficiency and consequently cause reduc...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/9/2048 |
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| author | Ju-Young Jung Cheol Woo Min So Wun Kim Ravi Gupta Woojong Jang Kyong-Hwan Bang Yu-Jin Kim Ick-Hyun Jo Sun Tae Kim |
| author_facet | Ju-Young Jung Cheol Woo Min So Wun Kim Ravi Gupta Woojong Jang Kyong-Hwan Bang Yu-Jin Kim Ick-Hyun Jo Sun Tae Kim |
| author_sort | Ju-Young Jung |
| collection | DOAJ |
| description | Ginseng (<i>Panax ginseng</i> C. A. Meyer), due to its relatively longer cultivation time, is often exposed to environmental stresses such as heat, salt, and drought. Particularly, salt-stress-derived oxidative damages greatly affect photosynthetic efficiency and consequently cause reduction of growth, development, and yield of ginseng. Thus, efforts have been made to understand the salt-stress-induced changes at proteome levels; however, the overall understanding of possible salt-responsive proteins in ginseng is still limited because of their low-abundance. A growing body of evidence suggests that plants secrete various low-abundant proteins localized in the intra- and extracellular spaces during stress conditions, and those proteins may have a key role for salt tolerance. Therefore, here, we report the ginseng fluids proteome to identify the potential salt-responsive proteins. This approach led to the identification of 261 secreted fluid proteins, and functional categorization revealed that identified proteins were majorly associated with photosynthesis, protein synthesis, cell binding, and various metabolisms. Further validation using qRT-PCR analysis showed similar expression profiles of heat-shock protein 70, glycosyl hydrolase 17, and fructose-bisphosphate aldolase class-I with proteome results. Overall, ginseng fluid proteomic analysis successfully identified the potential salt-responsive proteins, which might be helpful for understanding of salt-tolerance mechanisms in ginseng. |
| first_indexed | 2024-03-10T00:59:32Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T00:59:32Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-4bab995448cc49c28e4b90af06eea6e42023-11-23T14:36:29ZengMDPI AGAgronomy2073-43952022-08-01129204810.3390/agronomy12092048Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt StressJu-Young Jung0Cheol Woo Min1So Wun Kim2Ravi Gupta3Woojong Jang4Kyong-Hwan Bang5Yu-Jin Kim6Ick-Hyun Jo7Sun Tae Kim8Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaDepartment of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaCollege of General Education, Kookmin University, Seoul 02707, KoreaDepartment of Herbal Crop Research, Rural Development Administration, Eumseong 27709, KoreaDepartment of Herbal Crop Research, Rural Development Administration, Eumseong 27709, KoreaDepartment of Life Science and Envirmental Biochemistry, Pusan National University, Miryang 50463, KoreaDepartment of Herbal Crop Research, Rural Development Administration, Eumseong 27709, KoreaDepartment of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, KoreaGinseng (<i>Panax ginseng</i> C. A. Meyer), due to its relatively longer cultivation time, is often exposed to environmental stresses such as heat, salt, and drought. Particularly, salt-stress-derived oxidative damages greatly affect photosynthetic efficiency and consequently cause reduction of growth, development, and yield of ginseng. Thus, efforts have been made to understand the salt-stress-induced changes at proteome levels; however, the overall understanding of possible salt-responsive proteins in ginseng is still limited because of their low-abundance. A growing body of evidence suggests that plants secrete various low-abundant proteins localized in the intra- and extracellular spaces during stress conditions, and those proteins may have a key role for salt tolerance. Therefore, here, we report the ginseng fluids proteome to identify the potential salt-responsive proteins. This approach led to the identification of 261 secreted fluid proteins, and functional categorization revealed that identified proteins were majorly associated with photosynthesis, protein synthesis, cell binding, and various metabolisms. Further validation using qRT-PCR analysis showed similar expression profiles of heat-shock protein 70, glycosyl hydrolase 17, and fructose-bisphosphate aldolase class-I with proteome results. Overall, ginseng fluid proteomic analysis successfully identified the potential salt-responsive proteins, which might be helpful for understanding of salt-tolerance mechanisms in ginseng.https://www.mdpi.com/2073-4395/12/9/2048fluids proteomepanax ginsengsalt stressshotgun proteomics |
| spellingShingle | Ju-Young Jung Cheol Woo Min So Wun Kim Ravi Gupta Woojong Jang Kyong-Hwan Bang Yu-Jin Kim Ick-Hyun Jo Sun Tae Kim Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt Stress Agronomy fluids proteome panax ginseng salt stress shotgun proteomics |
| title | Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt Stress |
| title_full | Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt Stress |
| title_fullStr | Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt Stress |
| title_full_unstemmed | Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt Stress |
| title_short | Proteomic Analysis of Ginseng (<i>Panax ginseng</i> C. A. Meyer) Fluid Proteins under Salt Stress |
| title_sort | proteomic analysis of ginseng i panax ginseng i c a meyer fluid proteins under salt stress |
| topic | fluids proteome panax ginseng salt stress shotgun proteomics |
| url | https://www.mdpi.com/2073-4395/12/9/2048 |
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