Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line
A typical DYT1 dystonia is caused by a heterozygous GAG deletion (c.907–909) in the TOR1A gene (ΔE, p.Glu303del) and the pathogenesis is not clear. In this study, human induced pluripotent stem cell (hiPSC) lines carrying the heterozygous or homozygous GAG deletion in TOR1A gene were generated by ge...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121003834 |
| _version_ | 1818838007227088896 |
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| author | Masuma Akter Haochen Cui Yi-Hsien Chen Baojin Ding |
| author_facet | Masuma Akter Haochen Cui Yi-Hsien Chen Baojin Ding |
| author_sort | Masuma Akter |
| collection | DOAJ |
| description | A typical DYT1 dystonia is caused by a heterozygous GAG deletion (c.907–909) in the TOR1A gene (ΔE, p.Glu303del) and the pathogenesis is not clear. In this study, human induced pluripotent stem cell (hiPSC) lines carrying the heterozygous or homozygous GAG deletion in TOR1A gene were generated by genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A). These hiPSC lines showed the normal stem cell morphology and karyotype, expressed the same pluripotency markers as their parental line, and had the capacity to differentiate into three germ layers, providing a valuable resource in determining the pathogenesis of human DYT1 dystonia. |
| first_indexed | 2024-12-19T03:31:33Z |
| format | Article |
| id | doaj.art-4bacd8f5dfcc402ba1dbcbcb608ca8df |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-19T03:31:33Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-4bacd8f5dfcc402ba1dbcbcb608ca8df2022-12-21T20:37:29ZengElsevierStem Cell Research1873-50612021-10-0156102536Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC lineMasuma Akter0Haochen Cui1Yi-Hsien Chen2Baojin Ding3Department of Biology, University of Louisiana at Lafayette, 410 East Saint Mary Boulevard, Lafayette LA 70503, USADepartment of Biology, University of Louisiana at Lafayette, 410 East Saint Mary Boulevard, Lafayette LA 70503, USAGenome Engineering and iPSC Center (GEiC), Washington University at St Louis, MO 63110, USADepartment of Biology, University of Louisiana at Lafayette, 410 East Saint Mary Boulevard, Lafayette LA 70503, USA; Corresponding author.A typical DYT1 dystonia is caused by a heterozygous GAG deletion (c.907–909) in the TOR1A gene (ΔE, p.Glu303del) and the pathogenesis is not clear. In this study, human induced pluripotent stem cell (hiPSC) lines carrying the heterozygous or homozygous GAG deletion in TOR1A gene were generated by genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A). These hiPSC lines showed the normal stem cell morphology and karyotype, expressed the same pluripotency markers as their parental line, and had the capacity to differentiate into three germ layers, providing a valuable resource in determining the pathogenesis of human DYT1 dystonia.http://www.sciencedirect.com/science/article/pii/S1873506121003834 |
| spellingShingle | Masuma Akter Haochen Cui Yi-Hsien Chen Baojin Ding Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line Stem Cell Research |
| title | Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line |
| title_full | Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line |
| title_fullStr | Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line |
| title_full_unstemmed | Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line |
| title_short | Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line |
| title_sort | generation of two induced pluripotent stem cell lines with heterozygous and homozygous gag deletion in tor1a gene from a healthy hipsc line |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121003834 |
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