Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVB
Peng-Cheng Zhang,1,2 Yi Hong,3 Shi-Qin Zong,1,2 Long Chen,1,2 Chong Zhang,4 Dai-Zhi Tian,4 Dan Ke,5 Li-Ming Tian1,2,4 1Department of Dermatology, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2023-11-01
|
| Series: | Clinical, Cosmetic and Investigational Dermatology |
| Subjects: | |
| Online Access: | https://www.dovepress.com/variation-of-ferroptosis-related-markers-in-hacat-cell-photoaging-mode-peer-reviewed-fulltext-article-CCID |
| _version_ | 1797640466199478272 |
|---|---|
| author | Zhang PC Hong Y Zong SQ Chen L Zhang C Tian DZ Ke D Tian LM |
| author_facet | Zhang PC Hong Y Zong SQ Chen L Zhang C Tian DZ Ke D Tian LM |
| author_sort | Zhang PC |
| collection | DOAJ |
| description | Peng-Cheng Zhang,1,2 Yi Hong,3 Shi-Qin Zong,1,2 Long Chen,1,2 Chong Zhang,4 Dai-Zhi Tian,4 Dan Ke,5 Li-Ming Tian1,2,4 1Department of Dermatology, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China; 2Hubei Provincial Key Laboratory of Skin Infection and Immunity, Wuhan No.1 Hospital, Wuhan, 430022, People’s Republic of China; 3College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People’s Republic of China; 4Institute of Geriatrics, Hubei University of Chinese Medicine, Wuhan, 430065, People’s Republic of China; 5Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400000, People’s Republic of ChinaCorrespondence: Dan Ke, Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, No. 40 of Daomenkou, Yuzhong District, Chongqing, 400000, People’s Republic of China, Tel +86 23 6390 1120, Fax +86 23 6373 1325, Email 2306896229@qq.com Li-Ming Tian, Department of Dermatology, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, No. 215 of Zhongshan Avenue, Qiaokou District, Wuhan, 430022, People’s Republic of China, Tel +86 27 8533 2606, Fax +86 27 8583 2563, Email jolly0731@126.comObjective: To investigate the variation of ferroptosis-related markers in HaCaT cell photoaging models induced by ultraviolet-B (UVB).Methods: UVB-treated HaCaT cells served as the model (UVB group) for cellular photoaging, whereas untreated HaCaT cells served as the control group. HaCaT cells were exposed to UVB and the ferroptosis inhibitor Ferrostatin-1 (Fer-1) as part of the UVB+Fer-1 group, and co-cultured with the ferroptosis inducer Erastin as part of the UVB+Erastin group. Reactive oxygen species (ROS) detection kit and senescence-related β galactosidase (SA-β-gal) staining were used to evaluate the senescence of HaCaT cells. Lipid reactive oxygen species were detected by C11 BODIPY581/591 probe and mitochondrial morphology was observed by transmission electron microscopy. The mRNA expressions of glutathione peroxidase 4 (GPX4) and ferroptosis-suppressor-protein 1 (FSP1) were detected by real-time reverse transcription-PCR (RT-RCP), and the level of GPX4 protein was measured by immunofluorescence assay.Results: The UVB group had considerably greater levels of ROS, SA-β-gal, and lipid reactive oxygen species than the control group. The UVB group’s mitochondrial volume was reduced, the membrane density increased, and the mitochondrial crest decreased or even disappeared. GPX4 and FSP1 expression levels were similarly found to be lower in the UVB group. Furthermore, the positive rate of SA-β-gal and lipid reactive oxygen species in the UVB+Fer-1 group was much lower than in the UVB group, but it was reverse in the UVB+Erastin group. This study showed that induced ferroptosis can aggravate aging, and vice versa.Conclusion: According to the findings, ferroptosis may be linked to UVB-induced skin photoaging, which could be attenuated by inhibition of ferroptosis.Keywords: skin aging, ferroptosis, oxidative stress |
| first_indexed | 2024-03-11T13:32:17Z |
| format | Article |
| id | doaj.art-4bacdb78138d4b0cbee977ffab9a224a |
| institution | Directory Open Access Journal |
| issn | 1178-7015 |
| language | English |
| last_indexed | 2024-03-11T13:32:17Z |
| publishDate | 2023-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj.art-4bacdb78138d4b0cbee977ffab9a224a2023-11-02T17:13:56ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152023-11-01Volume 163147315587776Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVBZhang PCHong YZong SQChen LZhang CTian DZKe DTian LMPeng-Cheng Zhang,1,2 Yi Hong,3 Shi-Qin Zong,1,2 Long Chen,1,2 Chong Zhang,4 Dai-Zhi Tian,4 Dan Ke,5 Li-Ming Tian1,2,4 1Department of Dermatology, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China; 2Hubei Provincial Key Laboratory of Skin Infection and Immunity, Wuhan No.1 Hospital, Wuhan, 430022, People’s Republic of China; 3College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People’s Republic of China; 4Institute of Geriatrics, Hubei University of Chinese Medicine, Wuhan, 430065, People’s Republic of China; 5Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400000, People’s Republic of ChinaCorrespondence: Dan Ke, Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, No. 40 of Daomenkou, Yuzhong District, Chongqing, 400000, People’s Republic of China, Tel +86 23 6390 1120, Fax +86 23 6373 1325, Email 2306896229@qq.com Li-Ming Tian, Department of Dermatology, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, No. 215 of Zhongshan Avenue, Qiaokou District, Wuhan, 430022, People’s Republic of China, Tel +86 27 8533 2606, Fax +86 27 8583 2563, Email jolly0731@126.comObjective: To investigate the variation of ferroptosis-related markers in HaCaT cell photoaging models induced by ultraviolet-B (UVB).Methods: UVB-treated HaCaT cells served as the model (UVB group) for cellular photoaging, whereas untreated HaCaT cells served as the control group. HaCaT cells were exposed to UVB and the ferroptosis inhibitor Ferrostatin-1 (Fer-1) as part of the UVB+Fer-1 group, and co-cultured with the ferroptosis inducer Erastin as part of the UVB+Erastin group. Reactive oxygen species (ROS) detection kit and senescence-related β galactosidase (SA-β-gal) staining were used to evaluate the senescence of HaCaT cells. Lipid reactive oxygen species were detected by C11 BODIPY581/591 probe and mitochondrial morphology was observed by transmission electron microscopy. The mRNA expressions of glutathione peroxidase 4 (GPX4) and ferroptosis-suppressor-protein 1 (FSP1) were detected by real-time reverse transcription-PCR (RT-RCP), and the level of GPX4 protein was measured by immunofluorescence assay.Results: The UVB group had considerably greater levels of ROS, SA-β-gal, and lipid reactive oxygen species than the control group. The UVB group’s mitochondrial volume was reduced, the membrane density increased, and the mitochondrial crest decreased or even disappeared. GPX4 and FSP1 expression levels were similarly found to be lower in the UVB group. Furthermore, the positive rate of SA-β-gal and lipid reactive oxygen species in the UVB+Fer-1 group was much lower than in the UVB group, but it was reverse in the UVB+Erastin group. This study showed that induced ferroptosis can aggravate aging, and vice versa.Conclusion: According to the findings, ferroptosis may be linked to UVB-induced skin photoaging, which could be attenuated by inhibition of ferroptosis.Keywords: skin aging, ferroptosis, oxidative stresshttps://www.dovepress.com/variation-of-ferroptosis-related-markers-in-hacat-cell-photoaging-mode-peer-reviewed-fulltext-article-CCIDskin agingferroptosisoxidative stress |
| spellingShingle | Zhang PC Hong Y Zong SQ Chen L Zhang C Tian DZ Ke D Tian LM Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVB Clinical, Cosmetic and Investigational Dermatology skin aging ferroptosis oxidative stress |
| title | Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVB |
| title_full | Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVB |
| title_fullStr | Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVB |
| title_full_unstemmed | Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVB |
| title_short | Variation of Ferroptosis-Related Markers in HaCaT Cell Photoaging Models Induced by UVB |
| title_sort | variation of ferroptosis related markers in hacat cell photoaging models induced by uvb |
| topic | skin aging ferroptosis oxidative stress |
| url | https://www.dovepress.com/variation-of-ferroptosis-related-markers-in-hacat-cell-photoaging-mode-peer-reviewed-fulltext-article-CCID |
| work_keys_str_mv | AT zhangpc variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb AT hongy variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb AT zongsq variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb AT chenl variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb AT zhangc variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb AT tiandz variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb AT ked variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb AT tianlm variationofferroptosisrelatedmarkersinhacatcellphotoagingmodelsinducedbyuvb |